Search

Your search keyword '"Shukla, Vishnu"' showing total 6 results
Start Over Author "Shukla, Vishnu" Topic transcriptome
6 results on '"Shukla, Vishnu"'

1. Panicle transcriptome of high-yield mutant indica rice reveals physiological mechanisms and novel candidate regulatory genes for yield under reproductive stage drought stress.

Author

Eragam A, Mohapatra A, Shukla V, Kadumuri RV, George AP, Putta L, Akkareddy S, Chavali S, Vemireddy LR, and Ramireddy E

Subjects
Droughts, Plant Breeding, Genes, Regulator, Stress, Physiological genetics, Transcriptome, Oryza metabolism
Abstract

Background: Reproductive stage drought stress (RDS) is a major global threat to rice production. Due to climate change, water scarcity is becoming an increasingly common phenomenon in major rice-growing areas worldwide. Understanding RDS mechanisms will allow candidate gene identification to generate novel rice genotypes tolerant to RDS., Results: To generate novel rice genotypes that can sustain yield under RDS, we performed gamma-irradiation mediated mutation breeding in the drought stress susceptible mega rice variety, MTU1010. One of the mutant MM11 (MTU1010 derived mutant11) shows consistently increased performance in yield-related traits under field conditions consecutively for four generations. In addition, compared to MTU1010, the yield of MM11 is sustained in prolonged drought imposed during the reproductive stage under field and in pot culture conditions. A comparative emerged panicle transcriptome analysis of the MTU1010 and MM11 suggested metabolic adjustment, enhanced photosynthetic ability, and hormone interplay in regulating yield under drought responses during emerged panicle development. Regulatory network analysis revealed few putative significant transcription factor (TF)-target interactions involved in integrated signalling between panicle development, yield and drought stress., Conclusions: A gamma-irradiate rice mutant MM11 was identified by mutation breeding, and it showed higher potential to sustain yield under reproductive stage drought stress in field and pot culture conditions. Further, a comparative panicle transcriptome revealed significant biological processes and molecular regulators involved in emerged panicle development, yield and drought stress integration. The study extends our understanding of the physiological mechanisms and candidate genes involved in sustaining yield under drought stress., (© 2023. BioMed Central Ltd., part of Springer Nature.)

Published
2023
Full Text
View/download PDF

3. Underpinning wheat physiological and molecular responses to co-occurring iron and 1 phosphate deficiency stress 2

Author

Kaur, Gazaldeep, Shukla, Vishnu, Meena, Varsha, Kumar, Anil, Singh, Jagtar, Kandoth, Kaitheri, Mantri, Shrikant, Raouched, Hatem, Kumar Pandey, Ajay, National Agri-Food Biotechnology Institute, Department of Biotechnology, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
iron, growth, [SDV]Life Sciences [q-bio], Triticum aestivum, food and beverages, nutrient homeostasis, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, transcriptome, phosphate
Abstract

Iron (Fe) and phosphate (P) are essential mineral nutrients for plant growth and development. While it is known that Fe and P pathways interacts within plants however, our understanding of the molecular mechanisms regulating nutrient interaction during plant vegetative and reproductive stages remains largely unknown. Herein, we provide a comprehensive physiological and molecular analysis of hexaploid wheat response to single P/Fe and combined Fe and P deficiency. Our data showed that wheat primary root growth was inhibited in response to –Fe, and remarkably rescued by co-occurring deficiencies of Fe and P. Transcriptome analysis revealed drastic and distinct molecular rearrangements to adapt the single and combined nutrient stress with dominance of Fe responsive cis-regulatory elements. Gene-based clustering and root-specific transcriptome expression analysis identify several important unique components induced in response to combined stress –Fe–P, including UDP glycosyltransferases and cytochrome-P450 and glutathione metabolism. These data are consistent with our metabolome data, which further reveals specific metabolite accumulation in –Fe–P those include amino-isobutyric acid, arabinonic acid and aconitic acid. Finally, at reproductive stage alleviations of the negative effect of Fe was also observed in –Fe–P (i.e. spikelet and grain development). Collectively, the data obtained is essential for designing new strategies to improve resilience of crops to cope with the limited nutrients in soils.

Published
2020

4. Physiological and molecular responses to combinatorial iron and phosphate deficiencies in hexaploid wheat seedlings.

Author

Kaur, Gazaldeep, Shukla, Vishnu, Meena, Varsha, Kumar, Anil, Tyagi, Deepshikha, Singh, Jagtar, Kandoth, Pramod Kaitheri, Mantri, Shrikant, Rouached, Hatem, and Pandey, Ajay Kumar

Subjects
*IRON deficiency, *REARRANGEMENTS (Chemistry), *ROOT growth, *PLANT nutrients, *MOLECULAR interactions, *WHEAT
Abstract

Iron (Fe) and phosphorus (P) are the essential mineral nutrients for plant growth and development. However, the molecular interaction of the Fe and P pathways in crops remained largely obscure. In this study, we provide a comprehensive physiological and molecular analysis of hexaploid wheat response to single (Fe, P) and its combinatorial deficiencies. Our data showed that inhibition of the primary root growth occurs in response to Fe deficiency; however, growth was rescued when combinatorial deficiencies occurred. Analysis of RNAseq revealed that distinct molecular rearrangements during combined deficiencies with predominance for genes related to metabolic pathways and secondary metabolite biosynthesis primarily include genes for UDP-glycosyltransferase, cytochrome-P450s, and glutathione metabolism. Interestingly, the Fe-responsive cis-regulatory elements in the roots in Fe stress conditions were enriched compared to the combined stress. Our metabolome data also revealed the accumulation of distinct metabolites such as amino-isobutyric acid, arabinonic acid, and aconitic acid in the combined stress environment. Overall, these results are essential in developing new strategies to improve the resilience of crops in limited nutrients. • This study attempts to understand the molecular changes occurring during the interaction of Phosphorus (P) and Iron (Fe) in hexaploid wheat roots. • P and Fe show cross-talk as Fe deficiency-induced phenotype that was restored by the withdrawal of P. • The –Fe–P deficiencies show predominance for UDP-glycosyltransferases, cytochrome-450 and glutathione-S-transferases transcripts. • The metabolomic changes identified the importance of amino-isobutyric acid, arabinonic acid and aconitic acid during dual deficiency. • This work provides a comprehensive insight to understand the molecular re-arrangements occurring in wheat roots during Fe and P interaction. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

5. Genome-wide expression analysis id 1 entifies core components during iron starvation in hexaploid wheat

Author

Kaur, Gazaldeep, Shukla, Vishnu, Kumar, Anil, Kaur, Mandeep, Goel, Parul, Singh, Palvinder, Shukla, Anuj, Kaur, Jaspreet, Singh, Jagtar, Mantri, Shrikant, Rouached, Hatem, Pandey, Ajay Kumar, Department of Biotechnology, National Agri-Food Biotechnology Institute, Department of Biotechnology, University Institute of Engineering and Technology, (IUET), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
genome bias, gene expression, Triticum aestivum, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, glutathione metabolism, transcriptome, iron starvation
Abstract

Iron is one of essential micronutrient for all organisms. Its deficiency 33 causes a severe loss in crops yield. Nevertheless, our current understanding on major crops response to Fe deficiency remains limited. Herein, we investigated the effect of Fe deprivation at both transcriptomic and metabolic levels in hexaploid wheat. A genome-wide gene expression reprogramming was observed with a total of 5854 genes showing differential expression in roots of wheat subjected to Fe-starved medium. Subsequent, analysis revealed a predominance of strategy-II mode of Fe uptake, with induced genome bias contribution from the A and B genomes. In general, the predominance of genes encoding for nicotianamine synthase, yellow stripe like transporters, metal transporters, ABC transporters and zinc42 induced facilitator-like protein was noticed. Our transcriptomic data were in agreement with the GC-MS analysis that showed an enhancement of accumulation of various metabolites such as fumarate, malonate, succinate and xylofuranose, which could be linked for enhancing Fe-mobilization. Interestingly, Fe starvation causes a significant temporal increase of glutathione-S-transferase both at transcriptional and enzymatic activity, which indicate the important role of glutathione in the response to Fe starvation in wheat roots. Taken together, our result provides new insight on wheat response to Fe starvation and lays foundation to design strategies to improve Fe nutrition in crops.

Published
2019

6. Integrative analysis of hexaploid wheat roots identifies signature components during iron starvation

Author

Kaur, Gazaldeep, Shukla, Vishnu, Kumar, Anil, Kaur, Mandeep, Goel, Parul, Singh, Palvinder, Shukla, Anuj, Kaur, Jaspreet, Singh, Jagtar, Mantri, Shrikant, Rouached, Hatem, Kumar, Ajay Kumar, National Agri-Food Biotechnology Institute, Department of Biotechnology, University Institute of Engineering and Technology, (IUET), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Equipe Hormones, Nutriments et Développement (HoNuDe) (HONUDE), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
iron starvation, Triticum aestivum, glutathione metabolism, gene expression, genome bias, [SDV]Life Sciences [q-bio], food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, transcriptome
Abstract

Iron is one of essential micronutrient for all organisms. Its deficiency 33 causes a severe loss in crops yield. Nevertheless, our current understanding on major crops response to Fe deficiency remains limited. Herein, we investigated the effect of Fe deprivation at both transcriptomic and metabolic levels in hexaploid wheat. A genome-wide gene expression reprogramming was observed with a total of 5854 genes showing differential expression in roots of wheat subjected to Fe-starved medium. Subsequent, analysis revealed a predominance of strategy-II mode of Fe uptake, with induced genome bias contribution from the A and B genomes. In general, the predominance of genes encoding for nicotianamine synthase, yellow stripe like transporters, metal transporters, ABC transporters and zinc42induced facilitator-like protein was noticed. Our transcriptomic data were in agreement with the GC-MS analysis that showed an enhancement of accumulation of various metabolites such as fumarate, malonate, succinate and xylofuranose, which could be linked for enhancing Fe-mobilization. Interestingly, Fe starvation causes a significant temporal increase of glutathione-S-transferase both at transcriptional and enzymatic activity, which indicate the important role of glutathione in the response to Fe starvation in wheat roots. Taken together, our result provides new insight on wheat response to Fe starvation and lays foundation to design strategies to improve Fe nutrition in crops.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results