1. Molecular diversity of Moraxella bovis isolated from Brazil, Argentina and Uruguay over a period of three decades.
- Author
-
Conceição FR, Dellagostin OA, Paolichi F, Leturia AC, and Gil Turnes C
- Subjects
- Animals, Argentina epidemiology, Brazil epidemiology, Cattle, Cattle Diseases epidemiology, Cross Reactions, DNA, Bacterial analysis, Disease Outbreaks veterinary, Keratoconjunctivitis epidemiology, Keratoconjunctivitis microbiology, Moraxella bovis classification, Moraxella bovis isolation & purification, Moraxellaceae Infections epidemiology, Moraxellaceae Infections microbiology, Random Amplified Polymorphic DNA Technique veterinary, Uruguay epidemiology, Cattle Diseases microbiology, Genetic Variation, Keratoconjunctivitis veterinary, Moraxella bovis genetics, Moraxellaceae Infections veterinary
- Abstract
The molecular profile of 30 Moraxella bovis strains, recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil and Uruguay between 1974 and 2001, was determined through randomly applied polymorphic DNA (RAPD) analysis. Molecular profiles of nine strains recovered after 1990 varied from those recovered before 1990. The profiles of 13 strains (48%) differed from those of three vaccinal strains extensively used since 1984 in Argentina and Uruguay. Eight Argentinean strains, one from Brazil and two from Uruguay had identical RAPD profiles. Strains belonging to different serogroups had identical RAPD profiles, demonstrating that this technique was not able to discriminate among strains with low cross-reactivity indices. RAPD may be helpful in the primary characterization of M. bovis strains, but it does not replace serological characterization.
- Published
- 2004
- Full Text
- View/download PDF