1. Rapid assessment of the antitetanus immune status of a subject using Dot-ELISA.
- Author
-
Mastroeni P, Leonardi MS, Gazzara D, and Bizzini B
- Subjects
- Diphtheria-Tetanus-Pertussis Vaccine, Drug Combinations immunology, Humans, Tetanus prevention & control, Antibodies, Bacterial analysis, Diphtheria Toxoid immunology, Enzyme-Linked Immunosorbent Assay, Pertussis Vaccine immunology, Tetanus immunology, Tetanus Toxoid immunology
- Abstract
We describe a Dot-ELISA system for the rapid, specific and reliable assessment of a subject's antitetanus immune status. The conditions for Dot-ELISA were selected to give positive reactions only for sera with antitetanus antibody titers equal to or higher than 0.06 I.U./ml, considered to represent protection when using "in vitro" methods. This Dot-ELISA method could advantageously be applied to the monitoring of vaccination campaigns, as well as for assessing the antitetanus immune status of the wounded.
- Published
- 1989
- Full Text
- View/download PDF