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A multi-channel prismatic localized surface plasmon resonance (LSPR) biosensor was developed for quantitative and real-time detection of multiple COVID-19 characteristic miRNAs. The well-dispersed and dense gold nanoparticles (AuNPs) arrays for LSPR biosensing were fabricated through a nano-thickness diblock copolymer template (BCPT). Both theoretical and experimental analyses were conducted to investigate the effects of particle size, interparticle spacing, and surface coverage on LSPR sensing spectrum and intensity sensitivity of varied AuNPs sizes. A competitive assay strategy was proposed and used for non-amplification miRNA detection with a low limit detection of 3.41 nM, while a four-channel prismatic LSPR system enables parallel detection of multiple miRNAs. Furthermore, this sensing strategy can effectively and specifically identify target miRNA, distinguish mismatched miRNA and interfering miRNA, and exhibit low non-specific adsorption. This BCPT-based LSPR biosensor demonstrates the practicality and potential of a multi-channel, adaptable, and integrated prismatic sensor in medical testing and diagnostic applications., Competing Interests: Declaration of competing interest The authors declare the following no financial interests/personal relationships which may be considered as potential competing interests: Mengdi Lu reports financial support was provided by National Natural Science Foundation of China. Mengdi Lu reports financial support was provided by Liaoning Cancer Hospital Oncology + Funds. Mengdi Lu reports financial support was provided by Natural Science Foundation of Liaoning Province. Mengdi Lu reports financial support was provided by Fundamental Research Funds for the Central Universities. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Localized surface plasmon resonance (LSPR) biosensors, which enable nanoscale confinement and manipulation of light, offer the enhanced sensitivity and electromagnetic energy localization. The integration of LSPR with the fiber-optic technology has led to the development of compact and versatile sensors for miniaturization and remote sensing. This comprehensive review explores various sensor configurations, fiber types, and geometric shapes, highlighting their benefits in terms of sensitivity, integration, and performance improvement. Fabrication techniques such as focused non-chemical bonding strategies and self-assembly of nanoparticles are discussed, providing control over nanostructure morphology and enhancing sensor performance. Bio-applications of fiber-optic LSPR (FOLSPR) sensors are detailed, specifically in biomolecular interactions and analysis of proteins, pathogens and cells, nucleic acids (DNA and RNA), and other small molecules (organic compounds and heavy metal ions). Surface modification and detection schemes are emphasized for their potential for label-free and real-time biosensing. The challenges and prospects of FOLSPR sensors are addressed, including the developments in sensitivity, fabrication techniques, and measurement reliability. Integration with emerging technologies such as nanomaterials is highlighted as a promising direction for future research. Overall, this review provides insights into the advancements and potential applications of FOLSPR sensors, paving the way for sensitive and versatile optical biosensing platforms in various fields. [ABSTRACT FROM AUTHOR]