1. Regular enzyme recovery enhances cellulase production by Trichoderma reesei in fed-batch culture.
- Author
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Wang B, Jiang L, Bai H, Yong Q, and Yu S
- Subjects
- Bioreactors microbiology, Cellulase analysis, Fungal Proteins analysis, Fungal Proteins metabolism, Hydrolysis, Trichoderma enzymology, beta-Glucosidase analysis, beta-Glucosidase metabolism, Batch Cell Culture Techniques instrumentation, Cellulase metabolism, Trichoderma growth & development
- Abstract
Objective: To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals., Results: Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24-62%) and β-glucosidase (22-76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3.5-4.5 g l
-1 ., Conclusion: Enzyme recovery at regular intervals during fed-batch cellulase cultivation could protect the enzyme in the culture broth and enhance the enzyme production when the fungal cell concentration is maintained in a reasonable range.- Published
- 2017
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