1. SPECT-computed tomography in rats with TNBS-induced colitis: A first step toward functional imaging.
- Author
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Marion-Letellier R, Bohn P, Modzelewski R, Vera P, Aziz M, Guérin C, Savoye G, and Savoye-Collet C
- Subjects
- Animals, Blotting, Western, Citrates administration & dosage, Colitis chemically induced, Colon metabolism, Crohn Disease chemically induced, Cyclooxygenase 2 metabolism, Disease Models, Animal, Feasibility Studies, Gallium administration & dosage, Male, Radiopharmaceuticals administration & dosage, Rats, Rats, Sprague-Dawley, Trinitrobenzenesulfonic Acid toxicity, Colitis diagnostic imaging, Colon diagnostic imaging, Crohn Disease diagnostic imaging, Tomography, Emission-Computed, Single-Photon
- Abstract
Aim: To assess the feasibility of SPECT-computed tomography (CT) in rats with trinitrobenzene sulfonic acid (TNBS)-induced acute colitis and confront it with model inflammatory characteristics., Methods: Colitis was induced in Sprague-Dawley rats by intrarectal injection of TNBS ( n = 10) while controls received vehicle ( n = 10). SPECT-CT with intravenous injection of 10 MBq of 67Ga-Citrate was performed at day 2. SPECT-CT criteria were colon wall thickness and maximal wall signal intensity. Laboratory parameters were assessed: colon weight:length ratio, colon cyclooxygenase-2 expression by western blot and histological inflammatory score., Results: Colon weight/length ratio, colon COX-2 expression and histological inflammatory score were significantly higher in the TNBS group than in the control group ( P = 0.0296, P < 0.0001, P = 0.0007 respectively). Pixel max tend to be higher in the TNBS group than in the control group but did not reach statistical significance ( P = 0.0662). Maximal thickness is significantly increased in the TNBS group compared to the control group ( P = 0.0016) while colon diameter is not ( P = 0.1904). Maximal thickness and colon diameter were correlated to colon COX-2 expression ( P = 0.0093, P = 0.009 respectively) while pixel max was not ( P = 0.22). Maximal thickness was significantly increased when inflammation was histologically observed ( P = 0.0043) while pixel max and colon diameter did not ( P = 0.2452, P = 0.3541, respectively)., Conclusion: SPECT-CT is feasible and easily distinguished control from colitic rats., Competing Interests: Conflict-of-interest statement: We declare that we have no financial and personal relationships with other people or organizations that could inappropriately influence our work.
- Published
- 2017
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