1. Tracheal squamous metaplasia in children with endotracheal intubation or tracheostomy.
- Author
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Knaneh-Monem H, Osterbauer B, and Hochstim C
- Subjects
- Child, Humans, Infant, Infant, Newborn, Intubation, Intratracheal adverse effects, Metaplasia, Retrospective Studies, Trachea surgery, Carcinoma, Squamous Cell surgery, Tracheal Neoplasms surgery, Tracheostomy adverse effects
- Abstract
Objectives: In the present study, tracheal epithelial biopsy samples between intubated children, children with tracheostomy and a control group of non-intubated children are compared with respect to their degree of normal differentiation versus the presence of squamous metaplasia., Methods: Tracheal epithelial biopsies were obtained from intubated neonates undergoing tracheostomy, children with tracheostomy undergoing suprastomal granuloma excision and non-intubated control children undergoing laryngoscopy and bronchoscopy. Paraffin tissue blocks were sectioned at 5 μm thickness and subjected to both routine Hematoxylin and Eosin (H&E) staining and immunostained with the relevant antibodies for markers of epithelial differentiation including B-tubulin, CC10, Muc5ac, P63, keratin5 and keratin14., Results: Squamous metaplasia was seen in 3/3 infants, all intubated and in 3/3 children with tracheostomy tubes in place undergoing excision of suprastomal granuloma. No metaplasia was observed in control tracheal epithelial biopsies in 7/7 non-intubated children., Conclusion: Our results demonstrate a clear association between intubation or tracheostomy and the presence of squamous metaplasia which is not otherwise encountered in control pediatric tracheal biopsies., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2021
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