Search

Rutaceae is a large family, and the genus‐level classification in the subfamilies or tribes of this family is not unified based on different taxonomic treatments. Until now, phylogenetic relationships of some genera in traditional tribe Ruteae have not been clearly resolved. In this study, seven new complete plastomes of this tribe were sequenced, and a comparative analysis was performed to investigate their plastome characteristics and evolution. In addition, we inferred the phylogenetic relationships of Ruteae based on complete plastome and nuclear ITS data. All plastomes exhibited a typical quadripartite structure and were relatively conserved in their structure and gene arrangement. Their genome sizes ranged from 154,656 bp to 160,677 bp, and the size variation was found to be associated with differences in IR expansion and gene loss. A total of 112 to 114 genes were identified in the genomes, including 78 to 79 protein‐coding genes, 30 tRNA genes, 4 rRNA genes, and 2 pseudogenes. Sequence divergence analysis indicated that non‐coding regions exhibited a higher percentage of variable characters, and nine non‐coding and six coding regions were identified as divergent hotspots. Phylogenetic results based on different datasets showed that this tribe was divided into three reciprocally exclusive groups. The phylogenetic analyses between plastome and nuclear ITS data were partly incongruent with each other. This study provides new insights into plastome evolution of Ruteae as well as Rutaceae. The availability of these plastomes provides useful genomic resources for molecular DNA barcodes and phylogenetically informative markers and deepens our understanding of the phylogeny in Ruteae. [ABSTRACT FROM AUTHOR]

Coptis omeiensis, as an important Chinese medicinal plant, has been endangered because of overharvesting. To better understand and protect this species, we report the first complete chloroplast genome sequence of C. omeiensis. The chloroplast genome was found to be 154,684 bp in length, and GC content was 38.2%, with large and small single copy regions (LSC with 85,099 bp and SSC with 17,239 bp in length) separated by two Inverted repeat regions (IRs with 26,173 bp). Meanwhile, the sequence contained 113 genes, including 79 protein-coding, 30 tRNA, and 4 rRNA genes. The phylogeny of Ranunculaceae based on the complete cp genomes of 27 related taxa was also presented in this study. [ABSTRACT FROM AUTHOR]

Allium ovalifolium var. leuconeurum is an endemic plant in Southwest China. The complete chloroplast genome sequence of A. ovalifolium var. leuconeurum was generated by de novo assembly using high-throughput sequencing. The length of chloroplast genome was 153,635 bp in length, containing a large-single copy region (LSC) of 82,693 bp, a small-single copy region (SSC) of 17,932 bp, and a pair of inverted repeats (IRa and IRb) regions of 26,505 bp. The genome contains 132 genes, including 86 protein-coding genes, 8 rRNA genes, and 38 tRNA genes. Phylogenetic analysis with the reported chloroplast sequences shows that A. ovalifolium var. leuconeurum is more closely related to Allium prattii, both of which belong to the Sect. Anguinum. [ABSTRACT FROM AUTHOR]

The complete chloroplast genome sequence of Chelidonium majus L. has been presented in this article. The complete chloroplast genome is 159,741 bp in length with 38.7% of GC content, containing a large single copy region (LSC) of 87,698 bp, and a small single copy region (SSC) of 18,573 bp, which were separated by a pair of 26,735 bp inverted repeat regions (IRs). The sequence contained 132 unique genes, including 37 tRNA, 8 rRNA, and 87 protein-coding genes. This plastid genome is the first report for the genus Chelidonium and will be useful data for developing markers for further studies on resolving the relationship within the Papaveraceae. [ABSTRACT FROM AUTHOR]