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Vegetation optical depth (VOD), as a microwave-based vegetation index for vegetation water and biomass content, is increasingly used to study the impact of global climate and environmental changes on vegetation. Currently, VOD is mainly retrieved from passive microwave data and few studies focused on VOD retrievals from active microwave data. The Advanced SCATterometer (ASCAT) provides long-term C-band backscatter data at Vertical-Vertical (VV) polarization. In this study, a new ASCAT INRAE Bordeaux (IB) VOD (hereafter, IB VOD), was developed based on the Water Cloud Model (WCM) coupled with the Ulaby linear model for soil backscattering. The main features of IB VOD are that (i) the ERA5-Land soil moisture (SM) dataset was used as an auxiliary SM dataset in the retrievals, (ii) pixel-based soil model parameters were mapped using Random Forest (RF), and (iii) the vegetation model parameter was calibrated for each day. The IB VOD product was retrieved over Africa during 2015–2019, and its performances were evaluated in space and time by comparing with aboveground biomass (AGB), lidar tree height (TH), normalized difference vegetation index (NDVI), enhanced vegetation index (EVI) and leaf area index (LAI). Results were inter-compared with three other VOD products at the same frequency. In terms of spatial correlation with AGB (R = 0.92) and TH (R = 0.89), IB VOD outperforms the other VOD products, suggesting IB VOD has a strong ability to capture spatial patterns of AGB and TH. By comparing all VOD products against NDVI, EVI and LAI, we found that the highest temporal correlation with NDVI (EVI, LAI) was obtained with IB VOD over 29.94% (36.65%, 30.19%) of the study region. Considering all three vegetations indices, highest temporal correlation values with IB VOD could be particularly noted for deciduous broadleaf forests, woody savannas and savannas. • A new active VOD (IB VOD) retrieved from ASCAT is proposed. • Pixel-based soil model parameters were mapped using Random Forest (RF). • Daily variations in the vegetation model parameter are calibrated. • ASCAT IB VOD is compared to other C-band VOD products in Africa during 2015–2019. • ASCAT IB VOD performs well for predicting biomass and tree height. [ABSTRACT FROM AUTHOR]