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Introduction: The aim of the present study was to evaluate the effect of high fiber diet on lipid profile of obese adolescent girls. Methods: This randomized cross over clinical trial was conducted in southern Iran. A total of 56 patients were randomly assigned into two groups. In the first phase, the experiment group had a high fiber diet while the control group consumed their usual diet for 2 months. Then, both groups returned to their usual diet for a month (clean phase) and after that, the diet was replaced for 2 months again (third phase). Serum LDL, HDL, TG and cholesterol were assessed before the intervention, after first phase, clean phase and third phase. Results: The mean reduction of cholesterol, TG, LDL and HDL were significantly lower in high fiber regimens compared to common diet. Conclusion: The results showed that high fiber dietary can significantly reduce lipid profile of obese patients. Consumption of high fiber diet is recommended in order to reduce the adverse effects of obesity.