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Various ecological problems have become increasingly prominent due to the accelerated growth of urbanization. Ecological security and ecological conservation have become an important topics in the current scenario. This study took southern Anhui as an example, constructing comprehensive assessment models to conduct source identification from three perspectives, i.e. ecosystem services, ecological sensitivity and residents’ ecological needs. Landscape resistance surface was built based on the reciprocal of habitat quality and night-time light data. According to the circuit theory, the ecological process in the heterogeneous landscape was simulated to identify ecological corridors, extract pinch points and divide barriers that need improvement, thereby to construct the southern Anhui ecological security pattern (ESP). The pattern comprised 20 ecological sources, 37 ecological corridors, 9 pinch points and 2 levels of improvement areas. Specifically, ecological sources were mainly distributed within the area of Huangshan city and Xuancheng city, mostly covered with trees; ecological corridors were mostly located in the northern part of the research area; pinch points were mainly farmland or beside construction land; the primary improvement area was mainly in Chaohu city and Maanshan city, while the secondary improvement area was distributed around the primary area. The study discussed the diversified improvement strategies of different barriers and introduced the optimization scheme “one centre, two wings, one belt”, providing planning advice for decision-makers. The study expanded the construction of regional ESP, and partly guided the steady development of ESP of southern Anhui. [ABSTRACT FROM AUTHOR]