1. Profiling the Fungal Microbiome after Fecal Microbiota Transplantation for Graft-versus-Host Disease:Insights from a Phase 1 Interventional Study
- Author
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Yannouck F. van Lier, Thierry Rolling, Gabriel K. Armijo, Bing Zhai, Nienke J.E. Haverkate, Ellen Meijer, Erfan Nur, Bianca Blom, Jonathan U. Peled, Marcel R.M. van den Brink, Tobias M. Hohl, Mette D. Hazenberg, Kate A. Markey, Hematology, AII - Inflammatory diseases, CCA - Cancer Treatment and quality of life, CCA - Imaging and biomarkers, Hematology laboratory, Graduate School, Clinical Haematology, AII - Cancer immunology, CCA - Cancer biology and immunology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Experimental Immunology, and CCA - Cancer Treatment and Quality of Life more...
- Subjects
Fecal microbiota transplantation ,Transplantation ,Acute graft-versus-host disease ,Mycobiota ,Molecular Medicine ,Immunology and Allergy ,Cell Biology ,Hematology ,Allogeneic hematopoietic cell transplantation - Abstract
Disruption of the intestinal bacterial microbiota is frequently observed in the context of allogeneic hematopoietic cell transplantation (HCT) and is particularly pronounced in patients who develop graft-versus-host disease (GVHD). Donor fecal microbiota transplantation (FMT) restores gut microbial diversity and reduces GVHD in HCT recipients. The composition of the intestinal fungal community in patients with GVHD, and whether fungal taxa are transferred during FMT are currently unknown. We performed a secondary analysis of our clinical trial of FMT in patients with steroid-refractory GVHD with a focus on the mycobiota. We characterized the fecal mycobiota of 17 patients and healthy FMT donors using internal transcribed spacer amplicon sequencing. The donor who provided the majority of FMT material in our study represents an n-of-one study of the intestinal flora over time. In this donor, mycobiota composition fluctuated over time while the bacterial microbiota remained stable over 16 months. Fungal DNA was detected more frequently in baseline stool samples from patients with steroid-refractory GVHD than in patients with steroid-dependent GVHD. We could detect fungal taxa in the majority of samples but did not see evidence of mycobiota transfer from donor to recipient. Our study demonstrates the feasibility of profiling the mycobiota alongside the more traditional bacterial microbiota, establishes the methodology, and provides a first insight into the mycobiota composition of patients with GVHD. more...
- Published
- 2023
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