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Slightly acidic electrolytic water (SAEW) treatment for seed germination is a promising technique for sustainable agriculture. This study investigated the antioxidant activity of germinated sesame seeds treated with SAEW for the first time. Specifically, the impact and correlation of SAEW on the activities of total phenols, total flavonoids, and antioxidant oxidase in sesame seeds were examined. The results showed that SAEW with low ACC inhibited sesame germination, SAEW with high ACC promoted sesame germination, and sesame buds treated with SAEW with 30 mg/L and 50 mg/L ACC showed lower antioxidant activity and total phenolic and flavone content compared to tap water. In contrast, SAEW with 30 mg/L ACC had no significant effect on sesame growth but positively influenced the antioxidant activity of sesame seed germination by promoting phenolic compound synthesis through increased phenylalanine ammonia-lyase (PAL) activity and enhancing antioxidant activity by boosting PAL, polyphenol oxidase (PPO), and peroxidase (POD) activities. Generally, antioxidant ability was the most prominent in SAEW with 30 mg/L ACC, and positive correlations between antioxidation and total phenols and flavonoids content were found in sesame. These findings provide valuable insights into the mechanisms underlying the enhanced antioxidant capacity observed in germinated sesame seeds under SAEW stress. [ABSTRACT FROM AUTHOR]

Here, the bactericidal efficacy and mechanism of action of the combination of alkaline electrolyzed water (AlEW) and acidic electrolyzed water (AcEW) on Pseudomonas aeruginosa biofilm were investigated, in comparison with single AcEW or AlEW treatment. The mature P. aeruginosa biofilm was formed at 120 h of cultivation. The number of bacteria in the P. aeruginosa biofilm decreased by 7.87 and 2.51 Lg CFU/mL after single AcEW and single AlEW treatment for 8 min, while the sterilization rate reached 92.2% and 33.1%, respectively. The group of AcEW (7 min) co‐action with AlEW (1 min) had a P. aeruginosa sterilization rate of 85%. The AlEW (3 min) co‐action with AcEW (5 min) treatment showed the best bactericidal efficacy. With this treatment, the sterilization rate achieved 94.4%, and the extracellular protein, polysaccharide, and DNA were degraded with the ratios of 70.8%, 77.7%, and 70.5%, respectively. Thus, AlEW exhibited a major washing effect, which can destroy the biofilm structure of extracellular polymers to a certain extent, while AcEW displayed a significant bactericidal effect. These results suggest that the AlEW co‐action with AcEW treatment may be a promising candidate suitable for bacterial biofilm sterilization. [ABSTRACT FROM AUTHOR]

Previous studies have confirmed that electrolyzed water had disinfection potential and enrich functional nutrients during seed germination. However, the effect of slightly acidic electrolyzed water (SAEW) on the quality and safety of peanut sprouts is poorly understood. In this study, the influence and mechanism of SAEW on the antibacterial, antioxidant capacity, and allergenicity of peanut sprouts were investigated. Although SAEW‐3 with 33.85 mg/L available chlorine concentration (ACC) showed better antibacterial effect, the SAEW‐2 (23.74 mg/L ACC) group has a 20% and 50% increase in phenolic acid and γ‐aminobutyric acid content, respectively. Moreover, SAEW‐2 induced peanut sprout has the best antioxidant capacity by eliminating free radicals and improving peroxidase activity. SAEW‐2 or SAEW‐3 treatment contributed to decreasing Ara h 1 and Ara h 2 content thus reduce allergenicity. Therefore, SAEW with appropriate ACC could be a promising application in food safety, nutrients enrichment, and health‐improvement of peanut products. Novelty impact statement: Slightly acidic electrolyzed water (SAEW) with 23.74 mg/L available chlorine concentration (ACC) has a significant positive effect on the enrichment of phenolic and γ‐aminobutyric acid in germinated peanut.With the help of SAEW, the germination process can further reduce the content of Ara h 1 and Ara h 2, thereby reducing allergenicity.SAEW with appropriate ACC could be a promising application in food safety, nutrients enrichment, and health‐improvement of peanut products. [ABSTRACT FROM AUTHOR]

In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on L. monocytogenes were evaluated. The results showed that the strains of L. monocytogenes were killed completely within 30 s by SAEW whose available chlorine concentration (ACC) was higher than 12 mg/L, and it was confirmed that ACC is the main factor affecting the disinfection efficacy of SAEW. Moreover, our results demonstrated that SAEW could destroy the cell membrane of L. monocytogenes, which was observed by SEM and FT-IR, thus resulting in the leakage of intracellular substances including electrolyte, protein and nucleic acid, and DNA damage. On the other hand, the results found that SAEW could disrupt the intracellular ROS balance of L. monocytogenes by inhibiting the antioxidant enzyme activity, thus promoting the death of L. monocytogenes. In conclusion, the bactericidal mechanism of SAEW on L. monocytogenes was explained from two aspects including the damage of the cell membrane and the breaking of ROS balance. [ABSTRACT FROM AUTHOR]

[Display omitted] • DEPs related to energy metabolism and oxidative stress were found contributing to SAEW treated buckwheat sprouts growth. • Bioinformatics analysis revealed 118 GO categories were in relation to molecular function. • A total of 9 DEPs (5 up-regulated) were mapped to 10 significantly enriched KEGG pathways in SAEW group. • SAEW increased flavonoids accumulation by modulating zymoproteins in phenylpropanoid biosynthesis pathway. Previous work has demonstrated that slightly acidic electrolyzed water (SAEW) can promote growth and nutrient enrichment of buckwheat sprouts. In this study, iTRAQ-based quantitative proteomic analysis of SAEW-induced buckwheat sprouts was conducted to explore its mechanism of action. The results showed that 11, 10 and 14 differentially expressed proteins (DEPs) related to energy metabolism, oxidative stress and flavonoid biosynthesis accumulated upwards and downwards, respectively, in SAEW-treated buckwheat. Bioinformatics analysis revealed 118 GO categories were in relation to molecular function. In the SAEW group, a total of 9 DEPs (5 up-regulated) were mapped to 10 significantly enriched KEGG pathways. SAEW induced flavonoid enrichment by modulating zymoproteins (e.g. phenylalanine ammonialyase and flavonol synthase) in phenylpropanoid biosynthesis pathway. qRT-PCR results had consistency with abundance levels of their corresponding proteins. These findings are likely to reveal the molecular mechanisms underlying the biochemical enrichment of buckwheat sprouts by SAEW. [ABSTRACT FROM AUTHOR]