1. Vitellaria paradoxa nutshells from seven sub-Saharan countries as potential herbal medicines for treating diabetes based on chemical compositions, HPLC fingerprints and bioactivity evaluation.
- Author
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Li D, Xiao JQ, Liu WY, Zhang CF, Akihisa T, Abe M, Masters ET, Zhai WW, Feng F, and Zhang J
- Subjects
- Africa South of the Sahara, Chromatography, High Pressure Liquid, Diabetes Mellitus enzymology, Glycoside Hydrolase Inhibitors chemistry, Glycoside Hydrolase Inhibitors pharmacology, Humans, Hypoglycemic Agents pharmacology, Molecular Docking Simulation, Plant Extracts pharmacology, Plants, Medicinal chemistry, alpha-Glucosidases metabolism, Diabetes Mellitus drug therapy, Hypoglycemic Agents chemistry, Plant Extracts chemistry, Sapotaceae chemistry
- Abstract
The aim of the study was to determine the feasibility of the Vitellaria paradoxa nutshell as a new medicinal resource for treating diabetes. A total of forty-one compounds were identified by HPLC-DAD-Q-TOF-MS and phytochemical methods in V. paradoxa nutshell methanol extract. Based on HPLC fingerprints, four characteristic constituents were quantified and the origin of twenty-eight V. paradoxa nutshells from seven sub-Saharan countries was compared, which were classified into three groups with chemometric method. Twenty-eight samples contained high total phenolic content, and exhibited moderate-higher antioxidant activity and strong α-glucosidase inhibitory activity. Furthermore, all fractions and isolated compounds were evaluated for their antioxidant and α-glucosidase inhibitory activities, and α-glucosidase inhibitory action mechanism of four characteristic constituents including protocatechuic acid, 3, 5, 7-trihydroxycoumarin, (2R, 3R)-(+)-taxifolin and quercetin was investigated via molecular docking method, which were all stabilized by hydrogen bonds with α-glucosidase. The study provided an effective approach to waste utilization of V. paradoxa nutshell, which would help to resolve waste environmental pollution and provide a basis for developing potential herbal resource for treating diabetes., (Copyright © 2019 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.)
- Published
- 2019
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