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6 results on '"Piorkowski G"'

1. Sequence Data From a Travel-Associated Case of Microcephaly Highlight a Persisting Risk due to Zika Virus Circulation in Thailand.

Author

Marquine S, Durand GA, Modenesi G, Khouadhria S, Piorkowski G, Badaut C, Canivez T, De Lamballerie X, Grard G, and Klitting R

Subjects
Humans, Pregnancy, Female, Travel, Thailand epidemiology, Phylogeny, Zika Virus genetics, Microcephaly, Zika Virus Infection, Pregnancy Complications, Infectious
Abstract

Zika virus has been circulating in Thailand since 2002 through continuous but likely low-level circulation. Here, we describe an infection in a pregnant woman who traveled to Thailand and South America during her pregnancy. By combining phylogenetic analysis with the patient's travel history and her pregnancy timeline, we confirmed that she likely got infected in Thailand at the end of 2021. This imported case of microcephaly highlights that Zika virus circulation in the country still constitutes a health risk, even in a year of lower incidence., Main Points: Here we trace the origin of travel-acquired microcephaly to Thailand, providing additional evidence that pre-American lineages of Zika virus can harm the fetus and highlighting that Zika virus constitutes a health threat even in a year of lower incidence., Competing Interests: Potential conflicts of interest . All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed., (© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)

Published
2024
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2. Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe.

Author

Durand GA, Piorkowski G, Thirion L, Ninove L, Giron S, Zandotti C, Denis J, Badaut C, Failloux AB, Grard G, Leparc-Goffart I, and de Lamballerie X

Subjects
Aedes virology, Animals, Asia, Disease Transmission, Infectious, Europe, Female, Humans, Male, Middle Aged, Mosquito Vectors virology, Polymorphism, Single Nucleotide, Zika Virus Infection virology, Genes, Viral genetics, Genotype, Zika Virus genetics, Zika Virus isolation & purification
Abstract

Three autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested PCR and sequencing in the Pr gene. Analysis of 16 genotype-specific Single Nucleotides Polymorphisms identified the Asian genotype and suggested a Southeast Asia origin.

Published
2020
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3. A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas.

Author

Moutailler S, Yousfi L, Mousson L, Devillers E, Vazeille M, Vega-Rúa A, Perrin Y, Jourdain F, Chandre F, Cannet A, Chantilly S, Restrepo J, Guidez A, Dusfour I, Vieira Santos de Abreu F, Pereira Dos Santos T, Jiolle D, Visser TM, Koenraadt CJM, Wongsokarijo M, Diallo M, Diallo D, Gaye A, Boyer S, Duong V, Piorkowski G, Paupy C, Lourenco de Oliveira R, de Lamballerie X, and Failloux AB

Subjects
Animals, Arbovirus Infections transmission, Arbovirus Infections virology, Arboviruses genetics, Arboviruses isolation & purification, Brazil, Cambodia, Disease Vectors, Epidemiological Monitoring, Female, French Guiana, Guadeloupe, Humans, Male, Molecular Epidemiology, Mosquito Vectors virology, Pilot Projects, RNA, Viral isolation & purification, Senegal, Suriname, Zika Virus genetics, Zika Virus Infection transmission, Culicidae virology, Endemic Diseases, Epidemics, High-Throughput Screening Assays methods, Zika Virus isolation & purification, Zika Virus Infection epidemiology
Abstract

Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.

Published
2019
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4. Evidence of Sexual Transmission of Zika Virus.

Author

D'Ortenzio E, Matheron S, Yazdanpanah Y, de Lamballerie X, Hubert B, Piorkowski G, Maquart M, Descamps D, Damond F, and Leparc-Goffart I

Subjects
Disease Transmission, Infectious, Humans, Middle Aged, Phylogeny, RNA, Viral analysis, RNA, Viral urine, Saliva virology, Semen virology, Urine virology, Young Adult, Zika Virus isolation & purification, Sexually Transmitted Diseases, Viral, Zika Virus genetics, Zika Virus Infection transmission
Published
2016
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5. Complete coding sequence of Zika virus from Martinique outbreak in 2015

Author

Piorkowski, G, Richard, P, Baronti, C, Gallian, P, Charrel, R, Leparc-Goffart, I, de Lamballerie, X, Emergence des Pathologies Virales (EPV), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Etablissement français du sang [Fort-de-France, Martinique] (EFS Martinique), Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA), Dubois Frid, Caroline, HAL AMU, Administrateur, Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université des Antilles et de la Guyane - UFR des sciences médicales (UAG UFR SM), Université des Antilles et de la Guyane (UAG), Assistance Publique - Hôpitaux de Marseille (APHM), Centre National de Référence (CNR) des Arbovirus - Laboratoire coordonnateur : Equipe Résidente de Recherche d'Infectiologie Tropicale (ERRIT), and Institut de Recherche Biomédicale des Armées Hôpital d’Instruction des Armées Laveran

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology, musculoskeletal diseases, outbreak, viruses, education, virus diseases, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, lcsh:Infectious and parasitic diseases, nervous system diseases, Zika virus, Aedes, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, lcsh:RC109-216, microcephaly, Americas, First Clinical Case Report
Abstract

International audience; Zika virus is an Aedes-borne Flavivirus causing fever, arthralgia, myalgia rash, associated with Guillain-Barré syndrome and suspected to induce microcephaly in the fetus. We report here the complete coding sequence of the first characterized Caribbean Zika virus strain, isolated from a patient from Martinique in December, 2015.

Published
2016
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6. Broad spectrum anti-flavivirus pyridobenzothiazolones leading to less infective virions

Author

Rolando Cannalire, Mario Milani, Giuseppe Manfroni, Géraldine Piorkowski, Serena Massari, Violetta Cecchetti, Maria Letizia Barreca, Tommaso Felicetti, Gilles Querat, Delia Tarantino, Tea Carletti, Alessandro Marcello, Stefano Sabatini, Oriana Tabarrini, Eloise Mastrangelo, Cannalire, Rolando, Tarantino, D., Piorkowski, G., Carletti, T., Massari, S., Felicetti, T., Barreca, M. L., Sabatini, S., Tabarrini, O., Marcello, A., Milani, M., Cecchetti, V., Mastrangelo, E., Manfroni, G., Querat, G., Università degli Studi di Perugia = University of Perugia (UNIPG), Sezione di Fisiologia e Biochimica delle Piante, Dipartimento di Biologia, Università degli Studi di Milano = University of Milan (UNIMI), Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Dipartimento di Biotecnologie e Bioscienze (Università di Milano-Bicocca), Université de Milan, Istituto di Biofisica del CNR, Emergence des Pathologies Virales (EPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Perugia (UNIPG), Università degli Studi di Milano [Milano] (UNIMI), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM), and HAL AMU, Administrateur

Subjects
0301 basic medicine, Pyridines, viruses, [SDV]Life Sciences [q-bio], Flavivirus replication, Virus Replication, Dengue fever, Zika virus, Antiviral small molecules, Flavivirus inhibitor, Flavivirus inhibitors, ComputingMilieux_MISCELLANEOUS, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Infectivity, biology, Yellow fever, Antivirals, 3. Good health, [SDV] Life Sciences [q-bio], Flavivirus, Antiviral small molecule, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Flavivirus inhibitors Antiviral small molecules Flavivirus replication Antivirals Dengue inhibitors Zika virus, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, RNA, Viral, Yellow fever virus, Dengue inhibitor, West Nile virus, Dengue inhibitors, 030106 microbiology, Antiviral Agents, Encephalitis Viruses, Tick-Borne, 03 medical and health sciences, Virology, medicine, Encephalitis Viruses, Animals, Humans, Antiviral, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Virus classification, Oxazocines, Pharmacology, Flaviviridae, Virion, Dengue Virus, Japanese encephalitis, medicine.disease, biology.organism_classification, 030104 developmental biology
Abstract

We report the design, synthesis, and biological evaluation of a class of 1H-pyrido[2,1-b][1,3]benzothiazol-1-ones originated from compound 1, previously identified as anti-flavivirus agent. Some of the new compounds showed activity in low ?M range with reasonable selectivity against Dengue 2, Yellow fever (Bolivia strain), and West Nile viruses. One of the most interesting molecules, compound 16, showed broad antiviral activity against additional flaviviruses such as Dengue 1, 3 and 4, Zika, Japanese encephalitis, several strains of Yellow fever, and tick-borne encephalitis viruses. Compound 16 did not exert any effect on alphaviruses and phleboviruses and its activity was maintained in YFV infected cells from different species. The activity of 16 appears specific for flavivirus with respect to other virus families, suggesting, but not proving, that it might be targeting a viral factor. We demonstrated that the antiviral effect of 16 is not related to reduced viral RNA synthesis or virion release. On the contrary, viral particles grown in the presence of 16 showed reduced infectivity, being unable to perform a second round of infection. The chemical class herein presented thus emerges as suitable to provide pan-flavivirus inhibitors.

Published
2019
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