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Fifty million people worldwide are affected by dementia, a heterogeneous neurodegenerative condition encompassing diseases such as Alzheimer's, vascular dementia, and Parkinson's. For them, cognitive decline is often the first marker of the pathology after irreversible brain damage has already occurred. Researchers now believe that structural and functional alterations of the brain vasculature could be early precursors of the diseases and are looking at how functional imaging could provide an early diagnosis years before irreversible clinical symptoms. In this preclinical pilot study, we proposed using functional ultrasound (fUS) on the retina to assess neurovascular alterations non-invasively, bypassing the skull limitation. We demonstrated for the first time the use of functional ultrasound in the retina and applied it to characterize the retinal hemodynamic response function in vivo in rats following a visual stimulus. We then demonstrated that retinal fUS could measure robust neurovascular coupling alterations between wild-type rats and TgF344-AD rat models of Alzheimer's disease. We observed an average relative increase in blood volume of 21% in the WT versus 37% for the TG group (p = 0.019). As a portable, non-invasive and inexpensive technique, rfUS is a promising functional screening tool in clinics for dementia years before symptoms. [ABSTRACT FROM AUTHOR]

Purpose: Sensitivity of contrast-enhanced ultrasound (CEUS) to microvascular flow modifications can be limited by intra-injection variability (injected dose, rate, volume).Procedures: To evaluate the effect of injection variability on microvascular flow evaluation, CEUS was compared between controlled and manual injections where enhancement was assessed in vitro within a flow phantom, in normal murine kidney (N = 12) and in murine ectopic tumors (N = 10).Results: For both in vitro and in vivo measurements in the renal cortex, controlled injections significantly improved reproducibility of functional parameter estimation. Their coefficient of variation (CV) in the renal cortex ranged from 4 to 19 % for controlled injection vs. 5 to 43 % for manual injections. For measurements in tumors, controlled injection only decreased the CV significantly for the mean transit time. In tumors, multiple injections of contrast agent with a 15-min delay between each were shown to strongly modify contrast uptake by facilitating penetration of microbubbles.Conclusion: Improved reproducibility of CEUS assessments in murine models should provide more robust quantification of flow parameters and more sensitive evaluation of tumor modifications in therapeutic models. [ABSTRACT FROM AUTHOR]

Shear wave elastography helps physicians to characterize pathologies by assessing biomechanical properties of soft tissues. Compared with classical rheology, these techniques allow the quantification of the mechanical properties of tissues in the frequency range of hundreds of hertz. In this paper, ultrasound elastographic measurements and classical rheology are compared over a frequency range spanning five orders of magnitude [0.01 to 1200 Hz] to characterize model gels at multiple scales. Hybrid hydrogels were specially synthesized to get a fine tuning of the material dissipative response. Straincontrolled rheology (SCR) experiments were performed to get the elastic moduli G" and loss moduli G" from 0.01 Hz to 10 Hz and were confirmed by tensile tests. Transient elastography (TE from 50 to 400 Hz) and supersonic shear imaging (SSI from 200 to 1200 Hz) were used to characterize polymers at high frequency. Two different hydrogels were tested in the ultrasound setup with different concentration of scatterers. From low-frequency measurements, elastic moduli were extrapolated at high frequency and a very good correlation was obtained between SCR and TE and between SCR and SSI (r = 0.92 and r = 0.95, respectively). This paper demonstrates the capability of shear wave elastography to accurately image rheological properties of soft tissues, to differentiate soft elastic domains from viscous ones. It also gives new insights into soft material science because it provides a rheological tool in a high-frequency domain complementary to conventional rheometry. [ABSTRACT FROM AUTHOR]

Despite recent improvements in medical imaging, the final diagnosis and biopathologic characterization of breast cancers currently still requires biopsies. Ultrasound is commonly used for clinical examination of breast masses. B-Mode and shear wave elastography (SWE) are already widely used to detect suspicious masses and differentiate benign lesions from cancers. But additional ultrasound modalities such as backscatter tensor imaging (BTI) could provide relevant biomarkers related to tissue organization. Here we describe a 3-D multiparametric ultrasound approach applied to breast carcinomas in the aims of (i) validating the ability of BTI to reveal the underlying organization of collagen fibers and (ii) assessing the complementarity of SWE and BTI to reveal biopathologic features of diagnostic interest. Three-dimensional SWE and BTI were performed ex vivo on 64 human breast carcinoma samples using a linear ultrasound probe moved by a set of motors. Here we describe a 3-D multiparametric representation of the breast masses and quantitative measurements combining B-mode, SWE and BTI. Our results reveal for the first time that BTI can capture the orientation of the collagen fibers around tumors. BTI was found to be a relevant marker for assessing cancer stages, revealing a more tangent tissue orientation for in situ carcinomas than for invasive cancers. In invasive cases, the combination of BTI and SWE parameters allowed for classification of invasive tumors with respect to their grade with an accuracy of 95.7%. Our results highlight the potential of 3-D multiparametric ultrasound imaging for biopathologic characterization of breast tumors. [ABSTRACT FROM AUTHOR]