1. New vaccinia virus promoter as a potential candidate for future vaccines.
- Author
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Di Pilato M, Mejías-Pérez E, Gómez CE, Perdiguero B, Sorzano COS, and Esteban M
- Subjects
- Animals, Base Sequence, CD8-Positive T-Lymphocytes metabolism, Cytokines metabolism, Green Fluorescent Proteins genetics, Mice, Vaccinia prevention & control, Vaccinia virus immunology, Vaccinia virus physiology, CD8-Positive T-Lymphocytes immunology, Drug Design, Green Fluorescent Proteins immunology, Promoter Regions, Genetic genetics, Vaccinia immunology, Vaccinia virus genetics, Viral Vaccines genetics
- Abstract
Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines.
- Published
- 2013
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