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The most important phase in textile finishing processes is adsorption of chemical substances and compounds on textile materials surface and wettability as well. Interface phenomena happen between liquid and solid phase, like water solution and textile material, resulting in change of textile material surface free energy. Electrokinetic phenomena, as zeta potential and specific amount of surface charge, characterize electric charge of textile material, while contact angle characterize its wettability. The conventional processes of polyester modification, hydrolysis and aminolysis, improve properties of finished material but pollute the waste water as well. For that reason, nowadays the alternative procedures are investigated, such are treatments using ultrasound or different enzymes - lipase, catalase, etc. Therefore, in this paper the electrokinetic properties of polyester fabric after pre-treatment with enzymes - lipases ; commercial lipase Company BioCatalysts origin from Candida cylindracea, lipase, and laboratory obtained from Penicilium roqueforti is researched. The zeta potential, isoelectric point (IEP), a point of zero charge (PZC), the specific amount of surface charge, contact angle and water adsorption were determined on such treated polyester fabrics.