1. Argentine strain of equine herpesvirus 1 isolated from an aborted foetus shows low virulence in mouse respiratory and abortion models.
- Author
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Galosi CM, Barbeito CG, Vila Roza MV, Cid de la Paz V, Ayala MA, Corva SG, Etcheverrigaray ME, and Gimeno EJ
- Subjects
- Animals, Antibodies, Viral blood, Argentina, Body Weight, DNA, Viral chemistry, DNA, Viral genetics, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay veterinary, Female, Fetal Death pathology, Fetal Death virology, Herpesviridae Infections pathology, Herpesviridae Infections virology, Herpesvirus 1, Equid genetics, Herpesvirus 1, Equid growth & development, Herpesvirus 1, Equid isolation & purification, Horse Diseases pathology, Horses, Immunohistochemistry veterinary, Lung pathology, Lung virology, Mice, Mice, Inbred BALB C, Polymerase Chain Reaction veterinary, Pregnancy, Specific Pathogen-Free Organisms, Virulence, Virus Replication, Fetal Death veterinary, Herpesviridae Infections veterinary, Herpesvirus 1, Equid pathogenicity, Horse Diseases virology
- Abstract
The equine herpesvirus 1 (EHV-1) was isolated in Argentina from an aborted equine foetus in 1979. This virus (SPv) has special restriction patterns (RP) in comparison with other Argentine isolates. In addition, SPv could be distinguished on the basis of its pathogenicity in baby mice inoculated intracerebrally. We studied the growth properties of the SPv in cell culture and its effects in a mouse respiratory and abortion model. We observed that SPv did not modify its capacity to grow in cell culture with respect to reference HH1 strain. Nevertheless, we found significant differences between the titres of the two strains at 8-14 h post-infection (PI). In this work we demonstrated that SPv showed low virulence in female at different stages of gestation, consistently, with results found in the mouse respiratory model. We considered that this low virulence of SPv could be related to its RP because the RP of HH1 strain are similar to those of the HVS25A strain and both showed effect on pregnant mice. More specific studies about genomic alterations to the SPv are necessary for identifying, more clearly, if the intra-strain variations have relation with the low virulence in the mouse respiratory and abortion model.
- Published
- 2004
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