23 results on '"Kasuga, Masato"'
Search Results
2. Association of TCF7L2 polymorphisms with susceptibility to type 2 diabetes in 4,087 Japanese subjects
- Author
-
Miyake, Kazuaki, Horikawa, Yukio, Hara, Kazuo, Yasuda, Kazuki, Osawa, Haruhiko, Furuta, Hiroto, Hirota, Yushi, Yamagata, Kazuya, Hinokio, Yoshinori, Oka, Yoshitomo, Iwasaki, Naoko, Iwamoto, Yasuhiko, Yamada, Yuichiro, Seino, Yutaka, Maegawa, Hiroshi, Kashiwagi, Atsunori, Yamamoto, Ken, Tokunaga, Katsushi, Takeda, Jun, Makino, Hideichi, Nanjo, Kishio, Kadowaki, Takashi, and Kasuga, Masato
- Published
- 2008
- Full Text
- View/download PDF
3. Association of insulin treatment with gastric residue during an esophagogastroduodenoscopy.
- Author
-
Kobori, Toshiko, Onishi, Yukiko, Iwamoto, Masahiko, Kubota, Tetsuya, Kikuchi, Takako, Tahara, Tazu, Takao, Toshiko, Fujiwara, Hiroaki, Yoshida, Yoko, and Kasuga, Masato
- Subjects
INSULIN therapy ,DIGESTIVE system endoscopic surgery ,GLYCOSYLATED hemoglobin ,PROPORTIONAL hazards models ,TYPE 2 diabetes ,GLYCEMIC control - Abstract
The purpose of this study was to investigate the association of glycemic control and diabetes treatment to gastric residue observed during an esophagogastroduodenoscopy. Among 6,592 individuals who had esophagogastroduodenoscopy at our clinic between 2003 and 2019, we retrospectively and longitudinally identified those who had gastric residue during an esophagogastroduodenoscopy. Other data collected were age, sex, diagnosis of diabetes, glycated hemoglobin and diabetes medication. Cox proportional hazards models were used to assess the association of these data with the occurrence of gastric residue. To the best of our knowledge, this is the first retrospective cohort study finding that undergoing insulin treatment is a risk factor for gastric residue independent of age, sex and diabetes or glycated hemoglobin. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
4. Synergistic association of the copper/zinc ratio under inflammatory conditions with diabetic kidney disease in patients with type 2 diabetes: The Asahi Diabetes Complications Study.
- Author
-
Takao, Toshiko, Yanagisawa, Hiroyuki, Suka, Machi, Yoshida, Yoko, Onishi, Yukiko, Tahara, Tazu, Kikuchi, Takako, Kushiyama, Akifumi, Anai, Motonobu, Takahashi, Kazuyuki, Wakabayashi Sugawa, Sayaka, Yamazaki, Hiroki, Kawazu, Shoji, Iwamoto, Yasuhiko, Noda, Mitsuhiko, and Kasuga, Masato
- Subjects
DIABETES complications ,TYPE 2 diabetes ,CYSTATIN C ,DIABETIC nephropathies ,GLOMERULAR filtration rate ,COPPER ,ZINC - Abstract
Aims/Introduction: We aimed to study the relationships among the copper (Cu)/zinc (Zn) ratio, inflammatory biomarkers, and the prevalence of diabetic kidney disease (DKD) in patients with type 2 diabetes. Materials and Methods: A cross‐sectional study was performed on 651 patients with type 2 diabetes. DKD was defined as a urinary albumin‐to‐creatinine ratio of ≥30 mg/g creatinine and/or an estimated glomerular filtration rate using cystatin C of < 60 mL/min/1.73 m2. Areas under the curves (AUCs), cutoff values, and thresholds for detecting DKD were determined for the Cu/Zn ratio, soluble tumor necrosis factor‐α receptor 1 (sTNFαR1), and high‐sensitivity C‐reactive protein (hsCRP). Patients were categorized by each cutoff value of sTNFαR1 and the Cu/Zn ratio. Odds ratios (ORs) and biological interactions for the prevalence of DKD were determined. Results: DKD was identified in 220 patients. AUC/optimal cutoff values were 0.777/1300 pg/mL for sTNFαR1, 0.603/1.1648 for the Cu/Zn ratio, and 0.582/305 ng/mL for hsCRP. The ORs for DKD were higher, but not significantly, in the sTNFαR1 < 1300 and Cu/Zn ≥ 1.1648 group, significantly higher in the sTNFαR1 ≥ 1300 and Cu/Zn < 1.1648 group (P < 0.0001), and further synergistically elevated in the sTNFαR1 ≥ 1300 and Cu/Zn ≥ 1.1648 group (P < 0.0001) compared with the sTNFαR1 < 1300 and Cu/Zn < 1.1648 group after multivariable adjustment. Levels of sTNFαR1 were significantly higher in the sTNFαR1 ≥ 1300 and Cu/Zn ≥ 1.1648 group than in the sTNFαR1 ≥ 1300 and Cu/Zn < 1.1648 group (P = 0.0006). Conclusions: Under an inflammatory initiation signal of elevated serum sTNFαR1 levels, an increase in the Cu/Zn ratio may further exacerbate inflammation and is synergistically associated with a high prevalence of DKD in patients with type 2 diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. Thresholds for postprandial hyperglycemia and hypertriglyceridemia associated with increased mortality risk in type 2 diabetes patients: A real‐world longitudinal study.
- Author
-
Takao, Toshiko, Suka, Machi, Yanagisawa, Hiroyuki, and Kasuga, Masato
- Subjects
TYPE 2 diabetes ,PEOPLE with diabetes ,HYPERGLYCEMIA ,HYPERTRIGLYCERIDEMIA ,BLOOD sugar - Abstract
Aims/Introduction: To identify thresholds for postprandial hyperglycemia and hypertriglyceridemia predictive of all‐cause mortality in patients with type 2 diabetes. Materials and Methods: A total of 1,928 patients with type 2 diabetes visited our clinic for the first time from 1995 to 1999 and were followed up for ≥1 year. During the first year, 2‐h post‐breakfast blood glucose (2h‐BG) levels were measured in 1,122 patients (BG cohort) and postprandial serum triglyceride (ppTG) levels were measured in 1,826 patients (TG cohort). Patients were retrospectively followed until 2017 and administered questionnaires. Associations between 2h‐BG and ppTG levels and mortality risk were assessed by the multivariate Cox regression analysis. Results: Over of 17,429 person‐years, 162 deaths occurred in the BG cohort, and over 28,026 person‐years, 253 deaths occurred in the TG cohort. Hazard ratios (HRs) with 95% confidence intervals for all‐cause mortality per 1‐standard deviation increases in 2h‐BG and ppTG were 1.34 (1.08–1.67) and 1.24 (1.06–1.45), respectively. HRs showed increasing trends across quintiles of 2h‐BG (P = 0.034) and ppTG (P = 0.007). The HR was significantly elevated (2.37, 1.26–4.47) in the fifth quintile of 2h‐BG (≥13.8 mmol/L) compared with the first quintile (<7.0 mmol/L; P = 0.008). The HR was also significantly elevated (1.63, 1.03–2.60) in the fifth quintile of ppTG (≥2.30 mmol/L) compared with the first quintile (<0.91 mmol/L; P = 0.038). Conclusions: Postprandial hyperglycemia and hypertriglyceridemia were associated with all‐cause mortality in patients with type 2 diabetes. We propose thresholds of 13.8 mmol/L 2h‐BG and 2.30 mmol/L ppTG to identify patients at increased risk of mortality. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
6. Combined effect of diabetic retinopathy and diabetic kidney disease on all‐cause, cancer, vascular and non‐cancer non‐vascular mortality in patients with type 2 diabetes: A real‐world longitudinal study.
- Author
-
Takao, Toshiko, Suka, Machi, Yanagisawa, Hiroyuki, and Kasuga, Masato
- Subjects
DIABETIC nephropathies ,DIABETIC retinopathy ,TYPE 2 diabetes ,CANCER-related mortality ,MORTALITY - Abstract
Aims/Introduction: We assessed the relationship between diabetic retinopathy (DR) and/or diabetic kidney disease (DKD) according to their severity and all‐cause, cancer, vascular and non‐cancer non‐vascular mortality in real‐world patients with type 2 diabetes. Materials and Methods: A total of 1,902 patients with type 2 diabetes were enrolled from 1995 to 1999 and followed to 2017. At baseline, DR was diagnosed in 374 patients, DKD in 529, vision‐threatening DR in 123 and advanced DKD in 287. Patients were classified by the status of DR and DKD. Multivariate Cox regression analysis was carried out. Results: There were 266 deaths during a median follow‐up period of 18.6 years. Among these, 92 were from cancer, 78 were from vascular causes and 82 were from non‐cancer non‐vascular causes. DR and/or DKD predicted all‐cause, vascular and non‐cancer non‐vascular mortality, but not cancer mortality. Similarly, vision‐threatening DR and/or advanced DKD predicted all‐cause, vascular and non‐cancer non‐vascular mortality, but not cancer mortality. Hazard ratios for all‐cause, vascular and non‐cancer non‐vascular mortality were highest in the DR(+)DKD(+) group, and higher in the DR(−)DKD(+) and the DR(+)DKD(−) groups than in the DR(−)DKD(−) group. The results for vision‐threatening DR and advanced DKD were similar. The interaction for non‐cancer non‐vascular mortality, but not all‐cause and vascular mortality, between DR and DKD and between vision‐threatening DR and advanced DKD might be significant. Conclusions: DR and DKD may be jointly and independently associated with all‐cause, vascular and non‐cancer non‐vascular mortality, but not cancer mortality, according to their severity in real‐world patients with type 2 diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
7. Effect of postprandial hyperglycemia at clinic visits on the incidence of retinopathy in patients with type 2 diabetes: An analysis using real‐world long‐term follow‐up data.
- Author
-
Takao, Toshiko, Takahashi, Kazuyuki, Yoshida, Yoko, Kushiyama, Akifumi, Onishi, Yukiko, Tahara, Tazu, Shimmei, Asuka, Kikuchi, Takako, Suka, Machi, Yanagisawa, Hiroyuki, Iwamoto, Yasuhiko, and Kasuga, Masato
- Subjects
DIABETIC retinopathy ,TYPE 2 diabetes ,HYPERGLYCEMIA ,GLYCOSYLATED hemoglobin ,BLOOD sugar ,MIDDLE-aged persons - Abstract
Aims/Introduction: There is little evidence on the role of postprandial glycemia in the incidence of diabetic retinopathy (DR) in a real‐world setting. We aimed to assess the effect of postprandial hyperglycemia at clinic visits on the incidence of DR in patients with type 2 diabetes, and whether its effect differs depending on glycated hemoglobin (HbA1c) values and age. Materials and Methods: Intrapersonal mean blood glucose levels at 1–2 h post‐breakfast (1–2h‐PBBG), post‐lunch (1–2 h‐PLBG) and both (1–2h‐PBLBG) during 2 years from the first visit were used as baseline data. This retrospective cohort study enrolled 487, 323 and 406 patients who had 1–2h‐PBLBG, 1–2h‐PBBG and 1–2h‐PLBG measurements, respectively. These three groups were followed from 1999 up through 2017. Results: DR occurred in 145, 92 and 126 patients in the 1–2h‐PBLBG, 1–2h‐PBBG and 1–2h‐PLBG groups, respectively. Multivariate Cox regression analysis showed that the mean 1–2h‐PBLBG, 1–2h‐PBBG and 1–2h‐PLBG levels were significant predictors of DR, independent of mean HbA1c. In patients with mean HbA1c <7.0% and those with a baseline age <60 years, the mean 1–2h‐PBLBG, 1–2h‐PBBG and 1–2h‐PLBG levels were significant predictors. Conclusions: Postprandial hyperglycemia at clinic visits might predict the incidence of DR, independent of HbA1c. The effect of postprandial hyperglycemia on DR is obvious in patients with well‐controlled HbA1c and in younger patients. Even with the lower HbA1c level, correcting postprandial hyperglycemia is important for preventing DR, especially in middle‐aged adults with type 2 diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
8. Association Study of the Effect of WFS1 Polymorphisms on Risk of Type 2 Diabetes in Japanese Population
- Author
-
Mita,Masaki, Miyake,Kazuaki, Zenibayashi,Masako, Hirota,Yushi, Teranishi,Tetsuya, Kouyama,Kunichi, Sakaguchi,Kazuhiko, and Kasuga,Masato
- Subjects
Association study ,Type 2 diabetes ,WFS1 ,Single nucleotide polymorphism - Published
- 2008
9. Reduced Insulin Signaling and Endoplasmic Reticulum Stress Act Synergistically to Deteriorate Pancreatic β Cell Function
- Author
-
Matsuda, Tomokazu, Kido, Yoshiaki, Uchida, Tohru, and Kasuga, Masato
- Subjects
endoplasmic reticulum stress ,pancreatic β cell failure ,type 2 diabetes ,insulin signaling ,db/db mice - Published
- 2008
10. Establishment of maturity-onset diabetes of the young-induced pluripotent stem cells from a Japanese patient.
- Author
-
Yabe, Shigeharu G, Iwasaki, Naoko, Yasuda, Kazuki, Hamazaki, Tatsuo S, Konno, Masamitsu, Fukuda, Satsuki, Takeda, Fujie, Kasuga, Masato, and Okochi, Hitoshi
- Subjects
TYPE 2 diabetes ,PLURIPOTENT stem cells ,PANCREATIC cancer ,RNA ,JAPANESE people ,HEALTH - Abstract
Maturity-onset diabetes of the young ( MODY) is a heterozygous monogenic diabetes; more than 13 disease genes have been identified. However, the pathogenesis of MODY is not fully understood, because the pancreatic β-cells of the patients are inaccessable. Therefore, we attempted to establish MODY patient-derived induced pluripotent stem cells ( MODY- iPS) cells to investigate the pathogenic mechanism of MODY by inducing pancreatic β-cells. We established MODY5- iPS cells from a Japanese patient with MODY5 (R177X), and confirmed that MODY5- iPS cells possessed the characteristics of pluripotent stem cells. In the course of differentiation from MODY5- iPS cells into pancreatic β-cells, we examined the disease gene, HNF1B messenger ribonucleic acid. We found that the amount of R177X mutant transcripts was much less than that of wild ones, but they increased after adding cycloheximide to the medium. These results suggest that these R177X mutant messenger ribonucleic acids are disrupted by nonsense-mediated messenger ribonucleic acid decay in MODY- iPS cells during the developmental stages of pancreatic β-cells. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
11. Histidine Augments the Suppression of Hepatic Glucose Production by Central Insulin Action.
- Author
-
Kimura, Kumi, Nakamura, Yusuke, Inaba, Yuka, Matsumoto, Michihiro, Kido, Yoshiaki, Asahara, Shun-ichiro, Matsuda, Tomokazu, Watanabe, Hiroshi, Maeda, Akifumi, Inagaki, Fuyuhiko, Mukai, Chisato, Takeda, Kiyoshi, Akira, Shizuo, Ota, Tsuguhito, Nakabayashi, Hajime, Kaneko, Shuichi, Kasuga, Masato, and Inoue, Hiroshi
- Subjects
TYPE 2 diabetes ,GLUCOSE in the body ,HISTIDINE ,LIVER ,INSULIN - Abstract
Glucose intolerance in type 2 diabetes is related to enhanced hepatic glucose production (HGP) due to the increased expression of hepatic gluconeogenic enzymes. Previously, we revealed that hepatic STAT3 decreases the expression of hepatic gluconeogenic enzymes and suppresses HGP. Here, we show that increased plasma histidine results in hepatic STAT3 activation. Intravenous and intracerebroventricular (ICV) administration of histidine-activated hepatic STAT3 reduced G6Pase protein and mRNA levels and augmented HGP suppression by insulin. This suppression of hepatic gluconeogenesis by histidine was abolished by hepatic STAT3 deficiency or hepatic Kupffer cell depletion. Inhibition of HGP by histidine was also blocked by ICV administration of a his-tamine H1 receptor antagonist. Therefore, histidine activates hepatic STAT3 and suppresses HGP via central histamine action. Hepatic STAT3 phosphorylation after histidine ICV administration was attenuated in histamine H
1 receptor knockout (Hrh1KO) mice but not in neuron-specific insulin receptor knockout (NIRKO) mice. Conversely, hepatic STAT3 phosphorylation after insulin ICV administration was attenuated in NIRKO but not in Hrh1KO mice. These findings suggest that central histidine action is independent of central insulin action, while both have additive effects on HGP suppression. Our results indicate that central histidine/histamine-mediated suppression of HGP is a potential target for the treatment of type 2 diabetes. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
12. Role of KLF15 in Regulation of Hepatic Gluconeogenesis and Metformin Action.
- Author
-
Takashima, Mototsugu, Ogawa, Wataru, Hayashi, Kumiko, Inoue, Hiroshi, Kinoshita, Shinichi, Okamoto, Yasuo, Sakaue, Hiroshi, Wataoka, Yu, Emi, Aki, Senga, Yoko, Matsuki, Yasushi, Watanabe, Eijiro, Hiramatsu, Ryuji, and Kasuga, Masato
- Subjects
GLUCONEOGENESIS ,METFORMIN ,TYPE 2 diabetes ,HYPOGLYCEMIC agents ,AMINO acids - Abstract
OBJECTIVE--An increase in the rate of gluconeogenesis is largely responsible for the hyperglycemia in individuals with type 2 diabetes, with the antidiabetes action of metformin being thought to be achieved at least in part through suppression of gluconeogenesis. RESEARCH DESIGN AND METHODS--We investigated whether the transcription factor KLF15 has a role in the regulation of gluconeogenesis and whether KLF15 participates in the antidiabetes effect of metformin. RESULTS--Here we show that KLF15 regulates the expression of genes for gluconeogenic or amino acid-degrading enzymes in coordination with the transcriptional coactivator peroxisome proliferator-activated receptor γ coactivator 1α. Liver-specific ablation of KLF15 in diabetic mice resulted in downregulation of the expression of genes for gluconeogenic or amino acid catabolic enzymes and in amelioration of hyperglycemia. Exposure of cultured hepatocytes to metformin reduced the abundance of KLF15 through acceleration of its degradation and downregulation of its mRNA. Metformin suppressed the expression of genes for gluconeogenic or amino acid-degrading enzymes in cultured hepatocytes, and these effects of metformin were attenuated by restoration of KLF15 expression. Administration of metformin to mice inhibited both the expression of KLF15 and glucose production in the liver, the latter effect also being attenuated by restoration of hepatic KLF15 expression. CONCLUSIONS--KLF15 plays an important role in regulation of the expression of genes for gluconeogenic and amino acid-degrading enzymes and that the inhibitory effect of metformin on gluconeogenesis is mediated at least in part by downregulation of KLF15 and consequent attenuation of the expression of such genes. Diabetes 59:1608-1615, 2010 [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
13. Frequency of the G/G Genotype of Resistin Single Nucleotide Polymorphism at -420 Appears to Be Increased in Younger-Onset Type 2 Diabetes.
- Author
-
Ochi, Masaaki, Osawa, Haruhiko, Hirota, Yushi, Hara, Kazuo, Tabara, Yasuharu, Tokuyama, Yoshiharu, Shimizu, Ikki, Kanatsuka, Azuma, Fujii, Yasuhisa, Ohashi, Jun, Miki, Tetsuro, Nakamura, Naoto, Kadowaki, Takashi, Itakura, Mitsuo, Kasuga, Masato, and Makino, Hideichi
- Subjects
GENETIC polymorphisms ,NUCLEOTIDES ,CYTOKINES ,TYPE 2 diabetes ,DIABETES - Abstract
OBJECTIVE--Resistin is an adipocyte-secreted cytokine associated with insulin resistance in mice. We previously reported that the G/G genotype of a resistin single nucleotide polymorphism (SNP) at -420 increases type 2 diabetes susceptibility by enhancing its promoter activity. The aim of the present study was to determine the relevance of SNP -120 in a large number of subjects. RESEARCH DESIGN AND METHODS--We examined 2,610 type 2 diabetic case and 2,502 control subjects. The relation between SNP -420 and the age of type 2 diabetes onset was further analyzed by adding 237 type 2 diabetic subjects with age of onset ≤40 years. RESULTS--When analyzed without considering subject age, the SNP -420 genotype was not associated with type 2 diabetes. Since we reported that the onset of type 2 diabetes was earlier in G/G genotype, we analyzed the data using a trend test for age intervals of 10 years. The frequency of G/G genotype differed among age grades in type 2 diabetes (P = 0.037) and appeared to be higher in younger grades. In type 2 diabetes, G/G genotype was more frequent in subjects aged <40 years than in those aged ≥40 years (G/G vs. C/C, P = 0.003). In a total of 2,430 type 2 diabetic subjects with age of onset <60 years, the trend test showed that the G/G genotype had an increasing linear trend as the age grade of type 2 diabetes onset became younger (P = 0.0379). In control subjects, the frequency of C/G genotype showed an increasing linear trend with increasing age (P = 0.010). CONCLUSIONS--The G/G genotype frequency of resistin SNP -420 appears to be increased in younger-onset type 2 diabetic subjects. Diabetes 56:2834-2838, 2007 [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
14. Association Studies of Variants in the Genes Involved in Pancreatic β-Cell Function in Type 2 Diabetes in Japanese Subjects.
- Author
-
Yokoi, Norihide, Kanamori, Masao, Horikawa, Yukio, Takeda, Jun, Sanke, Tokio, Furuta, Hiroto, Nanjo, Kishio, Mori, Hiroyuki, Kasuga, Masato, Hara, Kazuo, Kadowaki, Takashi, Tanizawa, Yukio, Oka, Yoshitomo, Iwami, Yukiko, Ohgawara, Hisako, Yamada, Yuichiro, Seino, Yutaka, Yano, Hideki, Cox, Nancy J., and Seino, Susumu
- Subjects
INSULIN ,TYPE 2 diabetes ,GENES ,CELL physiology ,JAPANESE people ,DISEASES - Abstract
Because impaired insulin secretion is characteristic of type 2 diabetes in Asians, including Japanese, the genes involved in pancreatic β-cell function are candidate susceptibility genes for type 2 diabetes. We examined the association of variants in genes encoding several transcription factors (TCF1, TCF2, HNF4A, ISL1, IPF1, NEUROG3, PAX6, NKX2-2, NKX6-1, and NEUROD1) and genes encoding the ATP-sensitive K
+ channel subunits Kir6.2 (KCNJ11) and SUR1 (ABCC8) with type 2 diabetes in a Japanese cohort of 2,834 subjects. The exon 16 -3c/t variant rs1799854 in ABCC8 showed a significant association (P = 0.0073), and variants in several genes showed nominally significant associations (P < 0.05) with type 2 diabetes. Although the E23K variant rs5219 in KCNJ11 showed no association with diabetes in Japanese (for the K allele, odds ratio [OR] 1.08 [95% CI 0.97-1.21], P = 0.15), 95% CI around the OR overlaps in meta-analysis of European populations, suggesting that our results are not inconsistent with the previous studies. This is the largest association study so far conducted on these genes in Japanese and provides valuable information for comparison with other ethnic groups. Diabetes 55:2379-2386, 2006 [ABSTRACT FROM AUTHOR]- Published
- 2006
- Full Text
- View/download PDF
15. The IHPK1 gene is disrupted at the 3p21.31 breakpoint of t(3;9) in a family with type 2 diabetes mellitus.
- Author
-
Kamimura, Junichi, Wakui, Keiko, Kadowaki, Hiroko, Watanabe, Yukio, Miyake, Kazuaki, Harada, Naoki, Sakamoto, Michiyo, Kinoshita, Akira, Yoshiura, Koh-ichiro, Ohta, Tohru, Kishino, Tatsuya, Ishikawa, Mutsuo, Kasuga, Masato, Fukushima, Yoshimitsu, Niikawa, Norio, and Matsumoto, Naomichi
- Subjects
TYPE 2 diabetes ,INSULIN ,PATHOGENIC microorganisms ,CHROMOSOMAL translocation ,INOSITOL phosphates ,GENETICS ,GENES - Abstract
Type 2 diabetes mellitus (T2DM) is a group of multifactorial disorders due to either defective insulin secretion or action. Despite the fact that numerous genetic researches of T2DM have been pursued, the pathogenic mechanisms remain obscure. We encountered a T2DM family associated with a balanced reciprocal translocation, t(3;9)(p21.31;q33.1). To isolate a candidate gene susceptible to T2DM, we constructed physical maps covering both the 3p and 9q breakpoints of the translocation in the family. Consequently, the inositol hexaphosphate kinase 1 gene (IHPK1) (OMIM *606991) was found to be disrupted at the 3p21.31 breakpoint. We then carried out sequence analysis for all coding regions of IHPK1 in 405 unrelated T2DM patients in order to validate whether aberrations of the gene are common in T2DM patients, but we failed to detect any pathogenic changes. The disruption of IHPK1 or another predisposing gene affected by position effect of the translocation may explain the T2DM phenotype at least in this family. Alternatively, the IHPK1 disruption in the family is a chance association. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
16. Thirty-Year Trends in the Prevalence and Severity of Diabetic Retinopathy at the First Visit in Patients with Untreated Type 2 Diabetes Mellitus.
- Author
-
Kubota, Tetsuya, Todoroki-Mori, Kikue, Iwamoto, Masahiko, Kobori, Toshiko, Kikuchi, Takako, Tahara, Tazu, Onishi, Yukiko, Araki, Michihiro, Kasuga, Masato, and Yoshida, Yoko
- Subjects
- *
TYPE 2 diabetes , *DIABETIC retinopathy , *BLINDNESS ,DEVELOPED countries - Abstract
PurposeMethodsResultsConclusionAlthough the rate of diabetic retinopathy (DR)-related blindness has decreased in developed countries in recent years, the reasons for this decrease have remained unclear. The prevalence/severity trends of DR at the first visit in patients with untreated type 2 diabetes mellitus (T2DM) patients seen between the1986s and 2018s were assessed.A total of 1979 Japanese T2DM patients diagnosed between 1986 and 2018 were divided into four groups by the decade of their first visit: the 1986 years (1986–1987), the 1996 years (1996–1997), the 2006 years (2006–2008), the 2016 years (2016–2018). The DR prevalence/severity trends were assessed.A significant decrease in the rate of prevalence of DR from the 1986s to 2016s was observed among previously untreated T2DM patients visiting our hospital for the first time (1986s: 25.5%; 1996s: 26.2%; 2006s: 22.2%; and 2016s: 15.6%). The prevalence was significantly higher in females (30.2%) than in males (21.3%). Although the severity trend of DR did not differ significantly among the four measurement years, the rate of simple DR was the highest in the 2016s.We found, for the first time, a significant decrease in the rate of prevalence of DR from the 1986s to 2016s in patients with untreated T2DM visiting our hospital for the first time. A decrease in the rate of DR prevalence could explain, at least in part, the observed reduction in the rate of blindness in patients with T2DM. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Fat Stress and Liver Resistance.
- Author
-
Ogawa, Wotaru and Kasuga, Masato
- Subjects
- *
CELL communication , *LIVER cells , *FAT cells , *TYPE 2 diabetes , *IMMUNOLOGY of inflammation , *MACROPHAGE activation , *PROTEIN kinase C , *CYTOKINES , *INTERLEUKIN-6 , *INSULIN resistance , *INSULIN - Abstract
The article discusses communication between immune and fat cells in adipose tissue and hepatocytes and the underlying pathogenesis of obesity-related diabetes. Type 2 diabetes afflicts about 220 million worldwide and is characterized by a defect in the secretion of the hormone insulin and insulin resistance, which is a decrease in sensitivity to the hormone. Topics include an overview of research by Sabio and colleagues showing an inflammatory response within fat cells also plays a role in regulating insulin sensitivity in hepatocytes. Also discussed is the relationship between the production of proinflammatory cytokines such as interleukin (IL)-6 by macrophages and the protein kinase c-Jun NH2-terminal kinase 1 (JNK1).
- Published
- 2008
- Full Text
- View/download PDF
18. Erythromycin administration before sleep is effective in decreasing fasting hyperglycemia in type 2 diabetic patients.
- Author
-
Ueno, Naohiko, Inui, Akio, Asakawa, Akihiro, Takao, Fumiaka, Ichibangase, Akira, Komatsu, Yoshio, Kasuga, Masato, Ueno, N, Inui, A, Asakawa, A, Takao, F, Ichibangase, A, Komatsu, Y, and Kasuga, M
- Subjects
ERYTHROMYCIN ,INSULIN ,CARBOHYDRATES in the body ,TYPE 2 diabetes ,METABOLISM - Abstract
Presents a study which investigated the effect of erythromycin on insulin release and glycemic control in type 2 diabetic patients. Results; Other physiological effects of erythromycin.
- Published
- 2001
- Full Text
- View/download PDF
19. Analysis of the duration and extent of the legacy effect in patients with type 2 diabetes: A real-world longitudinal study.
- Author
-
Takao, Toshiko, Matsuyama, Yutaka, Suka, Machi, Yanagisawa, Hiroyuki, and Kasuga, Masato
- Abstract
Aims: To analyze the duration and extent of the legacy effect on diabetic complications in real-world patients with type 2 diabetes.Methods: This was a retrospective cohort study. We included the following three cohorts of patients: diabetic retinopathy (DR) (n = 1107), diabetic kidney disease (DKD) (n = 1486), and cardiovascular disease (CVD) (n = 1485). Patients were enrolled from 1995 to 1999 and followed up to 2017. Endpoints were DR incidence, ≥40% decrease in estimated glomerular filtration rate, and CVD incidence. The relationships between HbA1c as a time-dependent variable and the risk of reaching each endpoint were analyzed using multivariate Cox regression models.Results: A total of 313 patients developed DR, 316 developed DKD, and 177 developed CVD. Hazard ratios as a function of time-dependent HbA1c (moving mean) accumulated over time. This accumulation was largest for DR, followed by DKD and CVD. The hazard ratios for each endpoint reached a plateau during the preceding 14-19 years.Conclusions: The effect of past glycemic control may continue during 14-19 years, with a greater effect during ≤10 years. Therefore, the end of the legacy effect could be 15-20 years. This effect may be the greatest for DR, followed by DKD, and the smallest for CVD. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
20. Differential proteome analysis of serum proteins associated with the development of type 2 diabetes mellitus in the KK-Ay mouse model using the iTRAQ technique.
- Author
-
Takahashi, Eri, Okumura, Akinori, Unoki-Kubota, Hiroyuki, Hirano, Hisashi, Kasuga, Masato, and Kaburagi, Yasushi
- Subjects
- *
PROTEOMICS , *BLOOD proteins , *TYPE 2 diabetes , *LABORATORY mice , *GENE expression , *OBESITY complications - Abstract
Abstract: To identify candidate serum molecules associated with the progression of type 2 diabetes mellitus (T2DM), we carried out differential proteomic analysis using the KK-Ay mouse, an animal model of T2DM with obesity. We employed an iTRAQ-based quantitative proteomic approach to analyze the proteomic changes in the sera collected from a pair of 4-week-old KK-Ay versus C57BL/6 mice. Among the 227 proteins identified, a total of 45 proteins were differentially expressed in KK-Ay versus C57BL/6 mice. We comparatively analyzed a series of the sera collected at 4 and 12weeks of age from KK-Ay and C57BL/6 mice for the target protein using multiple reaction monitoring analysis, and identified 8 differentially expressed proteins between the sera of these mice at both time points. Among them, serine (or cysteine) peptidase inhibitor, clade A, member 3K (SERPINA3K) levels were elevated significantly in the sera of KK-Ay mice compared to C57BL/6 mice. An in vitro assay revealed that the human homologue SERPINA3 increased the transendothelial permeability of retinal microvascular endothelial cells, which may be involved in the pathogenesis of diabetes and/or diabetic retinopathy. With the identified proteins, our proteomics study could provide valuable clues for a better understanding of the underlying mechanisms associated with T2DM. Biological significance: In this paper, we investigated the serum proteome of KK-Ay mice in a pre-diabetic state compared to that of wild type controls in an attempt to uncover early diagnostic markers of diabetes that are maintained through a diabetic phenotype. We used iTRAQ-based two-dimensional LC–MS/MS serum profiling, and identified several differentially expressed proteins at the pre-diabetic stage. The differential expression was confirmed by multiple reaction monitoring assay, which is fast gaining ground as a sensitive, specific, and cost-effective methodology for relative quantification of the candidate proteins. Using these techniques, we have identified eight candidate proteins of interest including SERPINA3K, which may be important in the pathology of T2DM and/or diabetic retinopathy. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
21. Increased ribosomal biogenesis induces pancreatic β cell failure in mice model of type 2 diabetes
- Author
-
Asahara, Shun-ichiro, Matsuda, Tomokazu, Kido, Yoshiaki, and Kasuga, Masato
- Subjects
- *
GENETICS of type 2 diabetes , *PANCREATIC beta cells , *GENE expression , *HYPERPLASIA , *ANIMAL models in research , *INSULIN resistance , *LABORATORY mice - Abstract
Abstract: Aim: To study the changes in gene expression by pancreatic β cells under insulin resistance conditions. Method: An exhaustive gene expression analysis was performed, using isolated pancreatic islets of obese diabetic model Lepr−/− mice. Overexpression of cyclin D2 was induced in cells from the pancreatic β cell line, namely, INS-1. Results: Through a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, we found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found. Conclusion: In cases of pancreatic β cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
22. PKCλ regulates glucose-induced insulin secretion through modulation of gene expression in pancreatic Β cells.
- Author
-
Hashimoto, Naoko, Kido, Yoshiaki, Uchida, Tohru, Matsuda, Tomokazu, Suzuki, Kazuhisa, Inoue, Hiroshi, Matsumoto, Michihiro, Ogawa, Wataru, Maeda, Sakan, Fujihara, Hiroaki, Ueta, Yoichi, Uchiyama, Yasuo, Akimoto, Kazunori, Ohno, Shigeo, Noda, Tetsuo, and Kasuga, Masato
- Subjects
- *
TYPE 2 diabetes , *INSULIN , *GLUCOSE , *GLUCOKINASE , *GENES , *DIABETES - Abstract
Altered regulation of insulin secretion by glucose is characteristic of individuals with type 2 diabetes mellitus, although the mechanisms that underlie this change remain unclear. We have now generated mice that lack the &lamda; isoform of PKC in pancreatic β cells (βPKC&lamda;-/- mice) and show that these animals manifest impaired glucose tolerance and hypoinsulinemia. Furthermore, insulin secretion in response to high concentrations of glucose was impaired, whereas the basal rate of insulin release was increased, in islets isolated from βPKC&lamda;-/- mice. Neither the β cell mass nor the islet insulin content of βPKC&lamda;-/- mice differed from that of control mice, however. The abundance of mRNAs for Glut2 and HNF3β was reduced in islets of βPKC&lamda;-/- mice, and the expression of genes regulated by HNF3β was also affected (that of Sur1 and Kir6.2 genes was reduced, whereas that of hexokinase 1 and hexokinase 2 genes was increased). Normalization of HNF3β expression by infection of islets from βPKC&lamda;-/- mice with an adenoviral vector significantly reversed the defect in glucose-stimulated insulin secretion. These results indicate that PKC&lamda; plays a prominent role in regulation of glucose-induced insulin secretion by modulating the expression of genes important for β cell function. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
23. Diabetic retinopathy in non-insulin-dependent diabetes mellitus patients: the role of gliclazide.
- Author
-
Akanuma, Yasuo, Kosaka, Kinori, Kanazawa, Yasunori, Kasuga, Masato, Fukuda, Masatoshi, Aoki, Shigenobu, Akanuma, Y, Kosaka, K, Kanazawa, Y, Kasuga, M, Fukuda, M, and Aoki, S
- Subjects
- *
DIABETIC retinopathy , *DIABETES complications , *GLICLAZIDE , *HYPOGLYCEMIC sulfonylureas , *TYPE 2 diabetes complications , *CLINICAL trials , *COMPARATIVE studies , *RESEARCH methodology , *MEDICAL cooperation , *TYPE 2 diabetes , *RESEARCH , *EVALUATION research , *RANDOMIZED controlled trials , *THERAPEUTICS - Abstract
Diabetic retinopathy is the most common cause of human blindness between the ages of 30 and 67 in the industrialized world. Retinopathy has a multifactorial etiology. Standard treatment has aimed at correcting only disturbed glucose metabolism, but this may lead only to the partial amelioration of certain hemobiologic factors. In addition to its metabolic action, gliclazide has been shown to have specific hemobiologic properties; studies with gliclazide in animals and humans have shown significant improvements of platelet abnormalities, stimulation of prostaglandin I2 synthesis, and enhancement of fibrinolytic activity. In humans, open-label studies have shown that gliclazide treatment leads to stabilization of background retinopathy in non-insulin-dependent diabetics and, more recently, these beneficial effects have been confirmed in controlled studies lasting up to 37 months in which other sulfonylureas were used. The Japanese Diabetic Retinopathy Program studied the progression of retinopathy over a 5-year period, comparing gliclazide with other sulfonylureas and with placebo. This study showed that, with equivalent metabolic control, there was a trend toward a lower rate of deterioration and a significantly lower incidence of preproliferative retinopathy in the group receiving gliclazide compared with those receiving other sulfonylureas. Overall, the specific hemobiologic actions of gliclazide appear to offset or retard the progression of diabetic retinopathy and may have the advantage of lowering the incidence of preproliferative retinopathy. [ABSTRACT FROM AUTHOR]
- Published
- 1991
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.