10 results on '"Tartaglia, Francesco"'
Search Results
2. Nodular thyroid disease in the elderly: novel molecular approaches for the diagnosis of malignancy
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Sorrenti, Salvatore, Baldini, Enke, Tartaglia, Francesco, Catania, Antonio, Arcieri, Stefano, Pironi, Daniele, Calò, Pier Giorgio, Filippini, Angelo, and Ulisse, Salvatore
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- 2017
- Full Text
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3. Expression and prognostic value of the cell polarity PAR complex members in thyroid cancer
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Tuccilli, Chiara, Baldini, Enke, Arlot Bonnemains, Yannick, Chesnel, Frank, Sorrenti, Salvatore, De Vito, Corrado, D'Armiento, Eleonora, Antonelli, Alessandro, Fallahi, Poupak, Watutantrige, Sara, Tartaglia, Francesco, Barollo, Susi, Mian, Caterina, Arcieri, Stefano, Mascagni, Domenico, Pironi, Daniele, Bononi, Marco, Vergine, Massimo, Monti, Massimo, Filippini, Angelo, Ulisse, Salvatore, WATUTANTRIGE FERNANDO, Sara, Department of Experimental Medicine, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Department of Surgical Sciences, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Department of Internal Medicine, University of Pisa - Università di Pisa, Department of Medical and Surgical Sciences, Università degli Studi di Padova = University of Padua (Unipd), Sapienza University of Rome [C26A14RNFY, C26N145E4T], Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Universita degli Studi di Padova, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
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0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Cell ,Anaplastic thyroid carcinoma, Apico-basal polarity, APKC, Papillary thyroid carcinoma, PAR complex, PARD3, PARD6, Thyroid ,Biology ,Apico-basal polarity ,03 medical and health sciences ,Internal medicine ,Cell polarity ,Gene expression ,medicine ,Anaplastic thyroid carcinoma ,APKC ,Papillary thyroid carcinoma ,PAR complex ,PARD3 ,PARD6 ,Thyroid ,Oncology ,Thyroid cancer ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,Oncogene ,Cancer ,Cell cycle ,medicine.disease ,Molecular medicine ,3. Good health ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Cancer research - Abstract
International audience; Establishment and maintenance of the apical basal cell polarity, required for proper replication, migration, specialized functions and tissue morphogenesis, relies on three evolutionary conserved complexes: PAR, CRUMBS and SCRIBBLE. Loss of cell polarity/cohesiveness (LOP/C) is implicated in cancer progression, and members of the polarity complex have been described as either oncogenes or oncosuppressors. However, no information on their role in thyroid cancer (TC) progression is available. In the present study, we evaluated the gene expression of the PAR complex members aPKCt, PARD3 alpha/beta and PARD6 alpha/beta/gamma in 95 papillary TC (PTC), compared to their normal matched tissues and in 12 anaplastic TC (ATC). The mRNA and protein levels of investigated genes were altered in the majority of PTC and ATC tissues. In PTC, univariate analysis showed that reduced expression of aPKCt, PARD3 beta and PARD6 gamma mRNAs is associated with increased tumor size, and the reduced expression of PARD3 beta mRNA is associated also with recurrences. Multivariate analysis demonstrated that the presence of lymph node metastasis at diagnosis and the reduced expression of PARD3 beta are independent risk factors for recurrences, with hazard ratio, respectively, of 8.21 (P=0.006) and 3.04 (P=0.029). The latter result was confirmed by the Kaplan-Meier analysis, which evidenced the association between decreased PARD3 beta mRNA levels and shorter disease-free interval. In conclusion, we demonstrated that the expression of PAR complex components is deregulated in the majority of PTC and there is a general trend towards their reduction in ATC tissues. Moreover, a prognostic value for the PARD3 beta gene in PTCs is suggested.
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- 2017
4. CTLA-4 and PD-1 Ligand Gene Expression in Epithelial Thyroid Cancers.
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Tuccilli, Chiara, Baldini, Enke, Sorrenti, Salvatore, Catania, Antonio, Antonelli, Alessandro, Fallahi, Poupak, Tartaglia, Francesco, Barollo, Susi, Mian, Caterina, Palmieri, Andrea, Carbotta, Giovanni, Arcieri, Stefano, Pironi, Daniele, Vergine, Massimo, Monti, Massimo, and Ulisse, Salvatore
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T cells ,THYROID cancer ,GENE expression ,LIGANDS (Biochemistry) ,IMMUNE system - Abstract
The dysregulation of PD-1 ligands (PD-L1 and PD-L2) and CTLA-4 ligands (CD80 and CD86) represents a tumor strategy to escape the immune surveillance. Here, the expression of PD-L1, PD-L2, CD80, and CD86 was evaluated at the mRNA level in 94 patients affected by papillary thyroid carcinoma (PTC) and 11 patients affected by anaplastic thyroid carcinoma (ATC). Variations in the mRNAs in PTC patients were then correlated with clinicopathological features. The expression of all genes was deregulated in PTC and ATC tissues compared to normal tissues. In particular, the downregulation of CD80 was observed above all in ATC. In addition, the increased expression of CD80 associated with longer disease-free survival in PTC. Higher expression of PD-L1 associated with the classical histological variant and with the presence of BRAF
V600E mutation in PTC. The increased PD-L2 expression correlated with BRAFV600E mutation and lymph node metastasis, while its lower expression correlated with the follicular PTC variant. The latter was also associated with the CD80 downregulation, which was also related to the absence of lymph node metastasis. In conclusion, we documented the overall dysregulation of PD-1 and CTLA-4 ligands in PTC and ATC tissues and a possible prognostic value for CD80 gene expression in PTC. [ABSTRACT FROM AUTHOR]- Published
- 2018
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5. Expression and Clinical Utility of Transcription Factors Involved in Epithelial–Mesenchymal Transition during Thyroid Cancer Progression.
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Baldini, Enke, Tuccilli, Chiara, Pironi, Daniele, Catania, Antonio, Tartaglia, Francesco, Di Matteo, Filippo Maria, Palumbo, Piergaspare, Arcieri, Stefano, Mascagni, Domenico, Palazzini, Giorgio, Tripodi, Domenico, Maturo, Alessandro, Vergine, Massimo, Tarroni, Danilo, Lori, Eleonora, Ferent, Iulia Catalina, De Vito, Corrado, Fallahi, Poupak, Antonelli, Alessandro, and Censi, Simona
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EPITHELIAL-mesenchymal transition ,TRANSCRIPTION factors ,THYROID cancer ,CANCER invasiveness ,PROGNOSIS - Abstract
The transcription factors involved in epithelial–mesenchymal transition (EMT-TFs) silence the genes expressed in epithelial cells (e.g., E-cadherin) while inducing those typical of mesenchymal cells (e.g., vimentin). The core set of EMT-TFs comprises Zeb1, Zeb2, Snail1, Snail2, and Twist1. To date, information concerning their expression profile and clinical utility during thyroid cancer (TC) progression is still incomplete. We evaluated the EMT-TF, E-cadherin, and vimentin mRNA levels in 95 papillary TC (PTC) and 12 anaplastic TC (ATC) tissues and correlated them with patients' clinicopathological parameters. Afterwards, we corroborated our findings by analyzing the data provided by a case study of the TGCA network. Compared with normal tissues, the expression of E-cadherin was found reduced in PTC and more strongly in ATC, while the vimentin expression did not vary. Among the EMT-TFs analyzed, Twist1 seems to exert a prominent role in EMT, being significantly associated with a number of PTC high-risk clinicopathological features and upregulated in ATC. Nonetheless, in the multivariate analysis, none of the EMT-TFs displayed a prognostic value. These data suggest that TC progression is characterized by an incomplete EMT and that Twist1 may represent a valuable therapeutic target warranting further investigation for the treatment of more aggressive thyroid cancers. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Thyroid C-Cell Hyperplasia in an Adolescent with Neurofibromatosis Type 1.
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Segni, Maria, Massa, Rita, Bonifacio, Vincenzo, Tartaglia, Francesco, Pucarelli, Ida, Marzullo, Antonella, and Pasquino, Anna Maria
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HYPERPLASIA ,THYROID cancer ,NEUROFIBROMATOSIS ,PHAKOMATOSES ,NEUROFIBROMA ,DISEASES in teenagers - Abstract
Background: Subjects with neurofibromatosis type 1 (NF1) show an increased risk of endocrine tumors, especially pheochromocytoma, whereas thyroid C-cell hyperplasia (CCH) and medullary thyroid carcinoma (MTC) are very rare events described only in adult patients. Method: A case of CCH diagnosed in a 14-year-old girl affected with NF1 is reported. Calcitonin serum level after pentagastin was elevated (286 pg/ml). Genetic testing was performed in order to rule out mutations in the RET proto-oncogene. Result: No germline mutation previously reported in MEN2 was detected. Multifocal and bilateral CCH was demonstrated by immunohistochemistry. Conclusion: It is suggested that in such a genetic background of high risk for malignancy, CCH could be considered as an extremely rare condition likely preceding MTC.Copyright © 2002 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
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- 2001
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7. PD-1 Ligand Expression in Epithelial Thyroid Cancers: Potential Clinical Implications.
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Ulisse, Salvatore, Tuccilli, Chiara, Sorrenti, Salvatore, Catania, Antonio, Tartaglia, Francesco, Amabile, Maria Ida, Giacomelli, Laura, Metere, Alessio, Pironi, Daniele, Carbotta, Giovanni, Vergine, Massimo, Monti, Massimo, Baldini, Enke, Antonelli, Alessandro, Fallahi, Poupak, D'Armiento, Eleonora, and Cornacchini, Nicola
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THYROID cancer ,APOPTOSIS ,IMMUNOTHERAPY ,LIGANDS (Biochemistry) ,ONCOGENES - Abstract
The new immunotherapy targeting the programmed cell death 1 (PD-1) receptor and its cognate ligand PD-L1 has renewed hopes of eradicating the most difficult human cancers to treat. Among these, there are the poorly differentiated and anaplastic thyroid cancers, unresponsive to all the therapies currently in use. In the present review we will summarize information regarding the expression of PD-L1 in the different thyroid cancer histotypes, its correlation with clinicopathological features, and its potential prognostic value. Then, we will evaluate the available data indicating the PD-1/PD-L1 axis as a promising target for thyroid cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2019
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8. New perspectives in the diagnosis of thyroid follicular lesions.
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Baldini, Enke, Sorrenti, Salvatore, Tartaglia, Francesco, Catania, Antonio, Palmieri, Andrea, Pironi, Daniele, Filippini, Angelo, and Ulisse, Salvatore
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Thyroid nodules are very common, affecting 19%-67% of the adult population. However, about 10% of them harbor a malignant lesion. Consequently, the first aim in their clinical evaluation is to exclude malignancy. Fine-needle aspiration cytology (FNAC) represents the main diagnostic tool for the evaluation of thyroid nodules. However, FNAC has a main diagnostic limit, namely cellular atypias of indeterminate significance, which require surgical excision and histological examination to differentiate benign from malignant lesions. Histology reports show that approximately 80% of these patients harbor a benign lesion. Therefore, in order to reduce unnecessary thyroidectomy, over the last years, the cytological classification of thyroid nodules has been revised and a number of new instrumental and molecular approaches have been proposed. In the present article, we will attempt to summarize the most recent cytological, molecular and echographic strategies to enhance the diagnostic accuracy of preoperative thyroid follicular lesions. In particular, we will discuss the new cytological classifications from the Bethesda System for Reporting Thyroid Cytopathology (BSRTC), the British Thyroid Association-Royal College of Pathologists (PTA-RCPath) and the new Italian Society for Anatomic Pathology and Cytology (SIAPEC 2014. We will review molecular tests evaluated to ameliorate follicular lesion diagnosis as well as the clinical utility of the new echographic Thyroid Imaging Reporting and Data System (TI-RADS) score. [ABSTRACT FROM AUTHOR]
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- 2017
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9. Deregulated expression of VHL mRNA variants in papillary thyroid cancer.
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Baldini, Enke, Tuccilli, Chiara, Arlot-Bonnemains, Yannick, Chesnel, Frank, Sorrenti, Salvatore, De Vito, Corrado, Catania, Antonio, D'Armiento, Eleonora, Antonelli, Alessandro, Fallahi, Poupak, Watutantrige-Fernando, Sara, Tartaglia, Francesco, Barollo, Susi, Mian, Caterina, Bononi, Marco, Arceri, Stefano, Mascagni, Domenico, Vergine, Massimo, Pironi, Daniele, and Monti, Massimo
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THYROID cancer , *VON Hippel-Lindau disease , *MESSENGER RNA , *TUMOR suppressor genes , *UNIVARIATE analysis , *REGRESSION analysis - Abstract
Recent findings demonstrated that a subset of papillary thyroid cancers (PTCs) is characterized by reduced expression of the von Hippel-Lindau ( VHL ) tumor suppressor gene, and that lowest levels associated with more aggressive PTCs. In the present study, the levels of the two VHL mRNA splicing variants, VHL-213 (V1) and VHL-172 (V2), were measured in a series of 96 PTC and corresponding normal matched tissues by means of quantitative RT-PCR. Variations in the mRNA levels were correlated with patients' clinicopathological parameters and disease-free interval (DFI). The analysis of VHL mRNA in tumor tissues, compared to normal matched tissues, revealed that its expression was either up- or down-regulated in the majority of PTC. In particular, V1 and V2 mRNA levels were altered, respectively, in 78 (81.3%) and 65 (67.7%) out of the 96 PTCs analyzed. A significant positive correlation between the two mRNA variants was observed (p < 0.001). Univariate analysis documented the lack of association between each variant and clinicopathological parameters such as age, tumor size, histology, TNM stage, lymph node metastases, and BRAF mutational status. However, a strong correlation was found between altered V1 or V2 mRNA levels and DFI. Multivariate regression analysis indicated higher V1 mRNA values, along with lymph node metastases at diagnosis, as independent prognostic factors predicting DFI. In conclusion, the data reported demonstrate that VHL gene expression is deregulated in the majority of PTC tissues. Of particular interest is the apparent protective role exerted by VHL transcripts against PTC recurrences. [ABSTRACT FROM AUTHOR]
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- 2017
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10. Surgical treatment of thyroid follicular neoplasms: results of a retrospective analysis of a large clinical series
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Maria Rosa Pelizzo, Piergiorgio Calò, Giancarlo Troncone, Maurizio De Palma, Angela Pezzolla, Giovanni Conzo, Chiara Dobrinja, Giuseppe Signoriello, Marica Grasso, Giuseppe Siciliano, Gian Luca Ansaldo, Lodovico Rosato, Claudio Gambardella, Luciano Pezzullo, Mario Testini, Micaela Piccoli, Nicola Avenia, Celestino Pio Lombardi, Stefano Spiezia, Ernesto Tartaglia, Francesco Tartaglia, Giovanni Docimo, Conzo, Giovanni, Avenia, Nicola, Ansaldo, Gian Luca, Calò, Piergiorgio, De Palma, Maurizio, Dobrinja, Chiara, Docimo, Giovanni, Gambardella, Claudio, Grasso, Marica, Lombardi, Celestino Pio, Pelizzo, Maria Rosa, Pezzolla, Angela, Pezzullo, Luciano, Piccoli, Micaela, Rosato, Lodovico, Siciliano, Giuseppe, Spiezia, Stefano, Tartaglia, Ernesto, Tartaglia, Francesco, Testini, Mario, Troncone, Giancarlo, Signoriello, Giuseppe, Conzo, G., Avenia, N., Ansaldo, G. L., Calo, P., De Palma, M., Dobrinja, C., Docimo, G., Gambardella, C., Grasso, M., Lombardi, C. P., Pelizzo, M. R., Pezzolla, A., Pezzullo, L., Piccoli, M., Rosato, L., Siciliano, G., Spiezia, S., Tartaglia, E., Tartaglia, F., Testini, M., Troncone, G., Signoriello, G., and de Palma, Maurizio
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Male ,Fine needle cytology ,Follicular neoplasm ,Hemithyroidectomy ,Thyroid cancer ,Total thyroidectomy ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Thyroid Gland ,Thyroiditis ,Postoperative Complications ,0302 clinical medicine ,Endocrinology ,Risk Factors ,Retrospective Studie ,Adenocarcinoma, Follicular ,Thyroid Neoplasm ,Adult ,Aged ,Female ,Humans ,Hypoparathyroidism ,Middle Aged ,Retrospective Studies ,Thyroid Neoplasms ,Thyroidectomy ,Treatment Outcome ,Thyroid ,Diabetes and Metabolism ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Human ,Thyroid nodules ,medicine.medical_specialty ,030209 endocrinology & metabolism ,Adenocarcinoma ,Malignancy ,03 medical and health sciences ,medicine ,business.industry ,Risk Factor ,Follicular ,medicine.disease ,Surgery ,Endocrine surgery ,Postoperative Complication ,business - Abstract
The most appropriate surgical management of "follicular neoplasm/suspicious for follicular neoplasm" lesions (FN), considering their low definitive malignancy rate and the limited predictive power of preoperative clinic-diagnostic factors, is still controversial. On behalf of the Italian Association of Endocrine Surgery Units (U.E.C. CLUB), we collected and analyzed the experience of 26 endocrine centers by computerized questionnaire. 1379 patients, surgically treated after a FN diagnosis from January 2012 and December 2103, were evaluated. Histological features, surgical complications, and medium-term outcomes were reported. Total thyroidectomy (TT) was performed in 1055/1379 patients (76.5 %), while hemithyroidectomy (HT) was carried out in 324/1379 cases (23.5 %). Malignancy rate was higher in TT than in HT groups (36.4 vs. 26.2 %), whereas the rates of transient and definitive hypoparathyroidism following TT were higher than after HT. Consensual thyroiditis (16.8 vs. 9.9 %) and patient age (50.9 vs. 47.9 %) also differed between groups. A cytological FN diagnosis was associated to a not negligible malignancy rate (469/1379 patients; 34 %), that was higher in TT than in HT groups. However, a lower morbidity rate was observed in HT, which should be considered the standard of care in solitary lesions in absence of specific risk factors. Malignancy could not be preoperatively assessed and clinical decision-making is still controversial. Further efforts should be spent to more accurately preoperatively classify FN thyroid nodules. The most appropriate surgical management of “follicular neoplasm/suspicious for follicular neoplasm” lesions (FN), considering their low definitive malignancy rate and the limited predictive power of preoperative clinic-diagnostic factors, is still controversial. On behalf of the Italian Association of Endocrine Surgery Units (U.E.C. CLUB), we collected and analyzed the experience of 26 endocrine centers by computerized questionnaire. 1379 patients, surgically treated after a FN diagnosis from January 2012 and December 2103, were evaluated. Histological features, surgical complications, and medium-term outcomes were reported. Total thyroidectomy (TT) was performed in 1055/1379 patients (76.5 %), while hemithyroidectomy (HT) was carried out in 324/1379 cases (23.5 %). Malignancy rate was higher in TT than in HT groups (36.4 vs. 26.2 %), whereas the rates of transient and definitive hypoparathyroidism following TT were higher than after HT. Consensual thyroiditis (16.8 vs. 9.9 %) and patient age (50.9 vs. 47.9 %) also differed between groups. A cytological FN diagnosis was associated to a not negligible malignancy rate (469/1379 patients; 34 %), that was higher in TT than in HT groups. However, a lower morbidity rate was observed in HT, which should be considered the standard of care in solitary lesions in absence of specific risk factors. Malignancy could not be preoperatively assessed and clinical decision-making is still controversial. Further efforts should be spent to more accurately preoperatively classify FN thyroid nodules.
- Published
- 2017
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