6 results on '"Parente, Roberta"'
Search Results
2. Vascular endothelial growth factors and angiopoietins as new players in mastocytosis. (VARRICCHI AUTORE CORRISPONDENTE)
- Author
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Marcella, Simone, Petraroli, Angelica, Braile, Mariantonia, Parente, Roberta, Ferrara, Anne Lise, Galdiero, Maria Rosaria, Modestino, Luca, Cristinziano, Leonardo, Rossi, Francesca Wanda, Varricchi, Gilda, Triggiani, Massimo, de Paulis, Amato, Spadaro, Giuseppe, Loffredo, Stefania, Marcella, Simone, Petraroli, Angelica, Braile, Mariantonia, Parente, Roberta, Ferrara, Anne Lise, Galdiero, Maria Rosaria, Modestino, Luca, Cristinziano, Leonardo, Rossi, Francesca Wanda, Varricchi, Gilda, Triggiani, Massimo, de Paulis, Amato, Spadaro, Giuseppe, and Loffredo, Stefania
- Subjects
Vascular endothelial growth factors ,Mastocytosi ,Tryptase ,Angiopoietin ,Mast cell ,Stem cell factor - Abstract
Mastocytosis is a disorder characterized by the abnormal proliferation and/or accumulation of mast cells in different organs. More than 90% of patients with systemic mastocytosis have a gain-of-function mutation in codon 816 of the KIT receptor on mast cells (MCs). The symptoms of mastocytosis patients are related to the MC-derived mediators that exert local and distant effects. MCs produce angiogenic and lymphangiogenic factors, including vascular endothelial growth factors (VEGFs) and angiopoietins (ANGPTs). Serum concentrations of VEGF-A, VEGF-C, VEGF-D, ANGPT1 and ANGPT2 were determined in 64 mastocytosis patients and 64 healthy controls. Intracellular concentrations and spontaneous release of these mediators were evaluated in the mast cell lines ROSAKIT WT and ROSA KIT D816V and in human lung mast cells (HLMCs). VEGF-A, ANGPT1, ANGPT2 and VEGF-C concentrations were higher in mastocytosis patients compared to controls. The VEGF-A, ANGPT2 and VEGF-C concentrations were correlated with the symptom severity. ANGPT1 concentrations were increased in all patients compared to controls. ANGPT2 levels were correlated with severity of clinical variants and with tryptase levels. VEGF-A, ANGPT1 and VEGF-C did not differ between indolent and advanced mastocytosis. ROSAKIT WT, ROSAKIT D816V and HLMCs contained and spontaneously released VEGFs and ANGPTs. Serum concentrations of VEGFs and ANGPTs are altered in mastocytosis patients.
- Published
- 2021
3. Flow-mediated dilation shows impaired endothelial function in patients with mastocytosis.
- Author
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Bucci, Tommaso, Parente, Roberta, De Feo, Giulia, Cardamone, Chiara, and Triggiani, Massimo
- Abstract
Mastocytosis is a rare disease characterized by clonal proliferation of mast cells (MCs) in different organs. Clinical manifestations of mastocytosis are mostly due to release of mediators from MCs and, in many cases, such as urticaria, flushing, angioedema, and anaphylaxis, are an expression of the biological effects of mediators on endothelial cells. Chronic secretion of mediators in patients with mastocytosis can lead to alteration of endothelial function. We sought to investigate endothelial function in patients with mastocytosis using a noninvasive technique of flow-mediated dilation (FMD). Twenty-five adult patients with indolent and advanced forms of mastocytosis and 20 healthy control subjects were enrolled in the study. Ultrasound assessment of FMD was performed by measuring changes in the diameter of the brachial artery after 5 minutes of arterial occlusion. Changes in FMD were correlated with clinical parameters and serum tryptase levels. Patients with mastocytosis had lower FMD compared with healthy control subjects (P <.001). Advanced and smoldering forms showed a lower FMD compared with indolent forms (P <.001). FMD inversely correlated with age and serum tryptase levels and directly with median arterial pressure and recurrent flushing episodes. No correlation was found between FMD and osteoporosis, recurrent anaphylaxis, presence of skin lesions, and long-term antihistamine treatment. Endothelial dysfunction, as demonstrated by FMD reduction, is detectable in patients with mastocytosis and is more severe in patients with high tryptase levels and advanced disease. Endothelial function appears to be negatively influenced by MC proliferation rather than by the severity of mediator-related symptoms. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
4. Altered Metabolism of Phospholipases, Diacylglycerols, Endocannabinoids, and -Acylethanolamines in Patients with Mastocytosis.
- Author
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Ferrara, Anne Lise, Piscitelli, Fabiana, Petraroli, Angelica, Parente, Roberta, Galdiero, Maria Rosaria, Varricchi, Gilda, Marone, Giancarlo, Triggiani, Massimo, Di Marzo, Vincenzo, and Loffredo, Stefania
- Subjects
MAST cell disease ,PHOSPHOLIPASES ,TRYPTASE ,DIGLYCERIDES ,MAST cells ,METABOLISM - Abstract
Background: Mastocytosis is a condition characterized by the expansion and accumulation of mast cells (MCs) in various organs. The symptoms are related to the increased release of MC-derived mediators that exert local and distant effects. MCs are a source and target of phospholipase enzymes (PLs), which catalyze the cleavage of membrane phospholipids releasing lipid mediators (e.g., diacylglycerols (DAGs) and the endocannabinoid (EC) 2-arachidonoylglycerol (2-AG)). To date, there are no data on the role of these lipid mediators in mastocytosis. Here, we analyzed plasma levels of PLA2, PLC, DAG, ECs, and EC-related N-acylethanolamines in patients with mastocytosis.Methods: In 23 patients with mastocytosis and 23 healthy individuals, we measured plasma PLA2 and PLC activities, DAG, 2-AG, anandamide (AEA), palmitoylethanolamide (PEA), and oleoylethanolamide (OEA).Results: Plasma PLA2 and PLC activities were increased in mastocytosis patients compared to controls. Concentrations of DAG (18:1 20:4 and 18:0 20:4), two second messengers produced by PLC, were higher in mastocytosis compared to controls, whereas the concentrations of their metabolite, 2-AG, were not altered. AEA was decreased in mastocytosis patients compared to controls; by contrast, AEA congener, PEA, was increased. PLA2 and PLC activities were increased only in patients with mediator-related symptoms. Moreover, PLC activity was positively correlated with disease severity and tryptase concentrations. By contrast, AEA was negatively correlated with tryptase concentrations.Conclusions: PLs and some lipid mediators are altered in patients with mastocytosis. Our results may pave the way for investigating the functions of these mediators in the pathophysiology of mastocytosis and provide new potential biomarkers and therapeutic targets. [ABSTRACT FROM AUTHOR]- Published
- 2019
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- View/download PDF
5. Thymic stromal lymphopoietin (TSLP) is a substrate for tryptase in patients with mastocytosis.
- Author
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Marcella, Simone, Petraroli, Angelica, Canè, Luisa, Ferrara, Anne Lise, Poto, Remo, Parente, Roberta, Palestra, Francesco, Cristinziano, Leonardo, Modestino, Luca, Galdiero, Maria Rosaria, Monti, Maria, Marone, Gianni, Triggiani, Massimo, Varricchi, Gilda, and Loffredo, Stefania
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THYMIC stromal lymphopoietin , *TRYPTASE , *MAST cell disease , *MAST cells , *INVERSE relationships (Mathematics) - Abstract
• Mastocytosis is a clonal disorder of mast cells. • TSLP is a cytokine involved in allergic disorders and cancer. • Mast cell-derived tryptase is increased in mastocytosis. • TSLP is a substrate for human mast cell tryptase. • TSLP-tryptase is a novel loop involved in mastocytosis. Mastocytosis is a heterogeneous disease associated to uncontrolled proliferation and increased density of mast cells in different organs. This clonal disorder is related to gain-of-function pathogenic variants of the c-kit gene that encodes for KIT (CD117) expressed on mast cell membrane. Thymic stromal lymphopoietin (TSLP) is a pleiotropic cytokine, which plays a key role in allergic disorders and several cancers. TSLP is a survival and activating factor for human mast cells through the engagement of the TSLP receptor. Activated human mast cells release several preformed mediators, including tryptase. Increased mast cell-derived tryptase is a diagnostic biomarker of mastocytosis. In this study, we found that in these patients serum concentrations of TSLP were lower than healthy donors. There was an inverse correlation between TSLP and tryptase concentrations in mastocytosis. Incubation of human recombinant TSLP with sera from patients with mastocytosis, containing increasing concentrations of tryptase, concentration-dependently decreased TSLP immunoreactivity. Similarly, recombinant β-tryptase reduced the immunoreactivity of recombinant TSLP, inducing the formation of a cleavage product of approximately 10 kDa. Collectively, these results indicate that TSLP is a substrate for human mast cell tryptase and highlight a novel loop involving these mediators in mastocytosis. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
6. Altered metabolism of phospholipases, diacylglycerols, endocannabinoids, and N-Acylethanolamines in patients with mastocytosis
- Author
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Anne Lise Ferrara, Giancarlo Marone, Massimo Triggiani, Roberta Parente, Fabiana Piscitelli, Angelica Petraroli, Maria Rosaria Galdiero, Gilda Varricchi, Vincenzo Di Marzo, Stefania Loffredo, Ferrara, Anne Lise, Piscitelli, Fabiana, Petraroli, Angelica, Parente, Roberta, Galdiero, Maria Rosaria, Varricchi, Gilda, Marone, Giancarlo, Triggiani, Massimo, Di Marzo, Vincenzo, and Loffredo, Stefania
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lcsh:Immunologic diseases. Allergy ,Adult ,Male ,medicine.medical_specialty ,Article Subject ,Polyunsaturated Alkamides ,Immunology ,Tryptase ,Arachidonic Acids ,Phospholipase ,Diglycerides ,03 medical and health sciences ,chemistry.chemical_compound ,Oleoylethanolamide ,0302 clinical medicine ,Internal medicine ,medicine ,Immunology and Allergy ,Humans ,endocannabinoids ,030304 developmental biology ,Aged ,0303 health sciences ,Palmitoylethanolamide ,biology ,Chemistry ,General Medicine ,Anandamide ,Lipid signaling ,Middle Aged ,Endocannabinoid system ,Phospholipases A2 ,Endocrinology ,Ethanolamines ,Biomarkers ,Endocannabinoids ,Female ,Mastocytosis ,Tryptases ,Type C Phospholipases ,Second messenger system ,biology.protein ,lipids (amino acids, peptides, and proteins) ,lcsh:RC581-607 ,030215 immunology - Abstract
Background. Mastocytosis is a condition characterized by the expansion and accumulation of mast cells (MCs) in various organs. The symptoms are related to the increased release of MC-derived mediators that exert local and distant effects. MCs are a source and target of phospholipase enzymes (PLs), which catalyze the cleavage of membrane phospholipids releasing lipid mediators (e.g., diacylglycerols (DAGs) and the endocannabinoid (EC) 2-arachidonoylglycerol (2-AG)). To date, there are no data on the role of these lipid mediators in mastocytosis. Here, we analyzed plasma levels of PLA2, PLC, DAG, ECs, and EC-related N-acylethanolamines in patients with mastocytosis. Methods. In 23 patients with mastocytosis and 23 healthy individuals, we measured plasma PLA2 and PLC activities, DAG, 2-AG, anandamide (AEA), palmitoylethanolamide (PEA), and oleoylethanolamide (OEA). Results. Plasma PLA2 and PLC activities were increased in mastocytosis patients compared to controls. Concentrations of DAG (18:1 20:4 and 18:0 20:4), two second messengers produced by PLC, were higher in mastocytosis compared to controls, whereas the concentrations of their metabolite, 2-AG, were not altered. AEA was decreased in mastocytosis patients compared to controls; by contrast, AEA congener, PEA, was increased. PLA2 and PLC activities were increased only in patients with mediator-related symptoms. Moreover, PLC activity was positively correlated with disease severity and tryptase concentrations. By contrast, AEA was negatively correlated with tryptase concentrations. Conclusions. PLs and some lipid mediators are altered in patients with mastocytosis. Our results may pave the way for investigating the functions of these mediators in the pathophysiology of mastocytosis and provide new potential biomarkers and therapeutic targets.
- Published
- 2019
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