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The objective of this study is to determine the antimutagenic effect of honey and its correlation with the concentration of the total polyphenolic content. Seven honey samples were collected from different regions of Algeria. The total polyphenolic content was determined by Folin-Ciocalteu colorimetric assay. The antimutagenic effect was carried out by the AMES Salmonella/ microsome mutagenicity assay against three known mutagenic substances (4-nitro-o-phenylenediamine, sodium azide, and mitomycin C), using Salmonella typhimurium TA98, TA100, and TA102 strains. The results obtained revealed that Algerian honey contains high polyphenol content, which varied significantly between 38.04 and 286.28 µg of GAE/100 mg of honey. This variation is due to their different botanical and regional origins. In addition, all tested honey exhibited an antimutagenic effect against mutagenic substances; honey is effective to inhibit between 29.18±11.11 % and 73.14±11.14 % of mutagenic activity of chemical substances. There is a strong positive correlation between the total polyphenolic content and the antimutagenic proprieties of honey against the mutagenic substances. The results obtained suggest that honey is effective as an antimutagenic agent; it can play an important role in the protection of the mutagenic effect of DNA caused by chemical substances. [ABSTRACT FROM AUTHOR]

Processed meat products are presumptive sources of mutagens and genotoxins for consumers. Several epidemiological studies have reported that these products' high intakes have a positive link with cancer incidence. In Algeria, industrially processed meat products are widely consumed. However, there are no earlier studies involving their genotoxic activity. For this end, the current study aimed at evaluating the mutagenicity and the genotoxicity of some representative industrially processed meat products sold in popular supermarkets. All samples were extracted by established method, using both polar and non-polar solvents. The meat extracts mutagenicity was assessed by Ames test, using four strains of Salmonella typhimurium in the presence and absence of metabolic activation, and subsequently by treat and wash assay for extracts showing biologically significant results. The genotoxicity was determined in TK6 human lymphoblastoid cells using the in vitro micronucleus assay in micromethod. The results showed that all extracts analyzed induce no mutagenic activity. However, one of these extracts induced a biologically significant increase in the number of micronucleated cells. Our findings indicate the importance of the genetic damage detection for taking measures to suppress or reduce the exposure to harmful contaminants and encourage further research investigating genotoxic effects of industrially processed meat worldwide. • Industrially processed meat products are probable important source of mutagens. • Polar and non-polar extracts were prepared for mutagenic and genotoxic assessment. • No mutagenic activity was noted; a genotoxic potential was observed in one extract. • Genotoxic monitoring is of importance, concurrently to determination of contaminants. [ABSTRACT FROM AUTHOR]