Search

The genus Poeonoma (Tams & Bowden, 1953) is revised to include two species P. serrata (Hampson, 1910) and P. ugandensis Le Run. sp., from the Congolian bioregion. Several new synonyms are introduced for Poeonoma serrata: Conicofrontia sjoestedti (Aurivillius, 1925) n. syn. for Poeonoma serrata (Hampson, 1910), Poeonoma similis (Tams & Bowden, 1953) n. syn. for Poeonoma serrata (Hampson, 1910), and Poeonoma nigribasis (Laporte, 1974) n. syn. for Poeonoma serrata (Hampson, 1910). A new genus, Nyaluteme Le Run. gen., is proposed for two species formerly included in Poeonoma, N. acantha (Tams & Bowden, 1953) n. comb. and N. inermis (Laporte, 1973) n. comb., and N. nigra Le Run. sp., described from the Congolian bioregion. Host-plants for three of the species are recorded:Pennisetum purpureum Schumach. for P. serrata and P. ugandensis, and Miscanthus violaceus (K. Schum) Pilg. for N. nigra. A key to species, descriptions, illustrations of adults and genitalia, and distribution maps are included. Results of previously published phylogenetic analyses also allow the status of the new genus to be confirmed and to infer the phylogenetic placement of the two genera.; Le genre Poeonoma (Tams & Bowden, 1953) est révisé ; deux espèces, P. serrata (Hampson, 1910) et P. ugandensis Le Ru n. sp., de la biorégion Congolaise, y sont incluses et plusieurs nouvelles synonymies sont proposées pour Poeonoma serrata : Conicofrontia sjoestedti (Aurivillius, 1925) n. syn. de Poeonoma serrata (Hampson, 1910), Poeonoma similis (Tams & Bowden, 1953) n. syn. de Poeonoma serrata (Hampson, 1910), et Poeonoma nigribasis (Laporte, 1974) n. syn. de Poeonoma serrata (Hampson, 1910). Un nouveau genre, Nyaluteme Le Ru n. gen., de la biorégion Congolaise, est proposé pour deux espèces précédemment décrites dans le genre Poeonoma, N. acantha (Tams & Bowden, 1953) n. comb., et N. inermis (Laporte, 1973) n. comb., et une espèce nouvellement décrite N. nigra Le Ru n. sp. Les plantes-hôtes de trois espèces sont répertoriées : Pennisetum purpureum Schumach. pour P. serrata et P. ugandensis, et Miscanthus violaceus (K. Schum) Pilg. pour N. nigra. Une clé de détermination des espèces est présentée ainsi que l’illustration des adultes et des génitalia et, des cartes de distribution. Les résultats d’analyses de reconstruction phylogénétique déjà publiées permettent également de confirmer le statut du nouveau genre et d’inférer la position phylogénétique des deux genres. http://www.zoobank.org/urn:lsid:zoobank.org:pub:6ED4AC9E-043C-4A04-A92D-807066943002

International audience; The genus Acrapex Hampson, 1891 (Lepidoptera, Noctuidae, Noctuinae, Apameini, Sesamiina) constitutes a speciose group of noctuid stemborers mostly distributed in the Afrotropics. In this study nine new Acrapex species are described: Acrapex abyssinica n. sp., Acrapex dabaga n. sp., Acrapex jansei n. sp., Acrapex kifanya n. sp., Acrapex lusinga n. sp., Acrapex ngwenya n. sp., Acrapex njombea n. sp., Acrapex vetiveria n. sp. and Acrapex zima n. sp. All species are assigned to the A. albivena group with the exception of A. lusinga which is assigned to the A. stygiata group. We also provide supplemental descriptions for two previously described species of the A. albivena group, A. punctosa Berio, 1973 and A. sysciodes Berio, 1973, and for one species belonging to the A. stygiata group: A. brunneella Le Ru, 2014. Host plants of three species are recorded; A. brunneella and Acrapex jansei were reared on Cymbopogon pospischilii (K.Schum.) C.E.Hubb and A. vetiveria on Chrysopogon zizanioides (L.). We also conducted molecular phylogenetic analyses (using both Bayesian inference and maximum likelihood) on a multi-marker (four mitochondrial and two nuclear genes) molecular dataset encompassing 138 specimens (including 98 specimens from the A. albivena group and 23 specimens from the A. stygiata group) from 48 stemborer species. The results of the corresponding analyses support the monophyly of the two groups of interest and the species status of all newly described taxa, except for A. lusinga that was not sequenced. The phylogenetic analyses also unravel several evolutionary lineages whose precise status is pending because their DNA was extracted from larval stages.; Actualisation des connaissances de la phylogénie et de la systématique des groupes d’espèces Acrapex albivena Hampson, 1910 et A. stygiata (Hampson, 1910) (Lepidoptera, Noctuidae, Noctuinae, Apameini, Sesamiina), avec la description de neuf nouvelles espèces de la région Afrotropicale. Le genre Acrapex Hampson, 1891 (Lepidoptera, Noctuidae, Noctuinae, Apameini, Sesamiina) est un groupe diversifié de noctuelles foreuses, majoritairement distribué dans la région Afrotropicale. Dans cette étude neuf espèces nouvelles sont décrites : Acrapex abyssinica n. sp., Acrapex dabaga n. sp., Acrapex jansei n. sp., Acrapex kifanya n. sp., A. lusinga n. sp., Acrapex ngwenya n. sp., Acrapex njombea n. sp., Acrapex vetiveria n. sp. et Acrapex zima n. sp. Toutes ces espèces sont assignées au groupe d’espèce A. albivena à l’exception d’A. lusinga qui est assignée au groupe d’espèce A. stygiata. Nous fournissons également des descriptions supplémentaires pour deux espèces, A. punctosa Berio, 1973 et A. sysciodes, Berio 1973, précedemment décrites dans le groupe A. albivena ainsi que pour A. brunneella Le Ru, 2014, une espèce qui appartient au groupe A. stygiata. Les plantes-hôtes de trois espèces sont répertoriées ; Acrapex brunneella et A. jansei ont été élevées sur Cymbopogon pospischilii (K. Schum.) C. E. Hubb et A. vetiveria sur Chrysopogon zizanioides (L.). Nous avons également réalisé des analyses de reconstruction phylogénétique (utilisant à la fois l’inférence Bayésienne et le maximum de vraisemblance) sur un jeu de données moléculaires multi-marqueurs (quatre gènes mitochondriaux et deux gènes nucléaires) comprenant 138 specimens (incluant 98 specimens du groupe A. albivena et 23 specimens du groupe A. stygiata) de 48 espèces de foreurs. Les résultats de ces analyses soutiennent à la fois l’hypothèse de monophylie des deux groupes d’intérêt et le statut d’espèces des taxa nouvellement décris, à l’exception d’A. lusinga qui n’a pas été séquencé. Les analyses phylogénétiques révèlent également l’existence de plusieurs lignées évolutives distinctes dont le statut reste à définir car leur ADN a été extrait de stades larvaires

In this study, we review six morphologically similar species of Sesamia Guenée, 1852 from Africa, including three new species that are described: Sesamia corymbosus Le Ru n. sp., S. schoenoplectus Le Ru n. sp. and S. temberma Le Ru n. sp. These six species belong to a species complex that we hereby define as the Sesamia coniota group. Host plants of four species are recorded: Schoenoplectus corymbosus (Roth ex Roem. & Schult.) J. Raynal. for S. corymbosus and S. schoenoplectus, Echinochloa pyramidalis (Lam.) Hitschc. & Chase, Eriochloa meyeriana (Nees) Pilg., Schoenoplectus corymbosus and Typha latifolia L. for S. jansei, Tams & Bowden, 1953 and Pennisetum sp. for S. temberma. We also conduct molecular phylogenetic analyses on a multimarker (four mitochondrial and two nuclear genes) molecular dataset encompassing 36 specimens (including 32 specimens belonging to the S. coniota group). Molecular analyses allow assessing the phylogenetic relationships of five out of six species of the group Phylogénie and systématique du groupe d’espèces Sesamia coniota (Lepidoptera : Noctuidae : Apameini : Sesamiina) avec la description de trois nouvelles espèces de la région afrotropicale. Dans cette étude, nous passons en revue six espèces du genre Sesamia Guenée, 1852 distribuées en Afrique. Trois nouvelles espèces sont décrites : Sesamia corymbosus Le Ru n. sp., S. schoenoplectus Le Ru n. sp. et S. temberma Le Ru n. sp. Ces six espèces appartiennent à un complexe d’espèces que nous définissons comme le groupe d’espèce Sesamia coniota. Les plantes-hôtes de quatre espèces sont répertoriées : Schoenoplectus corymbosus (Roth ex Roem. & Schult.) J. Raynal. pour S. corymbosus et S. schoenoplectus, Echinochloa pyramidalis (Lam.) Hitschc. & Chase, Eriochloa meyeriana (Nees) Pilg., Schoenoplectus corymbosus et Typha latifolia L. pour S. jansei Tams & Bowden, 1953, et Pennisetum sp. pour S. temberma. Nous avons également réalisé des analyses de reconstruction phylogénétique sur un jeu de données moléculaires multimarqueurs (quatre gènes mitochondriaux et deux gènes nucléaires) comprenant 36 spécimens (dont 32 appartiennent au groupe d’espèce S. coniota). Les analyses moléculaires permettent d’inférer les relations phylogénétiques de cinq des six espèces du groupe

Figs 2F, N, 3D, 7A–E Poecopa mediopuncta Bowden, 1956: 418 –420, fgs 6–9. Poecopa mediopuncta – Poole 1989: 818 (catalogue). Diagnosis Male easily separated from males of other species of the group by the broadly based, stout, strongly curved cornutus, pointed downward at a right angle (Fig. 2N); female easily separated from females of other species of the group by the small sclerotized area at the base of the ductus bursae and the weakly sclerotized antrum (Fig. 3D). Material examined Holotype GHANA: ♂, Gold Coast, Kwadaso, near Kumasi, 25 Jun. 1952, ex larva (in stems of Rottboellia compressa L.), J. Bowden leg. (BMNH 1953/9, Agrotidae genitalia slide 1338). Allotype GHANA: ♀, Gold Coast, mile 19–20 on Kumasi-Mampong Road, 31 Aug. 1951, ex larva (in stems of Setaria megaphylla (Steud) Dur. & Schinz.), J. Bowden leg. (BMNH 1953/11, Agrotidae genitalia slide 1337). Paratype GHANA: 1 ♂, same locality and date as allotype; 12 paratypes of both sexes were recorded in the original description; only one was found in BMNH. Other material CAMEROON: 1 ♂, Southwest Region, Limbe, Wete Wete, 04°04.417' N, 09°01.250' E, 30 m a.s.l., 2 Feb. 2007, ex larva (in stem of Setaria megaphylla (Steud) Dur. & Schinz.), B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G284). Description The descriptions of the external features of the male holotype and female allotype by Bowden (1956) were accurate. The male looks very similar to the female; however, the general colour of the fore wing is a little bit darker in the male than in the female (Fig. 7A–D). WINGSPAN. 20–32 mm (3 ♂♂); 33 mm (1 ♀). MALE AND FEMALE GENITALIA (Figs 2F, N, 3D). The genitalia of both sexes were described by Bowden (1956) with suffcient detail; however, it should be added that the vesica of the aedeagus is hand-shaped with a stout, broadly based, strongly curved cornutus, pointed downward at a right angle. Bionomics Acrapex mediopuncta is a markedly forest species inhabiting open patches of grasses along forest roads. Larvae collected in Ghana were reported from P. purpureum, R. compressa, S. arundinaceum and S. megaphylla (Bowden 1956) (Table 3). The few larvae collected in Cameroon, the Democratic Republic of the Congo and the Republic of the Congo by our group were all from S. megaphylla; unfortunately, no pictures were taken before pupation. Larvae were collected at the bottom of young stems and were always solitary. Typically, plants exhibiting signs of infestation by A. mediopuncta larvae have a curled, brown, central leaf. No pupae were found in the stems, and therefore the borers probably pupate in the soil near exit holes. Distribution Cameroon, the Democratic Republic of the Congo, Ghana and the Republic of the Congo. The records are from Guineo-Congolian rain forests, lowland rain forests and secondary grassland vegetation mosaics (Mosaics #1–3) (White 1983) (Fig. 4), belonging to the Congolian bioregion (Linder et al. 2012) (Fig. 5).

urn:lsid:zoobank.org:act:B727D00A-7E31-4B44-9B33-E6DC82D548B2 Figs 1G–J, 2B, J, 3B Diagnosis Male easily separated from males of other species of the group by the shovel-shaped uncus (at the apex) and the distal part of the aedeagus (grooved-shaped), with the vesica having a basal tuft of needleshaped cornutus, pointed downward (Fig. 2B, J). Female easily separated from females of other species of the group by the very short ductus bursae, with a strongly sclerotised funnel-shaped connection with the ostium; antrum sclerotized, with a large, broad ventral plate, slightly bilobate, widening to the front, the anterior part shaped like a thin lip and more sclerotized than the posterior part, slightly concave (Fig. 3B). Etymology Named after the village of Kafulo in Zambia. Type material Holotype ZAMBIA: ♂, Western Province, Kafulo, 14°08.726' S, 23°27.638' E, 1056 m a.s.l., 20 Mar. 2012, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G595). Paratypes CAMEROON: 1 ♀, Central Region, Sanaga River, 04°22.387' N, 11°15.162' E, 388 m a.s.l., Dec. 2013, ex light trap (MNHN, gen. prep. LERU Bruno/G603). KENYA: 2 ♂♂, 2 ♀♀, Nyanza Province, edge of Ruma Park, 00°36.293' S, 34°16.046' E, 1221 m a.s.l., 14 Nov. 2012, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G511-G513-G529); 1 ♂, Central Province, Ruiru Aukland, 01°05.063' S, 36°55.621' E, 1595 m a.s.l., Jun. 2011 (MNHN). REPUBLIC OF THE CONGO: 1 ♂, Plateau Region, Lefni River, 02°54.501' S, 15°37.776' E, 320 m a.s.l., 13 Apr. 2013, ex light trap (MNHN, gen. prep. LERU Bruno/G584). TANZANIA: 1 ♂, Iringa Region, Lukumburu, 09°40.048' S, 35°16.892' E, 1299 m a.s.l., 17 Apr. 2015, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G799). UGANDA: 2 ♂♂, Western Region, Itojo, 00°50.546' N, 30°13.131' E, 1070 m a.s.l., 22 May 2014, ex light trap (MNHN, gen. prep. LERU Bruno/G714). ZAMBIA: 1 ♂, same date and locality as holotype, ex. light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G178); 1 ♀, same date and locality as holotype, ex light trap (MNHN, gen. prep. LERU Bruno/G165). Description Both sexes look similar; however, general shape of female fore wing more elongated at apex than in male and fore wings paler in females (Fig. 1G–J); antennae bright fuscous dorsally and ochreous ventrally, fliform in female and slightly ciliate in male; fagellum adorned dorsally with black scales, palpus cupreous brown, eyes fuscous. Head and base of thorax brown, thorax becoming gradually fuscous; legs brown-ringed with buff, buff on inner surface; abdomen fuscous, irrorated with buff scales. FORE WING. Ground colour ochreous in both sexes, suffused with fuscous scales, more heavily along veins and costal area, particularly in males; reniform indicated by a few white scales, preceded by some brown scales; longitudinal brown median fascia along lower external margin of cell, ending obliquely at apex; veins below cell adorned with white, fuscous and brown scales; postmedial row of white spots on veins; row of black elongated spots between veins on margin; fringe whitish externally, fuscous suffused with brown internally. Underside of fore wing with ground colour fuscous, densely suffused with brown scales. HIND WING. Ground colour white, veins slightly irrorated, with fuscous scales, costa and apex more heavily suffused with fuscous scales; hind wing of males much more suffused with fuscous scales than in females; fringe white, suffused with fuscous and adorned with narrow fuscous line. Underside of hind wing white, suffused with fuscous scales but much more heavily on costa and apex; veins slightly irrorated, with fuscous scales. WINGSPAN. 18–22 mm (6 ♂♂); 21–25 mm (5 ♀♀). MALE GENITALIA (Fig. 2B, J). Uncus long, widening in distal third, shovel-shaped at apex, tufted with long hairs on upper side. Tegumen with medium-sized rounded penniculi, vinculum pointed, with mediumsized triangular saccus, valves short and broad, cucullus rounded and tufted with medium-sized hairs, coastal margin slightly broadened on inner side and produced into strong, tooth-shaped spine, strongly sclerotized at apex, pointed and slightly curved inwardly; juxta large, plate-like, without sclerotisation. Aedeagus short, slightly curved, with two lateral areas adorned with short setae; hand-shaped vesica with basal tuft of needle-shaped cornutus, pointed downward. FEMALE GENITALIA (Fig. 3B). Corpus bursae elongated ovoid and globular without signa; ductus bursae very short, with strongly sclerotised, funnel-shaped connection with ostium; antrum sclerotized, with large, broad ventral plate, slightly bilobate, widening to the front, anterior part shaped like a thin lip, more sclerotized than posterior part, slightly concave; dorsal plate small, weakly sclerotized. Ovipositor lobes relatively short (2.2 times as long as wide), with pointed apex, dorsal surface bearing numerous short and stout setae. Bionomics Biology unknown. The moths were caught in a light trap in grasslands near marshes. Distribution Cameroon,Kenya,theRepublicoftheCongo,Tanzania,UgandaandZambia.Mothswerefoundinamosaic of lowland rainforest and secondary grassland (Mosaic #11), in a mosaic of Zambezian dry evergreen forest and wetter miombo woodland (Mosaic #21), in a mosaic of East African evergreen bushland and secondaryAcacia wooded grassland (Mosaic #45) and in undiffentiated montane vegetation (Mosaic #19) (White 1983) (Fig. 4), belonging to the Congolian and Zambezian bioregions (Linder et al. 2012) (Fig. 5).

urn:lsid:zoobank.org:act:17B55911-C7C0-4733-B131-D9C0C59FE72A Figs 2C, K, 6A–B Diagnosis Males easily separated from males of other species of the group by the spoon-shaped cucullus and the turn of the hand-shaped vesica being adorned with a large tuft of needle-shaped cornutus (Fig. 2C, K). Etymology Named after the village of Kavumba in Zambia. Type material Holotype ZAMBIA: ♂, Luapula Province, Kavumba, 11°29.074' S, 29°25.757' E, 1193 m a.s.l., 22 Mar. 2012, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G161). Paratypes ZAMBIA: 2 ♂♂, North-Western Province, Rwanko Azi, 12°13.212' S, 25°39.064' E, 1413 m a.s.l., 20 Mar. 2012, ex light trap (MNHN, gen. prep. LERU Bruno/G169-G377). TANZANIA: 1 ♂, Iringa region, Sao Hill, 08°27.421' S, 35°10.036' E, 1845 m a.s.l., 22 Jan. 2012, ex larva (in stem of Hyparrhenia sp.), B. Le Ru leg. (MNHN); 3 ♂♂, same locality, Nov. 2015, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G937). Description Only the male is known (Fig. 6A–B); antennae cupreous brown dorsally and ochreous ventrally, slightly ciliate; fagellum adorned dorsally with white scales, palpus cupreous brown, adorned with white scales, eyes fuscous. Head and base of thorax brown, thorax becoming gradually ochreous; legs brown-ringed with white; abdomen brown irrorated with fuscous scales, extremity of abdomen densely suffused with buff scales. FORE WING. Ground colour dark ochreous, suffused with fuscous and brown scales, more heavily along veins and in costal area; reniform indicated by few white scales, preceded by some brown scales; longitudinal brown median fascia along lower external margin of cell, ending obliquely at apex; veins below cell adorned with white, fuscous and brown scales; postmedial row of white spots on veins; row of black elongated spots between veins on margin; fringe whitish externally, ochreous suffused with brown internally. Underside of fore wing with ground colour brown, suffused with fuscous scales on costa. HIND WING. Uniformly brown; fringe white suffused with fuscous and adorned with narrow fuscous line. Underside of hind wing brown, suffused with fuscous scales. WINGSPAN. 21–23 mm (4 ♂♂). MALE GENITALIA (Fig. 2C, K). Uncus long, widening in distal third, truncated at apex, tufted with long hairs on upper side. Tegumen with medium-sized rounded penniculi, vinculum pointed, with mediumsized triangular saccus, valves short and broad, cucullus spoon-shaped and tufted with medium size hairs, coastal margin slightly broadened on inner side and produced into narrow, straight, long lobe, roundly pointed; juxta oblong, pear-shaped, with long and wide neck, elongate bifd. Aedeagus short, slightly curved, with two lateral areas adorned with short setae; turn of hand-shaped vesica with large tuft of needle-shaped cornutus. Bionomics One larva was collected at the bottom of a stem of a Hyparrhenia sp. growing in grasslands near marshes (Table 3); like many species of Acrapex, A. kavumba sp. nov. is a markedly hygrophilous species. Unfortunately, no pictures were taken before pupation. All the moths were caught in a light trap in grasslands near marshes. Distribution Tanzania and Zambia. The records are from a mosaic of Zambezian dry evergreen forest and wetter miombo woodland (Mosaic #21) (White 1983) (Fig. 4), belonging to the Zambezian bioregion (Linder et al. 2012) (Fig. 5).

urn:lsid:zoobank.org:act:A4657886-D162-4D14-BCD4-46C7A02CC426 Figs 3E, 7F–G Diagnosis Female easily separated from females of other species of the group by the strongly curved and toothshaped ovipositor lobes (Fig. 3E). Etymology Named after the host-plant Miscanthus violaceus (K.Schum.) Pilg. in Uganda. Material examined Holotype UGANDA: ♀, Occidental Province, Kyenjojo, Kazizi, 00°33.865' N, 30°49.117' E, 1251 m a.s.l., 24 May 2014, ex larva (in stems of Miscanthus violaceus), B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G734). Description (Fig. 7F–G) Antennae fuscous dorsally and ochreous ventrally, fliform; fagellum adorned dorsally with black scales, palpus fuscous, eyes black. Head and base of thorax black, thorax ochreous; legs fuscous suffused with white scales, ringed with white; abdomen fuscous, dorsally suffused with grey scales, black ventrally, suffused with grey scales. FORE WING. Ground colour dark ochreous, suffused with fuscous, black and white scales, more heavily along veins and costal area; reniform indicated by few white scales, surrounded by some black scales; irrorated ochreous median area extended on distal side to termen; longitudinal grey median fascia along lower external margin of cell, ending obliquely at apex, adorned with two black elongated spots between veins; veins below cell adorned with grey, white and black scales; fringe grey white, slightly suffused with fuscous. Underside of fore wing with ground colour grey white, suffused with fuscous and some brown scales, more heavily on costa and close to termen. HIND WING. Ground colour white, strongly suffused with fuscous scales; veins slightly irrorated with fuscous scales, costa and apex more heavily suffused with fuscous scales; fringe grey white, suffused with fuscous. Underside of hind wing grey-white, suffused with fuscous scales, but much more heavily on costa and apex; veins slightly irrorated with fuscous scales. WINGSPAN. 22 mm (1 ♀). FEMALE GENITALIA (Fig. 3E). Corpus bursae elongated ovoid and globular, without signa; ductus seminalis from base of bursae; ductus bursae about one third length of corpus bursae, not sclerotised on bursa side, widening and sclerotised on ostial side; antrum narrow, band-like, slightly sclerotised, leaning on back and adorned with very narrow and strongly sclerotised plate divided in two in the middle. Ovipositor lobes relatively short (2 times as long as wide), with dorsal surface bearing numerous short and stout setae, apex of each lobe strongly curved and tooth-shaped. Bionomics Larvae were collected at the bottom of stems of M.violaceus growing in grasslands near marshes (Table 3); as many Acrapex species, A. miscantha sp. nov. is a markedly hygrophilous species. Unfortunately, no pictures were taken before pupation. Distribution Uganda. Only known from the holotype locality in Occidental Province close to Kyenjojo. This species was found in a mosaic of East African evergreen bushland and secondary Acacia wooded grassland (Mosaic #45) (White 1983) (Fig. 4), belonging to the Congolian bioregion (Linder et al. 2012) (Fig. 5).

Bruno Le Ru, Claire Capdevielle-Dulac, Boaz K. Musyoka, Beatrice Pallangyo, Mohamedi Njaku, Onésime Mubenga (2017): Phylogenetic analysis and systematics of the Acrapex unicolora Hampson species complex (Lepidoptera, Noctuidae, Noctuinae, Apameini), with the description of Fve new species from the Afrotropics. European Journal of Taxonomy 270: 1-36, DOI: 10.5852/ejt.2017.270

Figs 2H, P, 3H, 8G–J, 9B Calamistis unicolora Hampson, 1910: 279, pl. 143, fg. 12. Acrapex brunneosa Bethune-Baker, 1911: 517. Busseola hemiphlebia Hampson, 1914: 161. Busseola fuscantis Hampson, 1918: 153. Acrapex simplex Janse, 1939: 359. Acrapex quadrata Berio, 1973: 150, fg. 35. Acrapex brunneosa – Poole 1989: 19 (catalogue). Busseola fuscantis – Poole 1989: 181 (catalogue). Acrapex hemiphlebia – Poole 1989: 20 (recombination, catalogue). Acrapex quadrata – Poole 1989: 20 (catalogue). Acrapex simplex – Poole 1989: 21 (catalogue). Acrapex unicolora – Poole 1989: 21 (recombination, catalogue). Diagnosis Male easily separated from males of other species of the group by the pointed apex of the uncus, the ridge-like, roundly pointed expansion of the coastal margin and by the aedeagus having no vesica (Fig. 2H, P); female easily separated from females of other species of the group by the ductus bursae, which are widening and sclerotised on the ostial side, and by the narrow, band-like, slightly sclerotised antrum (Fig. 3H). Material examined Holotype DEMOCRATIC REPUBLIC OF THE CONGO: ♂, Upper Congo, 1907, A.F.R. Wollaston leg. (BMNH 1907-269, Agrotidae genitalia slide 1458). Other material ANGOLA: 2 ♂♂, N’Dalla Tando, N Angola, 2700 ft, 26 Nov. 1908, Dr W.J. Ansorge leg. (BMNH, Noctuidae genitalia slide 2480). DEMOCRATIC REPUBLIC OF THE CONGO: ♂, holotype of A. quadrata, Sankuru, Dimbelenge, 25 Nov. 1950, Dr M. Fontaine leg. (MRAC, adult; MCSN, genitalia, Berio, E prep N.3753). MALAWI: 1 ♀, Mt Mlanje, Nyasaland, 30 Jun. 1913, S.A. Neave leg. (BMNH 1914-171, Agrotidae genitalia slide No 1345); 5 ♂♂, Mt Mlanje, Nyasaland, 26 Mar. 1913, S.A. Neave leg. (BMNH 1914- 171); 1 ♂, Luchenya River, Mlanje, Nyasaland, 26 Mar. 1913, S.A. Neave leg. (BMNH 1914-171). NIGERIA: ♂, holotype of A. hemiphlebia, Kateregi, 12 Sep. 1910, Scott Macfe leg. (BMNH, 1911-269, Agrotidae genitalia slide No 1460). TANZANIA: 2 ♀♀, Iringa Region, Kifanya, 09°33.443' S, 35°06.246' E, 1675 m a.s.l., 22 Mar. 2007, ex larva (in stem of Andropogon gayanus Kunth), B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/ G365-G366); 2 ♂♂, Iringa Region, Ngongwa, 09°29.665' S, 35°03.137' E, 1662 m a.s.l., 3 Mar. 2008, ex larva (in stem of Andropogon gayanus Kunth), B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/ G364-G474). REPUBLIC OF THE CONGO: 1 ♂, Kouilou Department, Lac Nanga, 04°31.005' S, 12°04.172' E, 35 m a.s.l., 17 Apr. 2013, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G538); 2 ♂♂, 2 ♀♀, Bouenza Department, Kalakundu, 04°22.145' S, 13°40.516' E, 325 m a.s.l., 7 Apr. 2013, ex light trap, B. Le Ru leg. (MNHN, male gen. prep. LERU Bruno/G577, female gen. prep. LERU Bruno/G576). ZAMBIA: 2 ♂♂, 2 ♀♀, Luapula Province, Ngwenya, 12°58.538' S, 28°27.319' E, 1243 m a.s.l., 21 Mar. 2012, ex light trap, B. Le Ru leg. (MNHN, male gen. prep. LERU Bruno/G174, female gen. prep. LERU Bruno/G150); 1 ♀, North-Western Province, Keundwe, 13°06.596' S, 25°21.421' E, 1225 m a.s.l., 19 Mar. 2012, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G158); 1 ♂, 1 ♀, Central Province, Khaembe, 14°33.107' S, 28°19.301' E, 1191 m a.s.l., 15 Mar. 2012, ex light trap, B. Le Ru leg. (MNHN, male gen. prep. LERU Bruno/G170, female gen. prep. LERU Bruno/G171). ZIMBABWE: 1 ♂, Bulawayo Dist., 16 Jan. 1918, ex light trap, A.J.T. Janse leg. (PM, gen. prep. 3370). Redescription (Fig. 8G–J) The sexes look similar; however, the general shape of the female fore wing is more elongated at the apex than in the male; antennae fuscous, fliform in female and slightly ciliate in male; fagellum fuscous, adorned with grey scales, palpus fuscous, suffused with grey scales, eyes fuscous brown. Head and base of thorax brown, thorax dark ochreous; legs brown, suffused with grey scales, ringed with grey; abdomen fuscous, suffused with grey scales. FORE WING. Ground colour dark ochreous, suffused with fuscous and black scales, more heavily along veins, termen and costal area; reniform indicated by few white scales, surrounded by some brown scales; row of black elongated spots on veins in front of reniform; longitudinal brown median fascia along lower external margin of cell, ending obliquely at apex; veins below cell adorned with fuscous brown and white scales; row of black elongated spots between veins on margin; fringe fuscous, slightly suffused with brown. Underside of fore wing with ground colour grey, suffused with fuscous scales, more heavily on costa and close to termen. HIND WING. Ground colour white in female, white ochreous in male, heavily suffused with fuscous scales in male; veins heavily irrorated, with fuscous scales, costa and apex more heavily suffused with fuscous scales; fringe grey, suffused with fuscous. Underside of hind wing grey, suffused with fuscous scales, but much more heavily on costa and apex; veins slightly irrorated, with fuscous scales. WINGSPAN. 20–23 mm (8 ♂♂); 22–28 mm (7 ♀♀). LARVAL L5 INSTAR (Fig. 9B). Length 20–25 mm, width 2.5 mm; head smooth, dark brown, prothoracic shield brown; body with ground colour pink, pinacula and caudal plate dark brown. Young larvae very similar to mature ones. MALE GENITALIA (Fig. 2H, P). Uncus long, widening in distal third, tapering in pointed apex, tufted with long hairs on upper side. Tegumen with medium-sized rounded penniculi, vinculum pointed, with medium-sized triangular saccus, valves short and broad, cucullus rounded and tufted, with mediumsized hairs, coastal margin slightly broadened on inner side and produced into ridge-like expansion, roundly pointed and slightly curved inwardly; large juxta, plate-like, base slightly fattened, without sclerotization, with long and widening neck, slightly bilobate at apex, ending on each side with rounded expansion; aedeagus short, slightly curved. FEMALE GENITALIA (Fig. 3H). Corpus bursae elongated, ovoid, without signa; ductus bursae about onethird length of corpus bursae, not sclerotised on bursa side, widening and sclerotised on ostial side. Antrum narrow, band-like, slightly sclerotised. Ovipositor lobes short (2 times as long as wide), with bluntly pointed apex, dorsal surface bearing numerous short and stout setae. Bionomics Acrapex unicolora is a markedly hygrophilous species of banks of streams, rivers and marshes. Larvae were collected in Tanzania from A. gayanus, Chrysopogon zizanoides (L.) Roberty, Cymbopogon schoenanthus subsp. proximus (Hochst. ex A.Rich.) Maire & Weller, Cymbopogon pospischilii (K. Schum.) C.E.Hubb., Hyparrhenia diplandra (Hack.) Stapf and S. sphacelata (Schumach.) Moss (Table 3). Larvae were collected at the bottom of young stems and were always solitary. Typically, plants exhibiting signs of infestation by A. unicolora larvae have a curled, brown central leaf. No pupae were found in stems and therefore borers probably pupate in the soil near exit holes. Distribution Angola, the Democratic Republic of the Congo, Malawi, Nigeria, the Republic of the Congo, Tanzania, Zambia and Zimbabwe. Known from many localities from sea level to 2147 m a.s.l. Moths were found in a mosaic of lowland rain forest and secondary grassland (Mosaic #11A), a mosaic of Zambezian dry evergreen forest and wetter miombo woodland (Mosaic #21), wetter Zambezian miombo woodland (Mosaic no 25) and undifferentiated montane vegetation (Mosaic #19) (White 1983) (Fig. 4), belonging to the Congolian and to the Zambezian bioregion, respectively (Linder et al. 2012) (Fig. 5). Remarks It is worth highlighting that the records of Acrapex hemiphlebia by Janse (1939) correspond to specimens from a different species that is not yet described and related to Acrapex albivena Hampson, 1910. Phylogenetic and molecular species delimitation analyses Maximum likelihood analyses performed with IQ-TREE yielded a well-supported topology (49 of the 70 nodes supported by BV> 70%; see Fig. 10), especially when considering interspecifc relationships (17 of the corresponding 18 nodes supported by BV> 70%). The only representative of A. mediopuncta (formerly P. mediopuncta) is recovered in a derived position among other members of the genus Acrapex. Members of the A. albivena species group are recovered as sister to the unicolora group (A. albivena, A. salmona, A. sporobola, A. syscia and A. yakoba), with a high support (BV of 96%), while the only representative of the stygiata species group (A. stygiata) is found as sister to both the albivena and unicolora group (BV of 100%). Results of the PTP molecular species delimitation are congruent with the results of the morphological study. Interestingly, PTP analyses highlight the existence of a potential new species refered to as Acrapex sp. SECOG7537 (Fig. 10). This specimen corresponds to a unique larva collected in the Republic of the Congo on Pennisetum unisetum (Nees) Benth., Published as part of Bruno Le Ru, Claire Capdevielle-Dulac, Boaz K. Musyoka, Beatrice Pallangyo, Mohamedi Njaku & Onésime Mubenga, 2017, Phylogenetic analysis and systematics of the Acrapex unicolora Hampson species complex (Lepidoptera, Noctuidae, Noctuinae, Apameini), with the description of Fve new species from the Afrotropics, pp. 1-36 in European Journal of Taxonomy 270 on pages 26-28, DOI: 10.5852/ejt.2017.270, http://zenodo.org/record/889483, {"references":["Hampson G. F. 1910. Catalogue of the Lepidoptera Phalaenae in the Collection of the British Museum (Nat. Hist.). IX. Noctuidae. Taylor and Francis, London.","Hampson G. F. 1914. Descriptions of new genera and species of Noctuidae. Annals and Magazine of Natural History 13: 146 - 175, 197 - 223. http: // dx. doi. org / 10.1080 / 00222931408693462","Janse A. J. T. 1939. The Moths of South Africa. Volume 3. Cymatophoridae, Callidulidae and Noctuidae. E. P. and Commercial Printing Co. Ltd, Durban.","Berio E. 1973. Nuove species e generi di Noctuidae africane e asiatiche e note sinonimiche. Parte II. Annali del Museo Civico di Storia Naturale \" Giacomo Doria \" 79: 126 - 171.","Poole R. W. 1989. Lepidopterorum Catalogus (New Series, Fasc. 118). CRC Press, Boca Raton, FL.","White F. 1983. The vegetation of Africa, a descriptive memoir to accompany the UNESCO / AETFAT / UNSO vegetation map of Africa. UNESCO, Natural Resources Research 20: 1 - 356.","Linder H. P., de Klerk H. M., Born J., Burgess N. D., Fjeldsa J. & Rahbek C. 2012. The partitioning of Africa: statistically defned biogeographical regions in sub-Saharan Africa. Journal of Biogeography 39: 1189 - 1205. http: // dx. doi. org / 10.1111 / j. 1365 - 2699.2012.02728. x"]}

urn:lsid:zoobank.org:act:336CE140-8928-451F-A33F-CC74CBBEF930 Figs 3G, 8E–F, 9A Diagnosis Female easily separated from females of other species of the group by the sclerotized, band-like ventral plate, strongly concave on the front (Fig. 3G). Etymology The species epithet refers to the close similarity of the wing pattern with that of A. mediopuncta (Bowden, 1956). Material examined Holotype UGANDA: ♀, Southern Region, Kayanga Forest, 00°22.027' S, 30°06.722' E, 1447 m a.s.l., 6 Apr. 2006, ex larva (in stem of Setaria megaphylla (Steud) Dur. & Schinz.), B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G69). Paratypes KENYA: 2 ♀♀, Western Province, Kakamega Forest, 00°22.530' N, 34°53.660' E, 1430 m a.s.l., May 2007, ex larva (in stem of Setaria megaphylla), B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G31). UGANDA: 3 ♀♀, same date and locality as holotype, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G32, G770). Description (Fig. 8E–F) Antennae ochreous, fliform; fagellum ochreous, palpus ochreous, eyes black. Head and base of thorax brown, thorax ochreous; legs brown, suffused with ochreous scales, ringed with ochreous; abdomen ochreous, suffused with fuscous scales. FORE WING. Ground colour bright ochreous, suffused with dark ochreous and fuscous scales, more heavily between veins and on costal area; reniform indicated by few white scales, surrounded by some black scales; longitudinal brown median fascia along lower external margin of cell, ending obliquely at apex, adorned with two black elongated spots between veins; row of black elongated spots between veins on margin; fringe ochreous, suffused with brown. Underside of fore wing with ground colour ochreous, heavily suffused with fuscous and brown scales. HIND WING. Ground colour grey white, strongly suffused with fuscous scales, more heavily on costa and apex; veins slightly irrorated, with fuscous scales; fringe grey white, suffused with fuscous. Underside of hind wing grey white, suffused with fuscous scales, but much more heavily on costa and apex; veins slightly irrorated, with fuscous scales. WINGSPAN. 26–32 mm (7 ♀♀). LARVAL L5 INSTAR (Fig. 9A). Length 20–25mm, width 2.5 mm; head smooth, black, prothoracic shield brown; body with ground colour dark pink, pinacula and caudal plate black. Young larvae very similar to mature ones. FEMALE GENITALIA (Fig. 3G). Corpus bursae elongated, ovoid and globular, without signa; ductus seminalis from base of bursae; ductus bursae about less than half length of corpus bursae, not sclerotised on bursa side, widening and slightly sclerotised on ostial side; antrum ovoid, with sclerotized, band-like ventral plate, strongly concave on front; ovipositor lobes relatively short (2 times as long as wide), with bluntly pointed apex, dorsal surface bearing numerous short and stout setae. Bionomics Acrapex simillima sp. nov. is a markedly forest species, inhabiting open patches of grasses along forest roads. Larvae were all collected at the bottom of young stems of S. megaphylla (Table 3) and were always solitary. Typically, plants exhibiting signs of infestation by A. simillima sp. nov. larvae have a curled, brown central leaf. One pupa was found in a stem; however, as in most species of Acrapex, most larvae probably pupate in the soil near exit holes. Distribution Kenya and Uganda. The records are from Guineo-Congolian rain forests (Mosaic #1) (White 1983) (Fig. 4), belonging to the Congolian bioregion (Linder et al. 2012) (Fig. 5).

urn:lsid:zoobank.org:act:5FD4E344-68BA-423B-AF86-347CAD502914 Figs 2D, L, 3C, 6C–F Diagnosis Male easily separated from males of other species of the group by the uncus being shovel-shaped at the apex and by the large, plate-like juxta, with a narrow pyriform base and a long and widening, slightly sclerotised neck (Fig. 2D); female easily separated from females of other species of the group by having the antrum strongly sclerotized, with a large, broad ventral plate, bilobate, widening to the front, anterior part shaped like a feshy lip, the posterior part concave (Fig 3C). Etymology Named after Kiakouama, the technician who collected this species in the Republic of the Congo. Type material Holotype REPUBLIC OF THE CONGO: ♂, Kouilou Department, Lac Nanga, 04°56.090' S, 11°56.713' E, 2 m a.s.l., 17 Apr. 2013, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G843). Paratypes REPUBLIC OF THE CONGO: 4 ♂♂, same date and locality as holotype, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G533-G537-G781); 4 ♀♀, same date and locality as holotype, ex light trap, B. Le Ru leg. (MNHN, gen. prep. LERU Bruno/G536); 1 ♂, Kouilou Province, Lac Loubi, 04°53.573' S, 11°55.535' E, 4 m a.s.l., 16 Apr. 2013, ex light trap, B. Le Ru leg. (MNHN). Description Both sexes look similar; however, the general shape of the female fore wing is more elongated at the apex than in the male and is paler (Fig. 6C–F); antennae bright ochreous dorsally and ochreous ventrally, fliform in female and slightly ciliate in male; fagellum adorned dorsally with grey scales, palpus ochreous grey, eyes fuscous brown. Head and base of thorax bright brown, thorax ochreous; legs ochreous, ringed with grey white; abdomen grey. FORE WING. Ground colour bright ochreous in both sexes, suffused with fuscous and brown scales, more heavily along veins and costal area, particularly in male; reniform indicated by few white scales, surrounded by some brown scales; longitudinal brown median fascia along lower external margin of cell, ending obliquely at apex; veins below cell adorned with white and fuscous scales; row of black elongated spots between veins on margin; fringe grey externally, ochreous suffused with fuscous internally. Underside of fore wing with ground colour ochreous, densely suffused with brown scales. HIND WING. Ground colour pale ochreous in male, more whitish in female; veins slightly irrorated, with fuscous scales, costa and apex more heavily suffused with fuscous scales; hind wing of male much more suffused with fuscous scales than that of female; fringe pale ochreous, suffused with fuscous and adorned with narrow fuscous line. Underside of hind wing pale ochreous in male, more whitish in female, suffused with brown scales but much more heavily on costa and apex; veins slightly irrorated with pale fuscous scales. WINGSPAN. 16–18 mm (4 ♂♂); 20–23 mm (7 ♀♀). MALE GENITALIA (Fig. 2D, K). Uncus long, widening in distal third, shovel-shaped at apex, tufted with long hairs on upper side. Tegumen with medium-sized rounded penniculi, vinculum pointed, with medium-sized triangular saccus, valves short and broad, cucullus rounded and tufted, with medium-sized hairs, coastal margin slightly broadened on the inner side and produced into strong tooth-shaped spine, strongly sclerotized at apex, pointed and curved inwardly; juxta large, plate-like, base pyriform, without sclerotization, with long and widening, slightly sclerotized neck. Aedeagus short, slightly curved, with two lateral areas adorned with short setae; hand-shaped vesica with basal tuft of needle-shaped cornutus, pointed obliquely downward. FEMALE GENITALIA (Fig. 3C). Corpus bursae long and globular, without signa; ductus bursae very short, with strongly sclerotized funnel-shaped connection with ostium; antrum strongly sclerotized, with large, broad ventral plate, bilobate, widening to the front, anterior part shaped like a feshy lip, posterior part concave; dorsal plate small, weakly sclerotized. Ovipositor lobes relatively short (2 times as long as wide), with pointed apex, dorsal surface bearing numerous short and stout setae. Bionomics Biology unknown. The moths were caught in a light trap in grasslands near marshes. Distribution Republic of the Congo. Known from two close localities only in the Kouilou region, south coast of Pointe Noire. Moths were found in a mosaic of lowland rain forest and secondary grassland (Mosaic #11A) (White 1983) (Fig. 4), belonging to the Congolian bioregion (Linder et al. 2012) (Fig. 5).

International audience; Twelve morphologically similar species of Acrapex Hampson 1894, (Lepidoptera, Noctuidae, Noctuinae, Apameini, Sesamiina), from Western, Central and Eastern Africa are reviewed. Eight of these species are new to science and are described: Acrapex akunamatatan. sp. and A. incrassata n. sp. from Kenya; A. gracilis n. sp., A. iringa n. sp., A. lukumbura n. sp. and A. rungwe n. sp. from Tanzania; A. soyema n. sp. from Ethiopia; and A. zoutoi n. sp. from Benin. All 12 species belong to a species complex that we hereby define as the Acrapex apicestriata group. Host-plants for three of the new species are recorded: Setaria incrassata (Hochst.) Hack. for Acrapex incrassata; Cymbopogon pospishilii (K. Schum.) C.E. Hubb. for A. rungwe; and Andropogon perligulatus Stapf. for A. zoutoi. We also conducted molecular phylogenetic analyses (using maximum likelihood and Bayesian inference) on a six gene multimarker molecular dataset (four mitochondrial and two nuclear gene fragments; 4581 nucleotides in length) consisting of 15 Acrapex species (including seven species from the apicestriata group) and four outgroups species from the subtribe Sesamiina (from genera Busseola Thurau 1904, Sciomesa Tams & Bowden 1953, Pirateolea Moyal, Le Ru, Conlong, Cugala, Defabachew, Matama-Kauma, Pallangyo & Van den Berg 2010 and Sesamia Boisduval & Guenee 1852). Both maximum likelihood and Bayesian inference analyses yield a similar and well-supported topology, which supports the monophyly of the apicestriata group.

International audience; Five morphologically similar species of Acrapex Hampson (Lepidoptera, Noctuidae, Noctuinae, Apameini), from sub-Saharan Africa are reviewed, including four new species that are described: Acrapex mondogeneta Le Ru n. sp., A. mubale Le Ru n. sp., A. robe Le Ru n. sp. and A. rubona Le Ru n. sp. These five species belong to a species complex that we hereby define as the Acrapex minima group. Host plants of three species are recorded; Acrapex minima is recorded for the first time on a host plant, Digitaria natalensis Stent; A. mondogeneta on Hyparrhenia hirta (L.) Stapf and A. rubona on Imperata cylindrica (L.) P. Beauv. We also conducted molecular phylogenetics (using both Bayesian inference and maximum likelihood) and molecular species delimitation analyses (Poisson tree processes) on a six gene multi-marker dataset (four mitochondrial and two nuclear gene fragments; 4582 nucleotides in length) of 42 specimens and 22 species, including 23 specimens from the Acrapex minima group. The results of the corresponding analyses support the monophyly of the group and the species status of the newly described taxa.