1. miR-185 inhibits non-small cell lung cancer cell proliferation and invasion through targeting of SOX9 and regulation of Wnt signaling
- Author
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Chao Sun, Wei Li, Hongcan Shi, Kai Liao, Lei Zhengwen, Xi Yu, Duonan Yu, Dan Lu, and Feiyang Shen
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Lung Neoplasms ,proliferation ,Kaplan-Meier Estimate ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,RNA interference ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,microRNA ,Genetics ,medicine ,Humans ,Neoplasm Invasiveness ,Autocrine signalling ,Molecular Biology ,3' Untranslated Regions ,Wnt Signaling Pathway ,non-small cell lung cancer ,Cell Proliferation ,Regulation of gene expression ,Binding Sites ,Oncogene ,microRNA-185 ,Base Sequence ,Wnt signaling pathway ,Cancer ,SOX9 Transcription Factor ,Articles ,Cell cycle ,Middle Aged ,medicine.disease ,Wnt signaling ,Cell biology ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,SRY-box 9 ,Female ,RNA Interference - Abstract
SRY-box 9 (SOX9) is an important transcription factor required for development, which has additionally been reported to be an independent prognostic indicator for the survival of patients with non‑small cell lung cancer (NSCLC). Accumulating evidence has indicated that dysregulation of microRNAs (miRNAs/miRs) may contribute to the initiation and progression of cancer. SOX9 may be regulated by a number of miRNAs in different types of cancer, including in NSCLC. The present study sought to identify novel candidate miRNAs associated with SOX9 in NSCLC using online tools, and investigated the detailed functions of miR‑185, which suppressed SOX9 mRNA expression most strongly out of the candidate miRNAs. It was observed that ectopic miR‑185 expression significantly suppressed NSCLC cell proliferation, invasion and migration. Using luciferase reporter gene and RNA immunoprecipitation assays, SOX9 was confirmed to be a direct target of miR‑185. In addition, the downstream Wnt signaling‑associated factors β‑catenin and c‑Myc proto‑oncogene protein (Myc) were demonstrated to be inhibited by miR‑185 overexpression. SOX9, β‑catenin and c‑Myc mRNA expression was significantly upregulated in NSCLC tissues, and was inversely correlated with miR‑185 expression. The results of the present study demonstrated that rescuing miR‑185 expression in NSCLC, thereby inhibiting SOX9 expression and the downstream Wnt signaling, and leading to the suppression of NSCLC cell proliferation, invasion and migration, may be a promising strategy for the treatment of NSCLC.
- Published
- 2017