1. Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma.
- Author
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Deng Z, Hasegawa M, Kiyuna A, Matayoshi S, Uehara T, Agena S, Yamashita Y, Ogawa K, Maeda H, and Suzuki M
- Subjects
- DNA, Viral analysis, Female, Genes, Viral, Humans, Male, Middle Aged, Papillomaviridae genetics, Prospective Studies, Real-Time Polymerase Chain Reaction, Carcinoma, Squamous Cell virology, Head and Neck Neoplasms virology, Oncogene Proteins, Viral genetics, Papillomavirus E7 Proteins genetics, RNA, Messenger metabolism, Repressor Proteins genetics, Viral Load
- Abstract
Background: The purpose of this study was to determine prospectively both human papillomavirus (HPV) load and physical status in different types of head and neck squamous cell carcinoma (HNSCC)., Methods: HPV DNA, E6/E7 mRNA expression, viral load, and physical status of 184 patients with HNSCC were examined simultaneously by polymerase chain reaction (PCR)-based methods., Results: The HPV genome was detected in 54 HNSCC samples (29.3%), particularly in tonsillar carcinomas (69.6%). Compared with nonoropharyngeal HNSCC, oropharyngeal carcinoma harbored a relatively higher viral load, especially in tonsillar carcinoma. Although integrated or mixed status was observed in 75.6% of HPV-16-positive samples, E6/E7 mRNA transcripts were detected in only 27.5% of HPV DNA-positive cases. High HPV-16 load correlated significantly with E6/E7 mRNA expression., Conclusion: E6/E7 mRNA expression in patients with HNSCC with low viral load remains low even in cases of integration to the host genome. Tonsillar carcinomas were significantly associated with HPV among various types of HNSCC., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2013
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