1. Evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant Peroxiredoxin (Prx) and challenged with Fasciola hepatica.
- Author
-
Mendes RE, Pérez-Ecija RA, Zafra R, Buffoni L, Martínez-Moreno A, Dalton JP, Mulcahy G, and Pérez J
- Subjects
- Adjuvants, Immunologic administration & dosage, Animals, Antibodies, Helminth blood, CD2 Antigens analysis, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Fascioliasis immunology, Fascioliasis pathology, Fascioliasis prevention & control, Goat Diseases immunology, Goat Diseases pathology, Goats, Immunoglobulin G blood, Immunohistochemistry, Interferon-gamma analysis, Interleukin-4 analysis, Liver parasitology, Quillaja Saponins, Receptors, Antigen, T-Cell analysis, Saponins administration & dosage, Vaccination methods, Fasciola hepatica immunology, Fascioliasis veterinary, Goat Diseases prevention & control, Liver pathology, Peroxiredoxins immunology, Vaccines immunology
- Abstract
Protection against Fasciola hepatica in goats immunized with Peroxiredoxin (Prx) was assessed. The experimental trial consisted of three groups of seven animals; group 1 were unimmunized and uninfected, group 2 were immunized with adjuvant only and group 3 were immunized with recombinant Prx in adjuvant (immunized and infected). Immunization with Prx in Quil A adjuvant, group 3, induced a reduction in fluke burden of 33.04% when compared to adjuvant control, group 2, although this difference was not significant. The hepatic gross and microscopical morphometric study revealed lower damage in the Prx-immunized compared to group 2 (p<0.05). Furthermore, immunohistochemical studies revealed that the Prx-immunized group exhibited reduced infiltration of CD4(+), CD8(+), IFN-gamma(+) and TCR(+) (p<0.05); and CD2(+) and IL-4(+) (p<0.001) in hepatic lesions. Levels of anti-Prx serum IgG in group 3 showed a significant increase at the 4th week after challenge infection compared with group 2 (p<0.0001). This is the first report of ruminant immunization with recombinant Prx of F. hepatica. The study shows that this vaccine significantly reduces hepatic damage and encourages further studies to improve the vaccine efficacy., ((c) 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF