1. Fucoidan protects against lipopolysaccharide-induced rat neuronal damage and inhibits the production of proinflammatory mediators in primary microglia.
- Author
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Cui YQ, Jia YJ, Zhang T, Zhang QB, and Wang XM
- Subjects
- Analysis of Variance, Animals, Apomorphine pharmacology, Brain Injuries complications, CD11b Antigen metabolism, Disease Models, Animal, Dopamine Agonists pharmacology, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Male, Mental Disorders drug therapy, Mental Disorders etiology, Microglia drug effects, Rats, Rats, Sprague-Dawley, Reactive Oxygen Species metabolism, Stereotyped Behavior drug effects, Stereotyped Behavior physiology, Tumor Necrosis Factor-alpha genetics, Tyrosine 3-Monooxygenase metabolism, Brain Injuries chemically induced, Brain Injuries prevention & control, Lipopolysaccharides toxicity, Microglia metabolism, Neuroprotective Agents therapeutic use, Polysaccharides therapeutic use, Tumor Necrosis Factor-alpha metabolism
- Abstract
Background: Fucoidan, a sulfated polysaccharide extracted from brown algae, possesses potent antiinflammatory effects., Aims: To examine the effect of fucoidan treatment on inflammation-mediated dopaminergic neuronal damage and its potential mechanisms., Methods: Microglial activation and injury of dopaminergic neurons were induced by intranigral injection of lipopolysaccharide (LPS), and the effects of fucoidan treatment on animal behavior, microglial activation and survival ratio of dopaminergic neurons were investigated. We further observed the efficacy of fucoidan on tumor necrosis factor-alpha (TNF-α) and the production of reactive oxygen species (ROS) in LPS-activated primary microglia., Results: Fucoidan significantly improved the behavioral manifestation, prevented the loss of dopaminergic neurons and inhibited the deleterious activation of microglia in the substantia nigra pars compacta of LPS-treated rats. Further in vitro experiments indicated that the excessive production of TNF-α and ROS in LPS-induced primary microglia were significantly inhibited by fucoidan administration., Conclusion: This is the first study to demonstrate that fucoidan possesses neuroprotective effects on injured dopaminergic neurons in a LPS-induced animal model of Parkinson's disease. The mechanisms underlying these effects may include its potent down-regulation of intracellular ROS and subsequent proinflammatory cytokine release in LPS-activated microglia., (© 2012 Blackwell Publishing Ltd.)
- Published
- 2012
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