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Due to its health-promoting properties, Artemisia campestris L. is an important crop widely used in the south Mediterranean basin as a food spice, and also in folk medicine. In this study, crude methanolic fraction was fractionated using two different solvents (ethyl-acetate AC and dichloromethane DCM). 19 individuals representing two different subspecies of A. campestris were investigated. A total of 86 compounds were identified. Several major and minor compounds were tentatively identified for the first time for this plant subspecies. The AC and DCM fractions exhibited different profiles compositions. Furthermore, both fractions presented a large variability of the percentage of their components. The AC fraction was rich in hydroxycinnamic acids and flavonoids aglycones. Major compounds for this fraction were 3,5-dicaffeoylquinic (7.0–50.03%), 4,5-dicaffeoylquinic acid derivative (0.11–11.15%) and luteolin (0–14.2%). Alternatively, for DCM fraction, it was mainly made up by flavonols and flavones classes'. The major identified components were 3- O -methylquercetin (8.7–19.1%), casticin III (0.0–9.3%), cirsiliol (3.89–14.0), eupatorin-5-methylether (3.4–11.5%), eupatorin (4.04–12.00%), eupafolin (1.4–9.55%) and 5,8,4′-trihydroxy-7-methoxyflavone (0.0–15.41%). Cluster analysis showed the presence two mains clusters and were depending on the solvent used. The chemical variation among the populations should lead to the selection of plants with high potential in order to use them in health care and food industry. • UHPLC-DAD-ESI-MSn analysis was used to assess the variability percentages of phenolic compounds. • Two different solvents were used for extraction fractionation: ethyl acetate and dichloromethane. • Several major and minor bioactive components were identified for the first time for this plant. • Major components were different and depended on solvent fractionation type. • Large variability of the percentages of main components was noticed for both fractions analyzed. [ABSTRACT FROM AUTHOR]

• Chemical and biological profile of Mentha rotundifolia essential oil. • The essential oil was analyzed by GC and GC–MS; a new chemotype was defined. • Considerable insecticide, repellent and antifungal activities were observed. • The essential oil has the potential to be developed as a novel botanical insecticide. • The vapor phase as a natural antifungal for the protection of cereal crops. The biological struggle by the use of the natural substances that have a pesticide effect may be an alternative to harmful chemicals; among these natural substances are the extracts of aromatic plants especially essential oils. The present work aims to highlight the fungicidal and insecticidal effect of the essential oil (EO), of an aromatic plant Mentha rotundifolia , against respectively, a phytopathogenic mold (Fusarium culmorum) and the adults of two pests (Sitophilus granarius and Tribolium confusum) of stored grains. The evaluation of the chemical composition of the EO was carried out by gas chromatography coupled to mass spectrometry (GC/MS). The analysis made it possible to identify 27 compounds, which corresponds to 97.93 % of the totality of the oil of which the majority compounds are: Piperitenone oxide 35.49 ± 0.08, Caryophyllene oxide 35.27 ± 0.13 and cis -Cinerolone 10.95 ± 0.05. The bioassays to assess the EO toxicity towards the pest insects revealed a very high effect, which increases significantly with the dose and the time of exposure. Similarly, the test for the repellent effect shows that this extract is a powerful insect repellent. In addition, we found that both the liquid and vapor phases of the EO present an inhibitory effect of the growth of the fungal strain. In the light of the results obtained, the plant studied is promising as a source of natural pesticides and lends itself well to research in the field of pest control using biochemical alternatives. [ABSTRACT FROM AUTHOR]