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The strong disturbance of upward mining of the short-distance multiseam results in frequent fractures in the coal seam to be mined, jeopardizing the operation safety due to the instability of the surrounding rock of the overlying stope and risks of coal and gas outburst. A lucrative alternative is the downward mining of multiseam, which wide implementation is limited by the lack of reliable data on stress evolution characteristics. In this paper, the evolution, nonlinear interaction, and superposition characteristics of mining-induced stress after multiple pressure relief in the short-distance multi-seam were investigated by the numerical simulation and physical simulation, taking the endowment and mining technology characteristics of group B short-distance multiseam in Pan'er Coal Mine in Anhui Province of China as an example. The numerical and physical models of multiple (2—5 times) mining activities in pressure relief multiseam (with five main coal layers) under downward mining are constructed and implemented with the FLAC3D software and 1 : 100 scaling, respectively. The results showed that a pressure relief arch, which bears and transfers stresses, is formed on the coal seam's roof and floor. In downward mining, the local arch expands to the long arch, which bearing capacity within the influence range of multiple mining disturbances is weak, and the arch structure is prone to instability. If a coal pillar is left in the upper coal seam after its mining, the lower coal seam mining will switch from the large-scale low-stress area under the goaf to the small-scale high-stress area of nonlinear interaction of the multiple mining stresses with the stress under the coal pillar. [ABSTRACT FROM AUTHOR]

Leber's hereditary optic neuropathy (LHON) is a maternally transmitted disease. Clinically, no efficient assay protocols have been available. In this study, we aimed to develop an oligonucleotide biochip specialized for detection of known base substitution mutations in mitochondrial DNA causing LHON and to investigate frequencies of LHON relevant variants in Anhui region of China. Thirty-two pairs of oligonucleotide probes matched with the mutations potentially linked to LHON were covalently immobilized. Cy5-lablled targets were amplified from blood DNA samples by a multiplex PCR method. Two kinds of primary mutations 11778 G > A and 14484 T > C from six confirmed LHON patients were interrogated to validate this biochip format. Further, fourteen Chinese LHON pedigrees and twenty-five unrelated healthy individuals were investigated by the LHON biochip, direct sequencing and pyrosequencing, respectively. The biochip was found to be able efficiently to discriminate homoplasmic and heteroplasmic mtDNA mutations in LHON. Biochip analysis revealed that twelve of eighteen LHON symptomatic cases from the 14 Chinese pedigree harbored the mutations either 11778G > A, 14484T > C or 3460G > A, respectively, accounting for 66.7%. The mutation 11778G > A in these patients was homoplasmic and prevalent (55.5%, 10 of 18 cases). The mutations 3460G > A and 3394T > C were found to co-exist in one LHON case. The mutation 13708G > A appeared in one LHON pedigree. Smaller amount of sampling and reaction volume, easier target preparation, fast and high-throughput were the main advantages of the biochip over direct DNA sequencing and pyrosequencing. Our findings suggested that primary mutations of 11778G > A, 14484T > C or 3460G > A are main variants of mtDNA gene leading to LHON in China. The biochip would easily be implemented in clinical diagnosis. [ABSTRACT FROM AUTHOR]