1. Protein-tannic acid multilayer films: A multifunctional material for microencapsulation of food-derived bioactives.
- Author
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Lau HH, Murney R, Yakovlev NL, Novoselova MV, Lim SH, Roy N, Singh H, Sukhorukov GB, Haigh B, and Kiryukhin MV
- Subjects
- Adsorption, Animals, Caco-2 Cells, Capsules, Cattle, Gastric Juice chemistry, Humans, Immunoglobulin G chemistry, Lactoglobulins chemistry, Serum Albumin, Bovine chemistry, Biocompatible Materials, Drug Delivery Systems, Immunoglobulin G administration & dosage, Lactoglobulins administration & dosage, Serum Albumin, Bovine administration & dosage, Tannins chemistry
- Abstract
The benefits of various functional foods are often negated by stomach digestion and poor targeting to the lower gastrointestinal tract. Layer-by-Layer assembled protein-tannic acid (TA) films are suggested as a prospective material for microencapsulation of food-derived bioactive compounds. Bovine serum albumin (BSA)-TA and pepsin-TA films demonstrate linear growth of 2.8±0.1 and 4.2±0.1nm per bi-layer, correspondingly, as shown by ellipsometry. Both multilayer films are stable in simulated gastric fluid but degrade in simulated intestinal fluid. Their corresponding degradation constants are 0.026±0.006 and 0.347±0.005nm
-1 min-1 . Milk proteins possessing enhanced adhesion to human intestinal surface, Immunoglobulin G (IgG) and β-Lactoglobulin (BLG), are explored to tailor targeting function to BSA-TA multilayer film. BLG does not adsorb onto the multilayer while IgG is successfully incorporated. Microcapsules prepared from the multilayer demonstrate 2.7 and 6.3 times higher adhesion to Caco-2 cells when IgG is introduced as an intermediate and the terminal layer, correspondingly. This developed material has a great potential for oral delivery of numerous active food-derived ingredients., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2017
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