1. Dendritic cells modified with Der p1 antigen as a therapeutic potential for allergic rhinitis in a murine model via regulatory effects on IL-4, IL-10 and IL-13.
- Author
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Yu, Shaoqing, Jin, Ling, Che, Na, Zhang, Ruxin, Xu, Feifei, and Han, Bing
- Subjects
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IMMUNOGLOBULIN E , *DENDRITIC cells , *ALLERGIC rhinitis , *HOUSE dust mites , *NASAL mucosa , *T cells , *IMMUNOLOGICAL tolerance - Abstract
Abstract Objectives House dust mites, including Der p1, are common allergens. The current study was designed to explore the allergen-specific immune tolerance effects of Der p1-modified dendritic cells (DCs) through IL-4, IL-10 and IL-13 on an allergic rhinitis (AR) mouse model. Methods A lentivirus was modified to express Derp1. Then, immature DCs from mice were infected with this modified lentivirus to generate a lenti- Derp1 -GFP DCs. 24 mice were random divided into four groups (n = 6 each), AR mouse were sensitized by Derp1 allergens and treated with lenti-GFP DCs (GFP-DC/AR group), or lenti- Derp1 -GFP DCs (Der p1-DC/AR group) and dexamethasone (Dex/AR group), mice in the control group were treated with PBS instead of Der p1 then also intraperitoneally injected with 5 × 106 lenti-GFP DCs/mouse. AR symptoms expressed by each mouse were recorded. The proportions of CD4+CD25+Foxp3+ regulatory T cells among CD4+ T cells in the peripheral blood, and mRNA and protein expression levels of IL-4, IL-10, and IL-13 were measured. Results DCs infected with lenti- Derp1 -GFP stimulated the maturation of DCs. Compared with the GFP-DC/AR group, mice in the Der p1-DC/AR group showed an ameliorated allergic response, a significant decrease in the levels of serum IgE, IgG1, and histamine, and a decrease in the expression of IL-4 and IL-13 mRNA and protein in the nasal mucosa. The expression of IL-10 increased in the Der p1-DC/AR group to a level similar to that observed in the Dex/AR group. Conclusions These results indicate that Der p1-modified DCs have therapeutic potential for AR via downregulation of IL-4 and IL-13, and upregulation of IL-10. Highlights • Immature DCs were infected with Der p1 gene change into Derp1 -DC. • Der p1 -DC ameliorated allergic response of AR via regulatory effects on IL-4, IL-10 and IL-13. • Der p1 -DC increased the percentage of CD4+CD25+Foxp3+ Tregs. • Der p1 -DC has the same therapeutic effect of glucocorticoid treatment in AR. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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