1. Clinically compliant spatial and temporal imaging of chimeric antigen receptor T-cells.
- Author
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Emami-Shahri, Nia, Foster, Julie, Kashani, Roxana, Gazinska, Patrycja, Cook, Celia, Sosabowski, Jane, Maher, John, and Papa, Sophie
- Subjects
CHIMERIC antigen receptors ,T cells ,T cell receptors ,SODIUM iodide - Abstract
The unprecedented efficacy of chimeric antigen receptor (CAR) T-cell immunotherapy of CD19
+ B-cell malignancy has established a new therapeutic pillar of hematology-oncology. Nonetheless, formidable challenges remain for the attainment of comparable success in patients with solid tumors. To accelerate progress and rapidly characterize emerging toxicities, systems that permit the repeated and non-invasive assessment of CAR T-cell biodistribution would be invaluable. An ideal solution would entail the use of a non-immunogenic reporter that mediates specific uptake of an inexpensive, non-toxic and clinically established imaging tracer by CAR T cells. Here we show the utility of the human sodium iodide symporter (hNIS) for the temporal and spatial monitoring of CAR T-cell behavior in a cancerbearing host. This system provides a clinically compliant toolkit for high-resolution serial imaging of CAR T cells in vivo, addressing a fundamental unmet need for future clinical development in the field. [ABSTRACT FROM AUTHOR]- Published
- 2018
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