1. Expression of aquaporins, vasopressin type 2 receptor, and Na+-K+-Cl- cotransporters in the rat endolymphatic sac.
- Author
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Nishimura, Masahiko, Kakigi, Akinobu, Takeda, Taizo, Takeda, Setsuko, and Doi, Katsumi
- Subjects
VASOPRESSIN ,MEMBRANE proteins ,GENE expression ,AQUAPORINS ,LABORATORY rats ,HOMEOSTASIS - Abstract
Conclusion. Since many water channels and pumps, such as aquaporin (AQP) 1-4, AQP6-9, vasopressin type 2 receptor (V2-R), Na+-K+-Cl- cotransporter (NKCC) 1, and NKCC2 were expressed in the rat endolymphatic sac, it should play an important role in the physiological function of acid-based metabolism and water balance in endolymphatic fluid homeostasis. Objective: To evaluate the expression and immunolocalization of AQP1-9, V2-R, NKCC1, and NKCC2 in the rat endolymphatic sac. Materials and methods. Wistar rats were used. Expression of AQP1-9, V2-R, NKCC1, and NKCC2 mRNA in the rat endolymphatic sac was investigated using the reverse transcription-polymerase chain reaction (RT-PCR) method, and detailed immunolocalization of AQP1-9, V2-R, NKCC1, and NKCC2 was investigated using immunohistochemical methods, including immunofluorescence microscopy. Results. mRNAs and proteins of AQP1-4, AQP6-9, V2-R, NKCC1, and NKCC2 were expressed, but AQP5 was not expressed in the rat endolymphatic sac. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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