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Nanoparticle-assisted polymerase chain reaction (nanoPCR) is a novel method for the simple, rapid, and specific amplification of DNA and has been used to detect viruses. A duplex nanoPCR molecular detection system was developed to detect pseudorabies virus (PRV) and porcine bocavirus (PBoV). Primers were selected to target conserved regions within the PRV gE gene and the PBoV NS1 gene. Under optimized nanoPCR reaction conditions, two specific fragments of 316 bp (PRV) and 996 bp (PBoV) were amplified by the duplex nanoPCR with a detection limit of 6 copies for PRV and 95 copies for PBoV; no fragments were amplified when other porcine viruses were used as template. When used to test 550 clinical samples, the duplex nanoPRC assay and a conventional duplex PCR assay provided very similar results (98.1% consistency); single PRV infections, single PBoV infections, and concurrent PRV and PBoV infections were detected in 37%, 15%, and 9% of the samples, respectively. The results indicate that the novel duplex nanoPCR assay is useful for the rapid detection of PRV and PBoV in pigs. [ABSTRACT FROM AUTHOR]

Highlights: [•] NanoPCR is a novel method for amplifying DNA; here a nanoPCR assay was developed to detect PBoV. [•] The nanoPCR assay was 100-fold more sensitive than a conventional PCR assay. [•] PBoV positive rate was 71.3% by PBoV nanoPCR which demonstrated PBoV is epidemic in China. [Copyright &y& Elsevier]

Highlights: [•] Porcine teschovirus (PTV) is widespread and increasing in swine herds in northeast of China. [•] Four serotypes (PTV-2, -4, -6, and -8) were detected. [•] Recombination among PTV serotypes has occurred evidently in the field. [•] PTV commonly occurs with other viruses of swine. [•] The relationship between PTV and disease is unclear. [ABSTRACT FROM AUTHOR]

Abstract: Porcine parvovirus (PPV) is a major causative agent of reproductive failure in swine, which currently affects the swine industry worldwide. Although PPV was identified several years ago, little is known about how it overcomes host innate immunity. In this study, we used quantitative real-time PCR and a luciferase reporter assay to determine whether PPV infection induces type I interferon (IFN-α and IFN-β) and whether PPV infection blocks dsRNA-induced IFN-β promoter activation in cell cultures. The results indicate that PPV does not induce type I interferon and that the NS2 protein of PPV could blocks dsRNA-induced IFN-β promoter activation. [Copyright &y& Elsevier]