Search

In the EU and world-wide, agriculture is in transition. Whilst we just converted conventional farming imprinted by the post-war food demand and heavy agrochemical usage into integrated and sustainable farming with optimized production, we now have to focus on even smarter agricultural management. Enhanced nutrient efficiency and resistance to pests/pathogens combined with a greener footprint will be crucial for future sustainable farming and its wider environment. Future land use must embrace efficient production and utilization of biomass for improved economic, environmental, and social outcomes, as subsumed under the EU Green Deal, including also sites that have so far been considered as marginal and excluded from production. Another frontier is to supply high-quality food and feed to increase the nutrient density of staple crops. In diets of over two-thirds of the world's population, more than one micronutrient (Fe, Zn, I or Se) is lacking. To improve nutritious values of crops, it will be necessary to combine integrated, systems-based approaches of land management with sustainable redevelopment of agriculture, including central ecosystem services, on so far neglected sites: neglected grassland, set aside land, and marginal lands, paying attention to their connectivity with natural areas. Here we need new integrative approaches which allow the application of different instruments to provide us not only with biomass of sufficient quality and quantity in a site specific manner, but also to improve soil ecological services, e.g. soil C sequestration, water quality, habitat and soil resistance to erosion, while keeping fertilization as low as possible. Such instruments may include the application of different forms of high carbon amendments, the application of macro- and microelements to improve crop performance and quality as well as a targeted manipulation of the soil microbiome. Under certain caveats, the potential of such sites can be unlocked by innovative production systems, ready for the sustainable production of crops enriched in micronutrients and providing services within a circular economy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Alfalfa was cultivated in two potted soil series obtained from two sandy soils contaminated by Cu (SM) and metal(loids)/PAH (CD). Shoot production was monitored for 8 weeks. Then, larvae of Spodoptera exigua were reared on alfalfa of both soil series for eight days. A biotest (using Phaseolus vulgaris) was used to assess the soil phytotoxicity. Increasing soil contamination reduced P. vulgaris growth, but alfalfa growth was only reduced on the SM soil series. Exposure to the SM soil was mirrored by shoot Cu and Cr concentrations of alfalfa (respectively, in mg kg -1 DW, Cu and Cr ranged from 11.9 and 0.4 in the CTRL soil to 98.5 and 1.2 in the SM one). Exposure to the CD soil series was mirrored by shoot Zn concentrations (i.e., 48-91.6 mg kg -1 DW). Internal metal(loid) concentrations of S. exigua remained generally steady across both soil series (respectively Cd 0.05-0.16, Cr 0.5-3.3, Cu 5.8-98.5, Ni 0.6-1.6, Pb 0.4-1.3, and Zn 57-337 mg kg -1 DW), and most of the associated transfer factors were lower than 1. Here, due to the excluder phenotype of alfalfa across our TE contamination gradients, S. exigua could cope with high total metal(loid) concentration in both contaminated soils.

Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe. Community structure was studied using denaturing gradient gel electrophoresis (DGGE) fingerprinting of Bacteria, α- and β-Proteobacteria, Actinobacteria and Streptomycetaceae, and sequencing of DGGE bands characteristic of specific treatments. The number of copies of genes involved in ammonia oxidation and denitrification were determined by qPCR. Phytomanagement increased soil microbial biomass at three sites and respiration at the Biogeco site (France). Enzyme activities were consistently higher in treated soils compared to untreated soils at the Biogeco site. At this site, microbial biomass increased from 696 to 2352 mg ATP kg -1 soil, respiration increased from 7.4 to 40.1 mg C-CO 2 kg -1 soil d -1 , and enzyme activities were 2-11-fold higher in treated soils compared to untreated soil. Phytomanagement induced shifts in the bacterial community structure at both, the total community and functional group levels, and generally increased the number of copies of genes involved in the N cycle (nirK, nirS, nosZ, and amoA). The influence of the main soil physico-chemical properties and trace element availability were assessed and eventual site-specific effects elucidated. Overall, our results demonstrate that phytomanagement of TECS influences soil biological activity in the long term., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Biochars are used as amendments to improve soil quality, but their effects on edaphic organisms such as earthworms remain controversial. This study aimed to assess the effects of adding a poultry manure-derived biochar into a contaminated technosol on trace element (TE) (i.e. As, Cd, Cu, Pb, and Zn) bioavailability for two earthworm species, Aporrectodea icterica and Aporrectodea longa. Three components of the bioavailability concept were determined using a pot experiment: (1) total soil TE (potentially reactive) and TE concentrations in the soil pore water (environmental availability), (2) TE concentrations in depurated whole earthworm bodies (environmental bioavailability) and (3) ecophysiological and biochemical effects on earthworms (toxicological bioavailability). Biochar addition increased TE concentrations in the soil pore water respectively from 1.8, 2.7, 9.4, 0.7 and 959 to 6, 6.2, 19.3, 6.9, and 3003 µg L -1 for As, Cd, Cu, Pb and Zn. Biochar addition did not influence TE environmental bioavailability for earthworms, except a decreased As concentration (32.5 to 15.2 µg g -1 ) in A. icterica. This suggests an inter-specific variability in As homeostasis in the Aporrectodea genus. In line with this internal As decrease, the Glutathione-S-transferase (GST) activity decreased by 42% and protein and lipid contents slightly increased (14 and 25%, respectively) in A. icterica tissues. The body weight of both earthworm species decreased for the biochar-amended soil. Environmental TE availability depended on both the biochar addition and the earthworm activity in the contaminated soil, while environmental and toxicological bioavailabilities resulted from the earthworm species, the targeted TE and biochar supply to the soil.

Background and Aims: Rapeseed (Brassica napus L.) is a Cd/Zn-accumulator whereas soil conditioners such as biochars may immobilize trace elements. These potentially complementary soil remediation options were trialed, singly and in combination, in a pot experiment with a metal(loid)-contaminated technosol., Methods: The technosol [total content in mg kg(-1) Zn 6089, Cd 9.4, Cu 110, and Pb 956] was either amended (2% w/w) or not with a poultry manure-derived biochar. Rapeseed was cultivated for both soil treatments during 24 weeks up to harvest under controlled conditions., Results: Biochar incorporation into the technosol promoted the As, Cd, Cu, Mo, Ni, Pb and Zn solubility. It decreased foliar B, Cu and Mo concentrations, and Mo concentration in stems, pericarps and seeds. But, it did not impact neither the biomass of aerial rapeseed parts (except a decrease for seeds), nor their C (except a decrease for stems), seed fatty acid, seed starch and soluble sugar contents, and antioxidant capacity in both leaves and seeds. Biochar amendment increased the phytoextraction by aerial plant parts for K, P, and S, reduced it for N, Ca, B, Mo, Ni and Se, whereas it remained steady for Mg, Zn, Fe, Mn, Cu, Cd and Co., Conclusions: The biochar incorporation into this technosol did not promote Cd, Cu and Zn phytoextraction by rapeseed and its potential oilseed production, but increased the solubility of several metal(loid)s. Here Zn and Cd concentrations in the soil pore water were decreased by rapeseed, showing the feasibility to strip available soil Zn and Cd in combination with seed production., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Foliar ionome, photosystem II activity, and leaf growth parameters of Ranunculus acris L., a potential biomonitor of trace element (TE) contamination and phytoavailability, were assessed using two riverbank soil series. R. acris was cultivated on two potted soil series obtained by mixing a TE (Cd, Cu, Pb, and Zn)-contaminated technosol with either an uncontaminated sandy riverbank soil (A) or a silty clay one slightly contaminated by TE (B). Trace elements concentrations in the soil-pore water and the leaves, leaf dry weight (DW) yield, total leaf area (TLA), specific leaf area (SLA), and photosystem II activity were measured for both soil series after a 50-day growth period. As soil contamination increased, changes in soluble TE concentrations depended on soil texture. Increase in total soil TE did not affect the leaf DW yield, the TLA, the SLA, and the photosystem II activity of R. acris over the 50-day exposure. The foliar ionome did not reflect the total and soluble TE concentrations in both soil series. Foliar ionome of R. acris was only effective to biomonitor total and soluble soil Na concentrations in both soil series and total and soluble soil Mo concentrations in the soil series B.

Medicago sativa was cultivated at a former harbor facility near Bordeaux (France) to phytomanage a soil contaminated by trace elements (TE) and polycyclic aromatic hydrocarbons (PAH). In parallel, a biotest with Phaseolus vulgaris was carried out on potted soils from 18 sub-sites to assess their phytotoxicity. Total soil TE and PAH concentrations, TE concentrations in the soil pore water, the foliar ionome of M. sativa (at the end of the first growth season) and of Populus nigra growing in situ, the root and shoot biomass and the foliar ionome of P. vulgaris were determined. Despite high total soil TE, soluble TE concentrations were generally low, mainly due to alkaline soil pH (7.8-8.6). Shoot dry weight (DW) yield and foliar ionome of P. vulgaris did not reflect the soil contamination, but its root DW yield decreased at highest soil TE and/or PAH concentrations. Foliar ionomes of M. sativa and P. nigra growing in situ were generally similar to the ones at uncontaminated sites. M. sativa contributed to bioavailable TE stripping by shoot removal (in g ha(-1) harvest(-1)): As 0.9, Cd 0.3, Cr 0.4, Cu 16.1, Ni 2.6, Pb 4, and Zn 134. After 1 year, 72 plant species were identified in the plant community across three subsets: (I) plant community developed on bare soil sowed with M. sativa; (II) plant community developed in unharvested plots dominated by grasses; and (III) plant community developed on unsowed bare soil. The shoot DW yield (in mg ha(-1) harvest(-1)) varied from 1.1 (subset I) to 6.9 (subset II). For subset III, the specific richness was the lowest in plots with the highest phytotoxicity for P. vulgaris.

The last few decades have seen the rise of Gentle soil Remediation Options (GRO), which notably include in situ contaminant stabilization ("inactivation") and plant-based (generally termed "phytoremediation") options. For trace element (TE)-contaminated sites, GRO aim to either decrease their labile pool and/or total content in the soil, thereby reducing related pollutant linkages. Much research has been dedicated to the screening and selection of TE-tolerant plant species and genotypes for application in GRO. However, the number of field trials demonstrating successful GRO remains well below the number of studies carried out at a greenhouse level. The move from greenhouse to field conditions requires incorporating agronomical knowledge into the remediation process and the ecological restoration of ecosystem services. This review summarizes agronomic practices against their demonstrated or potential positive effect on GRO performance, including plant selection, soil management practices, crop rotation, short rotation coppice, intercropping/row cropping, planting methods and plant densities, harvest and fertilization management, pest and weed control and irrigation management. Potentially negative effects of GRO, e.g., the introduction of potentially invasive species, are also discussed. Lessons learnt from long-term European field case sites are given for aiding the choice of appropriate management practices and plant species.

During the past decades a number of field trials with gentle remediation options (GRO) have been established on trace element (TE) contaminated sites throughout Europe. Each research group selects different methods to assess the remediation success making it difficult to compare efficacy between various sites and treatments. This study aimed at selecting a minimum risk assessment battery combining chemical and ecotoxicological assays for assessing and comparing the effectiveness of GRO implemented in seven European case studies. Two test batteries were pre-selected; a chemical one for quantifying TE exposure in untreated soils and GRO-managed soils and a biological one for characterizing soil functionality and ecotoxicity. Soil samples from field studies representing one of the main GROs (phytoextraction in Belgium, Sweden, Germany and Switzerland, aided phytoextraction in France, and aided phytostabilization or in situ stabilization/phytoexclusion in Poland, France and Austria) were collected and assessed using the selected test batteries. The best correlations were obtained between NH4NO3-extractable, followed by NaNO3-extractable TE and the ecotoxicological responses. Biometrical parameters and biomarkers of dwarf beans were the most responsive indicators for the soil treatments and changes in soil TE exposures. Plant growth was inhibited at the higher extractable TE concentrations, while plant stress enzyme activities increased with the higher TE extractability. Based on these results, a minimum risk assessment battery to compare/biomonitor the sites phytomanaged by GROs might consist of the NH4NO3 extraction and the bean Plantox test including the stress enzyme activities., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Plants and associated microorganisms are used to remediate anthropogenic metal(loid) contamination of water, soils and sediments. This review focuses on the potential of Arundo donax L. (Giant reed) for alleviating risks due to soils, water, and sediments contaminated by trace elements (TE), with emphasis on its advantages and limits over macrophytes and perennial grasses used for bioenergy and plant-based feedstock. Arundo donax is relevant to phytomanage TE-contaminated matrices, notably in its native area, as it possesses characteristics of large biomass production even under nutrient and abiotic stresses, fast growth rate, TE tolerance and accumulation mainly in below ground plant parts. Cultivating A. donax on contaminated lands and in constructed wetlands can contribute to increase land availability and limit the food vs. plant-based feedstock controversy. To gain more tools for decision-taking and sustainable management,further researches on A. donax should focus on: interactions between roots, TE exposure, and rhizosphere and endophytic microorganisms; biomass response to (a)biotic factors; sustainable agricultural practices on marginal and contaminated land; integration into local, efficient, energy and biomass conversion chains with concern to biomass quality and production; Life-Cycle Assessment including contaminant behavior, as well as environmental, agricultural and socio-economic benefits and drawbacks.

Purpose: A former wood exploitation revealing high Cu and As concentration of the soils served as a case study for assisted phytoextraction., Method: P-spiked Linz-Donawitz (LD) slag was used as a soil additive to improve physico-chemical soil properties and in situ stabilize Cu and other trace metals in a sandy Cu-contaminated soil (630 mg kg⁻¹ soil). The LD slag was incorporated into the contaminated soil to consist four treatments: 0% (T1), 1% (T2), 2% (T3), and 4% (T4). A similar uncontaminated soil was used as a control (CTRL). After a 1-month reaction period, potted soils were used for a 2-week growth experiment with dwarf beans., Results: Soil pH increased with the incorporation rate of LD slag. Similarly the soil electrical conductivity (EC, in millisiemens per centimetre) is ameliorated. Bean plants grown on the untreated soil (T1) showed a high phytotoxicity. All incorporation rates of LD slag increased the root and shoot dry weight yields compared to the T1. The foliar Ca concentration of beans was enhanced for all LD slag-amended soil, while the foliar Mg, K, and P concentrations were not increased. Foliar Cu, Zn, and Cr concentrations of beans decreased with the LD slag incorporation rate., Conclusions: P-spiked LD slag incorporation into polluted soil allow the bean growth and foliar Ca concentration, but also to reduce foliar Cu concentration below its upper critical value avoiding an excessive soil EC and Zn deficiency. This dual effect can be of interest for soil remediation at larger scale.

Contamination of agricultural soils with trace elements (TEs) through municipal and industrial wastes, atmospheric deposition and fertilisers is a matter of great global concern. Since TE accumulation in edible plant parts depends on soil characteristics, plant genotype and agricultural practices, those soil- and plant-specific options that restrict the entry of harmful TEs into the food chain to protect human and animal health are reviewed. Soil options such as in situ stabilisation of TEs in soils, changes in physicochemical parameters, fertiliser management, element interactions and agronomic practices reduce TE uptake by food crops. Furthermore, phytoremediation and solubilisation as alternative techniques to reduce TE concentrations in soils are also discussed. Among plant options, selection of species and cultivars, metabolic processes and microbial transformations in the rhizosphere can potentially affect TE uptake and distribution in plants. For this purpose, genetic variations are exploited to select cultivars with low uptake potential, especially low-cadmium accumulator wheat and rice cultivars. The microbial reduction of elements and transformations in the rhizosphere are other key players in the cycling of TEs that may offer the basis for a wide range of innovative biotechnological processes. It is thus concluded that appropriate combination of soil- and plant-specific options can minimise TE transfer to the food chain., (Copyright © 2011 Society of Chemical Industry.)

Metals can be stabilized by soil amendments that increase metals adsorption or alter their chemical forms. Such treatments may limit the risk related to the contamination through reduction of metal transfer to the food chain (reduction of metal uptake by plants and its availability to soil organisms) and metals migration within the environment. There is a need for experiments comparing various soil amendments available at reasonable amounts under similar environmental conditions. The other question is whether all components of soil environment or soil functions are similarly protected after remediation treatment. We conducted a series of pot studies to test some traditional and novel amendments and their combinations. The treatments were tested for several highly Zn/Cd/Pb contaminated soils. Among traditional amendments composts were the most effective – they ensured plant growth, increased soil microbial activity, reduced Cd in earthworms, reduced Pb bioaccessibility and increased share of unavailable forms of Cd and Pb.

Phytomanagement of trace element-contaminated soils can reduce soil toxicity and restore soil ecological functions, including the soil gas exchange with the atmosphere. We studied the emission rate of the greenhouse gases (GHGs) CO2, CH4, and N2O; the potential CH4 oxidation; denitrification enzyme activity (DEA), and glucose mineralization of a Cu-contaminated soil amended with dolomitic limestone and compost, alone or in combination, after a 2-year phytomanagement with a mixed stand of Populus nigra, Salix viminalis, S. caprea, and Amorpha fruticosa. Soil microbial biomass and microbial community composition after analysis of the phospholipid fatty acids (PLFA) profile were determined. Phytomanagement significantly reduced Cu availability and soil toxicity, increased soil microbial biomass and glucose mineralization capacity, changed the composition of soil microbial communities, and increased the CO2 and N2O emission rates and DEA. Despite such increases, microbial communities were evolving toward less GHG emission per unit of microbial biomass than in untreated soils. Overall, the aided phytostabilization option would allow methanotrophic populations to establish in the remediated soils due to decreased soil toxicity and increased nutrient availability. [ABSTRACT FROM AUTHOR]

Preliminary results from the French ASPITET programme demonstrated that Cd background levels in agricultural soils can vary greatly (0.02--6.9 mg Cd kg{sup}-1{end}) depending on parent material and pedogenic processes (Baize, 1997). However, the total Cd content in soil is often not significantly related to the Cd concentration in edible plant parts. A field case study was undertaken across the southern part of the Yonne district, Burgundy, France. This area has various soil series with either low or high geochemical Cd content in the topsoil. Cd availability in soils sampled at 16 sites belonging to five soil series was investigated using single extractions. In addition, shoots (at stem elongation) and grains (at harvest) of field-grown wheat werecollected at the same sites and analysed for macronutrients and trace elements. Cd concentrations in grain varied from 0.015 to 0.146 mg Cd kg{sup}- 1{end} DM depending on soil characteristics, soil series,and plant mineral composition. Cd grain concentrations did not reflect total Cd content in the surface soil layer; however, they were correlated with Cd extracted by a 0.1 M calcium nitrate unbuffered solution, and to a lesser extent with either soil pH or CEC. These three parameters may be useful guides to predict Cd in wheat grain harvestedin the Yonne district. An inverse relationship was found between Cd and Cu contents in grain. The highest Cd concentrations in wheat grain occurred in plants grown on Aubues soils which had marginal Cu and Zn deficiencies in shoots. In order of Cd accumulation in wheat grain, soil series may be ranked as follows: Domerien < Carixien, Terres Noires < Sols Marron < Aubues. [ABSTRACT FROM AUTHOR]

(Aided) phytostabilisation has been proposed as a suitable technique to decrease the environmental risks associated with metal(loid)-enriched mine tailings. Field scale evaluations are needed for demonstrating their effectiveness in the medium- to long-term. A field trial was implemented in spring 2011 in Cu-rich mine tailings in the NW of Spain. The tailings were amended with composted municipal solid wastes and planted with Salix spp., Populus nigra L. or Agrostis capillaris L. cv. Highland. Plant growth, nutritive status and metal accumulation, and soil physico- and bio-chemical properties, were monitored over three years (four years for plant growth). The total bacterial community, α- and β-Proteobacteria , Actinobacteria and Streptomycetaceae were studied by DGGE of 16s rDNA fragments. Compost amendment improved soil properties such as pH, CEC and fertility, and decreased soil Cu availability, leading to the establishment of a healthy vegetation cover. Both compost-amendment and plant root activity stimulated soil enzyme activities and induced important shifts in the bacterial community structure over time. The woody plant, S. viminalis , and the grassy species, A. capillaris , showed the best results in terms of plant growth and biomass production. The beneficial effects of the phytostabilisation process were maintained at least three years after treatment. [ABSTRACT FROM AUTHOR]