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Both MUC1 and MUC4 are high molecular weight glycoproteins and are independent indicators of worse prognosis in many human epithelial cancers including oral squamous cell carcinoma (OSCC). However, there has been no investigation of the clinical importance of the co-expression of MUC1 and MUC4 in OSCC. The aim of this study was to evaluate the co-expression profile of MUC1/MUC4 and analyze the prognostic significance in OSCC. We examined the expression profile of MUC1 and MUC4 in OSCC tissues from 206 patients using immunohistochemistry. The co-expression profile of MUC1/MUC4 and its prognostic significance in OSCC was statistically analyzed. MUC1 and MUC4 overexpression were strongly correlated with each other (p

Myofibroblastoma is a rare benign stromal neoplasm with myofibroblastic differentiation most often seen in the breast. In the oral region, only one case of myofibroblastoma has been reported so far; therefore, the diagnostic criteria of this tumor are still controversial in the field of oral pathology. Herein, the authors present a case of myofibroblastoma of the tongue and demonstrate the usefulness of immunohistochemistry in diagnosing this tumor. Histologically, the tumor was composed of interlacing bundles of spindle-shaped or oval cells with hyalinized stroma and amianthoid fibers. Immunohistochemically, the tumor cells showed characteristic features of mammary type myofibroblastoma: diffuse immunoreactivity for desmin, vimentin, and Bcl-2; focal immunoreactivity for α-smooth muscle actin, CD34, and cyclin D1; and no immunoreactivity for h-caldesmon. The pathological diagnosis of myofibroblastoma was made. The authors propose myofibroblastoma as a distinctive entity in the oral region, which should not be confused with other spindle-cell tumors or hamartomatous proliferation with myofibroblastic differentiation, and recommend the inclusion of extramammary myofibroblastoma in the differential diagnosis of soft tissue tumors of the oral cavity.

Purpose To prove that chromatin immunoprecipitation assay can be performed with oral rinse samples and to develop a protocol for comprehensive analysis of functional interactions among DNA methylation, histone modification, and gene expression using such samples. Materials and Methods Eleven cancer cell lines and oral rinse samples from 10 patients with oral squamous cell carcinoma and 3 healthy subjects were examined. The expression of CDKN2A , a tumor suppressor gene, was determined by reverse transcription/polymerase chain reaction and immunohistochemistry. Promoter DNA methylation was assessed by methylation-specific polymerase chain reaction. Chromatin modifications were analyzed by a chromatin immunoprecipitation assay using antibodies for dimethylation and acetylation of lysine 9 of histone H3. Results Epigenetic control of CDK2NA was observed in vitro in 11 cancer cell lines. Using the present protocol, comprehensive epigenetic analysis could be successfully performed with oral rinse samples. All patients were comfortable using the prescribed amount (16 mL) of normal saline to rinse their mouths. Nine patients (90%) and 1 healthy subject (33%) showed dimethylation of lysine 9 of histone H3. Moreover, 8 patients (80%) showed hypoacetylation of lysine 9 of histone H3, which was not observed in healthy subjects. Conclusions The present study showed for the first time that chromatin modifications can be analyzed using oral rinse samples by chromatin immunoprecipitation analysis. To evaluate the contribution of histone modifications for carcinogenesis of oral squamous cell carcinoma, studies including a larger number of subjects should be conducted in the future.

Improved treatment of elderly inpatients requires evaluation of their systemic status. We performed open reduction of a mandibular fracture under general anesthesia in a patient who had various underlying diseases, such as hypertension, emphysema, cerebral infarction, diabetes mellitus, and dementia.The patient had presented with dementia before hospitalization. To ensure safety, we paid attention to falling, which had caused the mandibular fracture. However, coronary arterial spasm unexpectedly occurred during operation under general anesthesia. After the planned operation, we prevented the onset of spastic angina, and the patient was discharged without any complications. It is essential to carefully confirm systemic status, including emerging symptoms and disease, to ensure safe perioperative management in elderly patients with many underlying diseases such as hypertension, cerebral infarction, and dementia.

Benign cementoblastoma is a very rare odontogenic tumor. A case of benign cementoblas-tomawith a severe percussion pain in the right molar region of the maxilla is reported. The patient wasa 16-year old male. He underwent resection of the tumor and has an excellent prognosis.

Beckwith-Wiedemann syndrome (BWS) is a congenital disease characterized by exomphalos, macroglossia and gigantism. This paper presents a case of BWS undergoingtongue reduction at the age of six years. The patient was a three-month-old baby referredto our clinic for macroglossia from the Department of Pediatrics, Kagoshima University Hos-pital. His tongue showed hemihypertrophic macroglossia on the right side with only slightdifficulty in secretion. In our case, macroglossia induced prognathism and openbite as thepatient became older. We therefore feel that tongue reduction should be performed at theage of about one year and six months for congenital macroglossia.

ion DNA from Candida species and PCR Mouth washing (10ml PBS) 10μl Harris Uni – core punch Φ1.25mm Whatman® SNET Tris・Hcl ph8.0 EDTA Nacl SDS Proteinase K Solution buffer + 55°C 3hours heating Primer Whatman® Card