1. In vitro multi-modal imaging of magnetic targeted nanoparticles and their targeting effect on hepatic stellate cells.
- Author
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Li Xuan, Lu Min, Li Mingxing, Ao Meng, Tang Linmei, Zeng Zhen, Hu Jingwei, Huang Zhiqiang, and Xuan Jiqing
- Subjects
LIVER cells ,NANOTECHNOLOGY ,MAGNETIC nanoparticles ,MOLECULAR probes ,MAGNETIC particles ,CONTRAST-enhanced ultrasound ,ULTRASONIC imaging - Abstract
BACKGROUND: In recent years, molecular imaging combined with medical imaging technology and targeted molecular probes have gradually become a research focus. The targeted tissues at the molecular level can be observed using molecular imaging, medical imaging technology, and targeted molecular probes in combination to realize non-invasive imaging of the occurrence and development of the diseases. OBJECTIVE: To develop the magnetic targeted nanoparticle probes, observe the ultrasound/CT/MRI imaging properties in vitro, and investigate their targeting ability to rat hepatic stellate cells in vitro. METHODS: Taking poly(lactic-co-glycolic acid) (PLGA) polymer as the shell, cyclic arginine-glycine-aspartic acid (eRGO) octapeptide as the ligand, targeted magnetic nanoparticles with superparamagnetic Fe
3 O4 embedded in the shell and perfluorooctyl bromide(PFOB) loaded in the core were prepared by double emulsion evaporation method. The physical and chemical properties of the nanoparticles were detected. The ultrasound/CT/MRI multi-modal imaging properties of the nanoparticles at different concentrations diluted with double-distilled water were tested in vitro. Cyclic RGD peptide immobilization on PLGA-Fe3 O4 -PFOB NPs was completed through the amide condensation reaction. The conjugation efficiency of the eRGO on PLGA-Fe3 O4 -PFOB NPs and targeting ability of targeted magnetic nanoparticles in vitro were verified. Cytotoxicity experiments were used to measure the toxic effects of nanoparticles at different concentrations on BRL-3A cells in each group. RESULTS AND CONCLUSION: The targeted magnetic nanoparticles with the average size of (221.5±60.3) nm were uniform in dispersion and size. The prepared individual nanoparticle was spherical with the superparamagnetic Fe3 O4 scattered on the shell. The encapsulation rate of Fe3 O4 was 38%. In vitro ultrasound imaging and CT imaging signal decreased gradually as the concentrations of the nanoparticle suspension decreased. The T2-weighted signal of MRI decreased gradually with the increase of the concentrations of magnetic particle Fe3 O4 . Flow cytometry results showed that 94.13% of the eRGD was bound to the nanoparticles. In vitro cell targeting experiments showed that compared to PLGA-Fe3 O4 -PFOB NPs, cRGD-PLGA-Fe3 O4 -PFOB NPs exhibited greater cell targeting and affinity efficiency to hepatic stellate cells. Cytotoxicity experiments results showed the nanoparticles had no significant influence on cell viability of the BRL-3A cells. These results suggest that targeted magnetic nanoprobe cannot only be used as a multi-modal imaging contrast agent for ultrasound/CT/MRI, but also exhibits a strong specific affinity to rat hepatic stellate cells in vitro. It has great potential for the early diagnosis of liver fibrosis. [ABSTRACT FROM AUTHOR]- Published
- 2020
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