1. KLU suppresses megasporocyte cell fate through SWR1-mediated activation of WRKY28 expression in Arabidopsis .
- Author
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Zhao L, Cai H, Su Z, Wang L, Huang X, Zhang M, Chen P, Dai X, Zhao H, Palanivelu R, Chen X, and Qin Y
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Cytochrome P-450 Enzyme System genetics, DNA-Binding Proteins genetics, Histones genetics, Histones metabolism, Mutation, Ovule growth & development, Ovule metabolism, Plant Components, Aerial physiology, Plant Roots physiology, RNA, Plant genetics, RNA, Plant metabolism, Transcription Factors genetics, Arabidopsis physiology, Arabidopsis Proteins metabolism, Cytochrome P-450 Enzyme System metabolism, DNA-Binding Proteins metabolism, Gene Expression Regulation, Plant physiology, Transcription Factors metabolism
- Abstract
Germ-line specification is essential for sexual reproduction. In the ovules of most flowering plants, only a single hypodermal cell enlarges and differentiates into a megaspore mother cell (MMC), the founder cell of the female germ-line lineage. The molecular mechanisms restricting MMC specification to a single cell remain elusive. We show that the Arabidopsis transcription factor WRKY28 is exclusively expressed in hypodermal somatic cells surrounding the MMC and is required to repress these cells from acquiring MMC-like cell identity. In this process, the SWR1 chromatin remodeling complex mediates the incorporation of the histone variant H2A.Z at the WRKY28 locus. Moreover, the cytochrome P450 gene KLU , expressed in inner integument primordia, non-cell-autonomously promotes WRKY28 expression through H2A.Z deposition at WRKY28. Taken together, our findings show how somatic cells in ovule primordia cooperatively use chromatin remodeling to restrict germ-line cell specification to a single cell., Competing Interests: Conflict of interest statement: R.P. and reviewer D.Z. are co-editors on a 2017 special issue in Frontiers in Plant Science on Molecular and Cellular Plant Reproduction.
- Published
- 2018
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