1. Malakal virus from Africa and Kimberley virus from Australia are geographic variants of a widely distributed ephemerovirus.
- Author
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Blasdell KR, Voysey R, Bulach DM, Trinidad L, Tesh RB, Boyle DB, and Walker PJ
- Subjects
- Africa, Amino Acid Sequence, Animals, Australia, Cattle, Cell Line, Cricetinae, Ephemerovirus classification, Ephemerovirus isolation & purification, Gene Expression Profiling, Molecular Sequence Data, Multigene Family, Phylogeny, Phylogeography, Sequence Alignment, Sequence Homology, Amino Acid, Ephemerovirus genetics, Gene Expression, Genome, Viral
- Abstract
Kimberley virus (KIMV) is an arthropod-borne rhabdovirus that was isolated in 1973 and on several subsequent occasions from healthy cattle, mosquitoes (Culex annulirostris) and biting midges (Culicoides brevitarsis) in Australia. Malakal virus (MALV) is an antigenically related rhabdovirus isolated in 1963 from mosquitoes (Mansonia uniformis) in Sudan. We report here the complete genome sequences of KIMV (15442 nt) and MALV (15444 nt). The genomes have a similar organisation (3'-l-N-P-M-G-G(NS)-α1-α2-β-γ-L-t-5') to that of bovine ephemeral fever virus (BEFV). High levels of amino acid identity in each gene, similar gene expression profiles, clustering in phylogenetic analyses of the N, P, G and L proteins, and strong cross-neutralisation indicate that KIMV and MALV are geographic variants of the same ephemerovirus that, like BEFV, occurs in Africa, Asia and Australia., (Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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