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Your search keyword '"D. Gennari"' showing total 6 results
Start Over Author "D. Gennari" Topic t-lymphocyte subsets
6 results on '"D. Gennari"'

1. Phenotypic profile and functional characteristics of human gamma and delta T cells during acute toxoplasmosis.

Author

De Paoli P, Basaglia G, Gennari D, Crovatto M, Modolo ML, and Santini G

Subjects
Acute Disease, Adult, Animals, Antigens, Protozoan, Female, Humans, In Vitro Techniques, Lymphocyte Activation, Male, Phenotype, Toxoplasma immunology, Receptors, Antigen, T-Cell, gamma-delta, T-Lymphocyte Subsets immunology, Toxoplasmosis immunology
Abstract

Gamma and delta (gamma delta) T-cell receptor lymphocytes are increased during acute toxoplasmosis. These cells are BB3+ CD45RO+ CD8-. Purified gamma delta T cells failed to proliferate in response to Toxoplasma gondii antigen (stimulation index, 1.4 +/- 0.6) but were responsive to phytohemagglutinin stimulation (stimulation index, 20.8 +/- 1.9). Natural-killer-like cytotoxicity was strongly acquired only after in vitro culture of purified gamma delta T cells with recombinant interleukin 2 (40% +/- 7% specific lysis). Our data show that gamma delta T-cell receptor T cells with a peculiar phenotype are increased during human acute T. gondii infection.

Published
1992
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2. Gamma delta T cells in infectious diseases.

Author

de Paoli P, Basaglia G, Gennari D, and Santini G

Subjects
Animals, Antigens, Bacterial immunology, Antigens, Differentiation, T-Lymphocyte immunology, CD3 Complex, Gene Rearrangement, delta-Chain T-Cell Antigen Receptor, Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor, Humans, Mice, Mycobacterium immunology, Organ Specificity, Receptors, Antigen, T-Cell immunology, Sepsis immunology, Species Specificity, Infections immunology, Receptors, Antigen, T-Cell, gamma-delta, T-Lymphocyte Subsets immunology
Published
1991

3. CD8 lymphocytes during Epstein Barr virus (EBV) infection: A CD29 positive population is expanded in acute infectious mononucleosis.

Author

de Paoli P, Gennari D, Reitano M, Martelli P, Sacilotto C, Comoretto R, and Santini G

Subjects
Acute Disease, Antibodies, Monoclonal immunology, Convalescence, Herpesvirus 4, Human, Humans, Integrin beta1, Antigens, CD analysis, CD8 Antigens analysis, Infectious Mononucleosis immunology, T-Lymphocyte Subsets immunology, T-Lymphocytes, Regulatory immunology
Abstract

The expression of phenotypic markers on CD4 and CD8 lymphocytes during the acute and convalescent phases of Epstein Barr virus (EBV) induced infectious mononucleosis was examined by two colour flow cytometry. Activated CD8 cells constitute the major population increased during acute infectious mononucleosis; in this phase we observed a preferential expansion of the CD8 CD29+ compared to the CD8 CD45RA+ cells. Serum soluble CD8 levels were also raised during the acute phase and a correlation with CD8 CD38+ and CD8 CD29+ cell numbers was found. The convalescent phase of infectious mononucleosis was characterized by a progressive return of CD8 subset and of soluble CD8 to baseline normal values. These results demonstrate that acute EBV infection induces the expansion of a CD8 subset with peculiar surface antigenic profile.

Published
1991

4. Gamma delta T cells in infectious diseases

Author

P, de Paoli, G, Basaglia, D, Gennari, and G, Santini

Subjects
Antigens, Differentiation, T-Lymphocyte, Antigens, Bacterial, CD3 Complex, Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor, Gene Rearrangement, delta-Chain T-Cell Antigen Receptor, Receptors, Antigen, T-Cell, Receptors, Antigen, T-Cell, gamma-delta, Infections, Mycobacterium, Mice, Species Specificity, Organ Specificity, T-Lymphocyte Subsets, Sepsis, Animals, Humans
Published
1991

5. CD8 lymphocytes during Epstein Barr virus (EBV) infection: A CD29 positive population is expanded in acute infectious mononucleosis

Author

P, de Paoli, D, Gennari, M, Reitano, P, Martelli, C, Sacilotto, R, Comoretto, and G, Santini

Subjects
Herpesvirus 4, Human, Antigens, CD, T-Lymphocyte Subsets, CD8 Antigens, Integrin beta1, Acute Disease, Antibodies, Monoclonal, Humans, Convalescence, Infectious Mononucleosis, T-Lymphocytes, Regulatory
Abstract

The expression of phenotypic markers on CD4 and CD8 lymphocytes during the acute and convalescent phases of Epstein Barr virus (EBV) induced infectious mononucleosis was examined by two colour flow cytometry. Activated CD8 cells constitute the major population increased during acute infectious mononucleosis; in this phase we observed a preferential expansion of the CD8 CD29+ compared to the CD8 CD45RA+ cells. Serum soluble CD8 levels were also raised during the acute phase and a correlation with CD8 CD38+ and CD8 CD29+ cell numbers was found. The convalescent phase of infectious mononucleosis was characterized by a progressive return of CD8 subset and of soluble CD8 to baseline normal values. These results demonstrate that acute EBV infection induces the expansion of a CD8 subset with peculiar surface antigenic profile.

Published
1991

6. A subset of gamma delta lymphocytes is increased during HIV-1 infection

Author

P, De Paoli, D, Gennari, P, Martelli, G, Basaglia, M, Crovatto, S, Battistin, and G, Santini

Subjects
Antigens, Differentiation, T-Lymphocyte, T-Lymphocyte Subsets, CD8 Antigens, T-Lymphocytes, HIV-1, Receptors, Antigen, T-Cell, Humans, HIV Infections, Receptors, Antigen, T-Cell, gamma-delta, Research Article
Abstract

The gamma delta T cell receptor (TcR) lymphocytes constitute 3-10% of human peripheral blood lymphocytes. Only a very small fraction of these cells is recognized by the delta TCS1 monoclonal antibody, directed against the V delta 1 chain of the receptor. We describe the immunological, virological and clinical data of a small group of seropositive subjects having high levels of gamma delta TcR T cells in the peripheral blood. Our flow cytometric studies show that most of these cells belong to the delta TCS1+ (V delta 1+), CD8 +/- (dim staining) subset. Patients with high gamma delta TcR T cell numbers were not characterized by the presence of an acute (IgM positive) or reactivated (as defined by high IgG titres against early antigen or IgA titres against viral capsidic antigen) Epstein-Barr virus infection. Cytomegalovirus infection was excluded by serological assays, and other herpes viral infections were not found after clinical examination. HIV p24 antigenaemia was present in two out of 11 subjects. AIDS patients had very high percentages of gamma delta TcR T cells. Altogether these data show that the selective expansion of delta TCS1+ cells in HIV1 seropositive subjects is not related to some exogenous antigen stimulation, but may be related to peculiar pathologic processes involving the immune system.

Published
1991

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