1. Loss of the ubiquitin conjugating enzyme UBE2E3 induces cellular senescence.
- Author
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Plafker KS, Zyla K, Berry W, and Plafker SM
- Subjects
- Cyclin-Dependent Kinase Inhibitor p16 genetics, Cyclin-Dependent Kinase Inhibitor p21 genetics, DNA Damage genetics, Homeostasis genetics, Humans, Mitochondria genetics, Tumor Suppressor Protein p53 genetics, Ubiquitin genetics, Autophagy genetics, Cellular Senescence genetics, Ubiquitin-Conjugating Enzymes genetics
- Abstract
Cellular senescence plays essential roles in tissue homeostasis as well as a host of diseases ranging from cancers to age-related neurodegeneration. Various molecular pathways can induce senescence and these different pathways dictate the phenotypic and metabolic changes that accompany the transition to, and maintenance of, the senescence state. Here, we describe a novel senescence phenotype induced by depletion of UBE2E3, a highly-conserved, metazoan ubiquitin conjugating enzyme. Cells depleted of UBE2E3 become senescent in the absence of overt DNA damage and have a distinct senescence-associated secretory phenotype, increased mitochondrial and lysosomal mass, an increased sensitivity to mitochondrial and lysosomal poisons, and an increased basal autophagic flux. This senescence phenotype can be partially suppressed by co-depletion of either p53 or its cognate target gene, p21
CIP1/WAF1 , or by co-depleting the tumor suppressor p16INK4a . Together, these data describe a direct link of a ubiquitin conjugating enzyme to cellular senescence and further underscore the consequences of disrupting the integration between the ubiquitin proteolysis system and the autophagy machinery., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2018
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