1. Mitochondrial and mitochondrial-related nuclear genetic function in rabbit urinary bladder following reversal of outlet obstruction.
- Author
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Nevel-McGarvey CA, Rohrmann D, Levin RM, and Hudson AP
- Subjects
- Animals, Cell Nucleus metabolism, Citrate (si)-Synthase metabolism, Electron Transport Complex IV metabolism, Hypertrophy, Male, Mitochondria metabolism, Rabbits, Urinary Bladder metabolism, Urinary Bladder pathology, Urinary Bladder Neck Obstruction genetics, DNA, Mitochondrial metabolism, Mitochondria physiology, Urinary Bladder physiopathology, Urinary Bladder Neck Obstruction physiopathology
- Abstract
Partial outlet obstruction of the rabbit urinary bladder causes increased tissue hypertrophy and decreased contractility of that organ; we showed that, in an experimental rabbit model, both correlate closely with alterations in the status and expression of mitochondrial (mt), and mt-related nuclear, genetic parameters in bladder smooth muscle. Here we investigate the rate and overall level of recovery of mt and nuclear genetic function following reversal of outlet obstruction in the same animal model. Release from outlet obstruction at 28 days resulted in improvement in both level of hypertrophy and contractile function in all bladders studied. However, bladders fell into two groups based on whether relative copy mt genome number per cell was above or below that of unobstructed controls. Bladders with high mt DNA content adjusted organellar genome copy number toward normal post-reversal but did not properly adjust mt transcript levels; mt-related nuclear transcripts in these samples showed recovery. Bladders with low mt DNA content showed no adjustment of those levels toward normal post-reversal but did show some adjustment in other mt and nuclear genetic parameters. Thus, a limiting factor for return of normal bladder function following reversal of outlet obstruction may be recovery of normal mt genetic performance.
- Published
- 1999
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