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We revisit the localization computation of the expectation values of 't Hooft operators in N = 2* SU(N) theory on ℝ³ x S¹. We show that the part of the answer arising from "monopole bubbling" on ℝ³ can be understood as an equivariant integral over a Kronheimer-Nakajima moduli space of instantons on an orbifold of ℂ². It can also be described as a Witten index of a certain supersymmetric quiver quantum mechanics with N = (4; 4) supersymmetry. The map between the defect data and the quiver quantum mechanics is worked out for all values of N. For the SU(2) theory, we compute several examples of these line defect expectation values using the Witten index formula and confirm that the expressions agree with the formula derived by Okuda, Ito and Taki [16]. In addition, we present a Type IIB construction | involving D1-D3-NS5-branes | for monopole bubbling in N = 2* SU(N) SYM and demonstrate how the quiver quantum mechanics arises in this brane picture. [ABSTRACT FROM AUTHOR]