1. Charge variant analysis of proposed biosimilar to Trastuzumab.
- Author
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Dakshinamurthy P, Mukunda P, Prasad Kodaganti B, Shenoy BR, Natarajan B, Maliwalave A, Halan V, Murugesan S, and Maity S
- Subjects
- Antibody-Dependent Cell Cytotoxicity drug effects, Antibody-Dependent Cell Cytotoxicity immunology, Antineoplastic Agents chemistry, Antineoplastic Agents metabolism, Antineoplastic Agents pharmacology, Biosimilar Pharmaceuticals chemistry, Biosimilar Pharmaceuticals pharmacology, Blotting, Western, Breast Neoplasms immunology, Breast Neoplasms metabolism, Breast Neoplasms pathology, Cell Line, Tumor, Cell Proliferation drug effects, Female, Humans, Killer Cells, Natural metabolism, Protein Binding, Surface Plasmon Resonance, Trastuzumab chemistry, Trastuzumab pharmacology, Biosimilar Pharmaceuticals metabolism, Receptor, ErbB-2 metabolism, Receptors, IgG metabolism, Trastuzumab metabolism
- Abstract
Trastuzumab is a humanized monoclonal antibody (mAb) employed for the treatment of HER2 Positive Breast Cancer. A HER2 overexpressing tumor cell binds to Trastuzumab and attracts immune cells which lead to induction of Antibody Dependent Cellular Cytotoxicity (ADCC) by binding to Fc receptors (CD16a or FcγRIIIa) on an effector cell, such as natural killer (NK) cells. The most commonly expressed receptor on NK cell is CD16a which binds to the Fc portion of Trastuzumab. The ligand-independent HER2-HER3 dimerization is the most potent stimulator of downstream pathways for regulation of cell growth and survival. An attempt has been made in this study to understand the impact of charge heterogeneity on the binding kinetics and potency of the monoclonal antibody. Trastuzumab has a pI range of 8.7-8.9 and is composed of mixture of acidic and basic variants beside the main peak. Ion exchange chromatography was used to isolate the acidic, basic, and main peak fractions from in-house proposed biosimilar to Trastuzumab and their activities were compared to the Innovator Trastuzumab Herclon
® . Data from the mass analysis confirmed the potential modifications in both acidic and basic variant. Binding activity studies performed using Surface Plasmon Resonance (SPR) revealed that acidic variants had lesser binding to HER2 in comparison to the basic variants. Both acidic and basic variant showed no significant changes in their binding to soluble CD16a receptors. In vitro assay studies using a breast cancer cell line (BT-474) confirmed the binding potency of acidic variant to be lesser than basic variant, along with reduced anti-proliferative activity for the acidic variant of Trastuzumab. Overall, these data has provided meaningful insights to the impact of antibody charge variants on in vitro potency and CD16 binding affinity of trastuzumab., (Copyright © 2016 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)- Published
- 2017
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