1. Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
- Author
-
Justin J. Jeffery, Timothy P. Sheahan, Aleksey Yevtodiyenko, Andrew J. Peat, Donald Creech, Kevin P. Francis, Andrew Mehle, Vy Tran, Daniel S. Poole, and Shihyun You
- Subjects
viruses ,lcsh:QR1-502 ,Biology ,medicine.disease_cause ,Antiviral Agents ,Multimodal Imaging ,lcsh:Microbiology ,Virus ,influenza virus ,reporter virus ,Viral life cycle ,Orthomyxoviridae Infections ,In vivo ,Genes, Reporter ,Virology ,Influenza A virus ,medicine ,multiplicity reactivation ,Bioluminescence ,Animals ,Whole Body Imaging ,multi-modal imaging ,Mice, Inbred BALB C ,Staining and Labeling ,Communication ,Optical Imaging ,Influenza a ,Viral Load ,In vitro ,3. Good health ,Disease Models, Animal ,Infectious Diseases ,Treatment Outcome ,J0101 ,Luminescent Measurements ,Female ,Viral load - Abstract
Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
- Published
- 2015