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This study was conducted to investigate the repair effect and mechanism of new platelet lysate film-forming spray (NPLFS) on rat skin burn wound model with a commonly used clinical dressing as the control. Forty-five SD rats were randomly divided into groups, including NPLFS treated (group A, n = 15), platelet-rich plasma (PRP) plaster group (group B, n = 15), and saline gauze cover group (group C, n = 15), fixed with 3M Tegaderm transparent film dressing. The healing rates on the 5th, 10th, 15th and 20th day, the histological hematoxylin and eosin (HE) staining changes on the 10th and 20th day, and the expression of CD31 on the 7th and 21th day after injury were observed. On the day 15 of treatment, the wounds in groups A and B were significantly reduced, while the degree of reduction in group C was not significant. On the day 20 of treatment, the wounds in groups A and B were completely healed and appeared flat, while the wounds in group C were only partly healed. On day 10, the HE staining showed significant increase in neovascularization in groups A and B as compared to group C (n = 3). The CD31 expression on day 20 after treatment showed that the integrated option densities (IODs) for rats in group A (20.13 ± 1.50) and group B (21.15 ± 0.79) were significantly higher than in group C (15.39 ± 1.41, P < 0.01). Therefore, NPLFS can promote the healing of burn wounds, and the effect is similar to that of PRP. [ABSTRACT FROM AUTHOR]

The ever-growing threats of bacterial infection and chronic wound healing have provoked an urgent need for novel antibacterial wound dressings. In this study, we developed a wound dressing for the treatment of infected wounds, which can reduce the inflammatory period (through the use of gentamycin sulfate (GS)) and enhance the granulation stage (through the addition of platelet-rich plasma (PRP)). Herein, the sustained antimicrobial CMC/GMs@GS/PRP wound dressings were developed by using gelatin microspheres (GMs) loading GS and PRP, covalent bonding to carboxymethyl chitosan (CMC). The prepared dressings exhibited high water uptake capability, appropriate porosity, excellent mechanical properties, sustain release of PRP and GS. Meanwhile, the wound dressing showed good biocompatibility and excellent antibacterial ability against Gram-negative and Gram-positive bacteria. Moreover, in vivo experiments further demonstrated that the prepared dressings could accelerate the healing process of E. coli and S. aureus -infected full-thickness wounds i n vivo, reepithelialization, collagen deposition and angiogenesis. In addition, the treatment of CMC/GMs@GS/PRP wound dressing could reduce bacterial count, inhibit pro-inflammatory factors (TNF-α, IL-1β and IL-6), and enhance anti-inflammatory factors (TGF-β1). The findings of this study suggested that biocompatible wound dressings with dual release of GS and PRP have great potential in the treatment of chronic and infected wounds. [ABSTRACT FROM AUTHOR]