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Objective: To establish pc DNA3.1-snail eukaryotic recombinant expression plasmid. Methods: Human Snail gene c DNA was amplified by reverse transcription polymerase chain reaction (RT-PCR). After digested by BamH I, EcoR I and ligation, Snail was inserted into pc DNA3. 1(+) eukaryotic expression vector. The positive colonies were screened and identified by single,double enzyme digest,agarose gel electrophoresis and DNA sequence analysis. pc DNA3. 1-snail plasmid was then transfected into siha cell line with Turbo Fect Transfection Reagent. Western-blot was used to detect the protein expression of snail. Results: The pc DNA3.1-snail recombinant plasmid was verified by enzyme digestion electrophoresis showed the expected fragment. The inserted sequence in pc DNA3.1(+)-snail was the same as the sequence of snail c DNA published in NCBI Gen Bank. Western blot results validated the recombinant plasmid expressed in siha cell line efficiently. Conclusion: The eukaryotic recombinant expression plasmid of pc DNA3.1 (+)-snail was successfully constructed. [ABSTRACT FROM AUTHOR]