1. Effect of non-adhering dressings on promotion of fibroblast proliferation and wound healing in vitro.
- Author
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Wiegand C, Abel M, Hipler UC, and Elsner P
- Subjects
- Animals, Carboxymethylcellulose Sodium pharmacology, Cell Line, Cell Survival drug effects, Colloids pharmacology, Cotton Fiber, Glycerol pharmacology, Humans, Petrolatum pharmacology, Polyesters pharmacology, Silicones pharmacology, Bandages standards, Cell Proliferation drug effects, Fibroblasts cytology, Wound Healing drug effects
- Abstract
Non-adhering dressings are commonly used during granulation, tissue formation, and re-epithelialization. Elucidating cytotoxic effects and influence on proliferation/migration capacity of cells like fibroblasts is of interest. Dressings' effects were investigated by comprehensive in vitro approach: (1) MTT assay measuring cell viability after direct contact, (2) ATP assay determining effects on cell proliferation, and (3) scratch wound assay featuring an in vitro wound healing model. One cotton-based dressing with vaseline (vas) was included in the study and four polyester dressings containing vas and technology-lipido-colloid matrix (TLC), carboxymethylcellulose (CMC), hydrocolloid (HC), or glycerin (gly) as additives. A polyamide dressing with vas + CMC and three silicone-based dressings (AT, CC, M) were tested. Polyester + vas + CMC did not negatively affect cell viability or proliferation but it was found that fibroblast layers appeared more irregular with decreased F-actin network structure and tubulin density possibly leading to hampered scratch closure. Silicone AT, polyester + gly and polyamide + vas + CMC caused distinct cell damage. The latter two further reduced cell viability, proliferation and scratch healing. From the overall results, it can be concluded that cotton + vas, polyester + TLC, polyester + vas + HC and the silicone dressings CC and M have the potential to prevent damage of newly formed tissue during dressing changes and positively influence wound healing.
- Published
- 2019
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