1. Genomics- and Transcriptomics-Guided Discovery of Clavatols from Arctic Fungi Penicillium sp. MYA5.
- Author
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Sun YY, Hu B, Yu HB, Zhou J, Meng XC, Ning Z, Ding JF, Cui MH, and Liu XY
- Subjects
- Genomics methods, Escherichia coli drug effects, Escherichia coli genetics, Microbial Sensitivity Tests, Transcriptome, Arctic Regions, Siderophores pharmacology, Aldehyde Reductase antagonists & inhibitors, Aldehyde Reductase genetics, Penicillium genetics, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Staphylococcus aureus drug effects
- Abstract
Clavatols exhibit a wide range of biological activities due to their diverse structures. A genome mining strategy identified an A5cla cluster from Penicillium sp. MYA5, derived from the Arctic plant Dryas octopetala , is responsible for clavatol biosynthesis. Seven clavatols, including one new clavatol derivate named penicophenone F ( 1 ) and six known clavatols ( 2 - 7 ), were isolated from Penicillium sp. MYA5 using a transcriptome mining strategy. These structures were elucidated by comprehensive spectroscopic analysis. Antibacterial, aldose reductase inhibition, and siderophore-producing ability assays were conducted on compounds 1 - 7 . Compounds 1 and 2 demonstrated inhibitory effects on the ALR2 enzyme with inhibition rates of 75.3% and 71.6% at a concentration of 10 μM, respectively. Compound 6 exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 4.0 μg/mL and 4.0 μg/mL, respectively. Additionally, compounds 1 , 5 , and 6 also showed potential iron-binding ability.
- Published
- 2024
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